Prevalence of verotoxigenic Escherichia coli O157 (VTEC O157) and compliance with microbiological safety standards in bovine carcasses from an industrial beef slaughter plant


Por: Ramoneda, M, Foncuberta, M, Simon, M, Sabate, S, Ferrer, MD, Herrera, S, Landa, B, Muste, N, Marti, R, Trabado, V, Carbonell, O, Vila, M, Espelt, M, Ramirez, B, Duran, J

Publicada: 1 jun 2013
Resumen:
The presence of Salmonella spp. and levels of Enterobacteriaceae and aerobic plate count were determined in 300 bovine carcasses randomly collected in an industrial cattle slaughterhouse in Catalonia (Spain) as part of a control programme to validate good slaughter practices according to Commission Regulation No 2073/2005. The verotoxigenic Escherichia coli O157 (VTEC O157), although not currently legislated, was also investigated in the same carcasses due to the importance of bovines as a reservoir for this micro-organism. Virulence genes (vtx1, vtx2 and eae), the presence of fliCH7 and antimicrobial susceptibility were studied in E.coli O157 isolates. Levels of Enterobacteriaceae and aerobic colonies and the presence of Salmonella were within the admissible range stipulated by current legislation. However, VTEC O157 was detected in 14 center dot 7% of carcasses. Among the VTEC O157 strains tested for antimicrobial susceptibility, 65% were multiresistant. Overall, the results of this study indicate that even with good manufacturing practices, contamination with VTEC O157 can occur and cattle meat can pose a risk to human health. These results confirm the need for a review of the appropriateness of introducing antimicrobial treatments in the processing of cattle carcasses in Europe.

Filiaciones:
Ramoneda, M:
 ASPB, Barcelona 08023, Spain

Foncuberta, M:
 ASPB, Barcelona 08023, Spain

 CIBER Epidemiol & Publ Hlth CIBERESP, Madrid, Spain

Simon, M:
 ASPB, Barcelona 08023, Spain

Sabate, S:
 ASPB, Barcelona 08023, Spain

Ferrer, MD:
 ASPB, Barcelona 08023, Spain

Herrera, S:
 Inst Salud Carlos III, Natl Ctr Microbiol, Madrid, Spain

Landa, B:
 ASPB, Barcelona 08023, Spain

Muste, N:
 ASPB, Barcelona 08023, Spain

Marti, R:
 ASPB, Barcelona 08023, Spain

Trabado, V:
 ASPB, Barcelona 08023, Spain

Carbonell, O:
 ASPB, Barcelona 08023, Spain

Vila, M:
 ASPB, Barcelona 08023, Spain

Espelt, M:
 ASPB, Barcelona 08023, Spain

Ramirez, B:
 ASPB, Barcelona 08023, Spain

Duran, J:
 ASPB, Barcelona 08023, Spain
ISSN: 02668254





LETTERS IN APPLIED MICROBIOLOGY
Editorial
WILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA, Reino Unido
Tipo de documento: Article
Volumen: 56 Número: 6
Páginas: 408-413
WOS Id: 000319071800003
ID de PubMed: 23461411
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