A New Variant of the aadE-sat4-aphA-3 Gene Cluster Found in a Conjugative Plasmid from a MDR Campylobacter jejuni Isolate
Por:
Guirado, P, Miro, E, Iglesias-Torrens, Y, Navarro, F, Campoy, S, Alioto, TS, Gomez-Garrido, J, Madrid, C, Balsalobre, C
Publicada:
1 abr 2022
Resumen:
Campylobacter jejuni is a foodborne pathogen causing bacterial gastroenteritis, with the highest incidence reported in Europe. The prevalence of antibiotic resistance in C. jejuni, as well as in many other bacterial pathogens, has increased over the last few years. In this report, we describe the presence of a plasmid in a multi-drug-resistant C. jejuni strain isolated from a gastroenteritis patient. Mating experiments demonstrated the transference of this genetic element (pCjH01) among C. jejuni by plasmid conjugation. The pCjH01 plasmid was sequenced and assembled, revealing high similarity (97% identity) with pTet, a described tetracycline resistance encoding plasmid. pCjH01 (47.7 kb) is a mosaic plasmid composed of a pTet backbone that has acquired two discrete DNA regions. Remarkably, one of the acquired sequences carried an undescribed variant of the aadE-sat4-aphA-3 gene cluster, providing resistance to at least kanamycin and gentamycin. Aside from the antibiotic resistance genes, the cluster also carries genes coding for putative regulators, such as a sigma factor of the RNA polymerase and an antisigma factor. Homology searches suggest that Campylobacter exchanges genetic material with distant G-positive bacterial genera.
Filiaciones:
Guirado, P:
Univ Barcelona, Sch Biol, Dept Genet Microbiol & Stat, Av Diagonal 643, Barcelona 08028, Spain
Miro, E:
Hosp Santa Creu & Sant Pau, Inst Invest Biomed St Pau IIB St Pau, St Quinti 89, Barcelona 08041, Spain
Iglesias-Torrens, Y:
Hosp Santa Creu & Sant Pau, Inst Invest Biomed St Pau IIB St Pau, St Quinti 89, Barcelona 08041, Spain
Univ Autonoma Barcelona, Dept Genet & Microbiol, Cerdanyola Del Valles 08193, Spain
Navarro, F:
Hosp Santa Creu & Sant Pau, Inst Invest Biomed St Pau IIB St Pau, St Quinti 89, Barcelona 08041, Spain
Campoy, S:
Univ Autonoma Barcelona, Dept Genet & Microbiol, Cerdanyola Del Valles 08193, Spain
Alioto, TS:
Barcelona Inst Sci & Technol BIST, Ctr Genom Regulat CRG, CNAG CRG, Baldiri & Reixac 4, Barcelona 08028, Spain
Univ Pompeu Fabra UPF, Barcelona 08002, Spain
Gomez-Garrido, J:
Barcelona Inst Sci & Technol BIST, Ctr Genom Regulat CRG, CNAG CRG, Baldiri & Reixac 4, Barcelona 08028, Spain
Madrid, C:
Univ Barcelona, Sch Biol, Dept Genet Microbiol & Stat, Av Diagonal 643, Barcelona 08028, Spain
Balsalobre, C:
Univ Barcelona, Sch Biol, Dept Genet Microbiol & Stat, Av Diagonal 643, Barcelona 08028, Spain
gold, Green Published, All Open Access, Gold, Green
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