Functional consequences of platelet binding to T lymphocytes in inflammation
Por:
Zamora, C, Canto, E, Nieto, JC, Ortiz, MA, Diaz-Torne, C, Diaz-Lopez, C, Llobet, JM, Juarez, C, Vidal, S
Publicada:
1 sep 2013
Resumen:
Platelet binding is a new regulatory mechanism of T cell function; higher frequency of platelet binding to lymphocytes is associated with low severe inflammatory profile. Expression of the scavenger receptor CD36 on lymphocytes is intriguing. We observed that a minor subpopulation of lymphocytes expressed CD36 on the cell surface. We investigated the source of CD36 and also the proliferation and cytokine production of these CD36(+) CD4(+) lymphocytes. Flow cytometry analysis and immunofluorescence microscopy showed that CD36(+) platelets were responsible for CD36 detection on lymphocytes. CD36 was then used as a tool to characterize lymphocytes with bound platelets. Activation-induced proliferation was lower in CD4(+) lymphocytes with bound platelets than lymphocytes without bound platelets. IL-17 and IFN- production was also reduced in lymphocytes with bound platelets. We then studied the presence of CD36(+) CD4(+) lymphocytes in RA patients. We observed that the percentage of CD4(+) lymphocytes with bound platelets was higher on RA patients than in healthy donors. RA patients with higher titers of anti-CCP, RF levels, and cardiovascular risk index presented a lower percentage of CD4(+) lymphocytes with bound platelets. These patients also had higher IL-17 and IFN- production. These results suggest that platelet-binding modifies lymphocyte function. This binding could be a regulatory mechanism in RA that confers a less severe phenotype.
Filiaciones:
Zamora, C:
IIB St Pau, Inst Recerca, Dept Immunol, Barcelona, Spain
Canto, E:
IIB St Pau, Inst Recerca, Dept Immunol, Barcelona, Spain
Nieto, JC:
IIB St Pau, Inst Recerca, Dept Immunol, Barcelona, Spain
Ortiz, MA:
IIB St Pau, Inst Recerca, Dept Immunol, Barcelona, Spain
Diaz-Torne, C:
Hosp Santa Creu & Sant Pau, Rheumatol Unit, Barcelona 08025, Spain
Diaz-Lopez, C:
Hosp Santa Creu & Sant Pau, Rheumatol Unit, Barcelona 08025, Spain
Llobet, JM:
Hosp Santa Creu & Sant Pau, Rheumatol Unit, Barcelona 08025, Spain
Juarez, C:
Hosp Santa Creu & Sant Pau, Dept Immunol, Barcelona 08025, Spain
Vidal, S:
IIB St Pau, Inst Recerca, Dept Immunol, Barcelona, Spain
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