Low Cerebrospinal Fluid Concentration of Mitochondrial DNA in Preclinical Alzheimer Disease
Por:
Podlesniy, P, Figueiro-Silva, J, Llado, A, Antonell, A, Sanchez-Valle, R, Alcolea, D, Lleo, A, Molinuevo, JL, Serra, N, Trullas, R
Publicada:
1 nov 2013
Resumen:
ObjectiveTo identify a novel biochemical marker that precedes clinical symptoms in Alzheimer disease (AD).
MethodsUsing quantitative polymerase chain reaction techniques, we measured circulating cell-free mitochondrial DNA (mtDNA) in cerebrospinal fluid (CSF) from study participants, selected from a cohort of 282 subjects, who were classified according to their concentrations of amyloid (1-42), total tau, and phosphorylated tau and by the presence or absence of dementia, into asymptomatic subjects at risk of AD, symptomatic patients diagnosed with sporadic AD, presymptomatic subjects carrying pathogenic PSEN1 mutations, and patients diagnosed with frontotemporal lobar degeneration (FTLD). We performed equivalent studies in a separate validation cohort of sporadic AD and FTLD patients. In addition, we measured mtDNA copy number in cultured cortical neurons from mutant amyloid precursor protein/presenilin1 (APP/PS1) transgenic mice.
ResultsAsymptomatic patients at risk of AD and symptomatic AD patients, but not FTLD patients, exhibit a significant decrease in circulating cell-free mtDNA in the CSF. These observations were confirmed in the validation cohort. In addition, presymptomatic subjects carrying pathogenic PSEN1 gene mutations show low mtDNA content in CSF before the appearance of AD-related biomarkers in CSF. Moreover, mtDNA content in CSF discriminates with high sensitivity and specificity AD patients from either controls or patients with FTLD. Furthermore, cultured cortical neurons from APP/PS1 transgenic mice have fewer mtDNA copies before the appearance of altered synaptic markers.
InterpretationLow content of mtDNA in CSF may be a novel biomarker for the early detection of preclinical AD. These findings support the hypothesis that mtDNA depletion is a characteristic pathophysiological factor of neurodegeneration in AD. Ann Neurol 2013;74:655-668
Filiaciones:
Podlesniy, P:
CSIC, Inst Invest Biomed Barcelona, Neurobiol Unit, Barcelona, Spain
CIBERNED, Ctr Invest Biomed Red Enfermedades Neurodegenerat, Barcelona, Spain
Figueiro-Silva, J:
CSIC, Inst Invest Biomed Barcelona, Neurobiol Unit, Barcelona, Spain
CIBERNED, Ctr Invest Biomed Red Enfermedades Neurodegenerat, Barcelona, Spain
Llado, A:
Hosp Clin Barcelona, Neurol Serv, Barcelona, Spain
IDIBAPS, Inst Invest Biomed August Pi & Sunyer, Barcelona, Spain
Antonell, A:
Hosp Clin Barcelona, Neurol Serv, Barcelona, Spain
IDIBAPS, Inst Invest Biomed August Pi & Sunyer, Barcelona, Spain
Sanchez-Valle, R:
Hosp Clin Barcelona, Neurol Serv, Barcelona, Spain
IDIBAPS, Inst Invest Biomed August Pi & Sunyer, Barcelona, Spain
Alcolea, D:
CIBERNED, Ctr Invest Biomed Red Enfermedades Neurodegenerat, Barcelona, Spain
Hosp Santa Creu & Sant Pau, Dept Neurol, Barcelona, Spain
Lleo, A:
CIBERNED, Ctr Invest Biomed Red Enfermedades Neurodegenerat, Barcelona, Spain
Hosp Santa Creu & Sant Pau, Dept Neurol, Barcelona, Spain
Molinuevo, JL:
Hosp Clin Barcelona, Neurol Serv, Barcelona, Spain
IDIBAPS, Inst Invest Biomed August Pi & Sunyer, Barcelona, Spain
Serra, N:
CSIC, Inst Invest Biomed Barcelona, Neurobiol Unit, Barcelona, Spain
CIBERNED, Ctr Invest Biomed Red Enfermedades Neurodegenerat, Barcelona, Spain
Trullas, R:
CSIC, Inst Invest Biomed Barcelona, Neurobiol Unit, Barcelona, Spain
CIBERNED, Ctr Invest Biomed Red Enfermedades Neurodegenerat, Barcelona, Spain
IDIBAPS, Inst Invest Biomed August Pi & Sunyer, Barcelona, Spain
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