Non-meiotic chromosome instability in human immature oocytes
Por:
Daina, G, Ramos, L, Rius, M, Obradors, A, del Rey, J, Giralt, M, Campillo, M, Velilla, E, Pujol, A, Martinez-Pasarell, O, Benet, F, Navarro, J
Publicada:
1 feb 2014
Resumen:
Aneuploidy has been a major issue in human gametes and is closely related to fertility problems, as it is known to be present in cleavage stage embryos and gestational losses. Pre-meiotic chromosome abnormalities in women have been previously described. The aim of this study is to assess the whole-chromosome complement in immature oocytes to find those abnormalities caused by mitotic instability. For this purpose, a total of 157 oocytes at the germinal vesicle or metaphase I stage, and discarded from IVF cycles, were analysed by CGH. Fifty-six women, between 18 and 45 years old (mean 32.5 years), including 32 IVF patients (25-45 years of age) and 24 IVF oocyte donors (18-33 years of age), were included in the study. A total of 25/157 (15.9%) of the oocytes analysed, obtained from three IVF clinics, contained chromosome abnormalities, including both aneuploidy (24/157) and structural aberrations (9/157). Independently of the maternal age, the incidence of abnormal oocytes which originated before meiosis is 15.9%, and these imbalances were found in 33.9% of the females studied. This work sheds light on the relevance of mitotic instability responsible for the generation of the abnormalities present in human oocytes.
Filiaciones:
Daina, G:
Univ Autonoma Barcelona, Catedra Recerca Eugin UAB, Bellaterra 08193, Spain
Univ Autonoma Barcelona, Fac Med, Dept Biol Cellular Fisiol & Immunol, Unitat Biol Cellular & Genet Med, Bellaterra 08193, Spain
Ramos, L:
Univ Autonoma Barcelona, Fac Med, Dept Biol Cellular Fisiol & Immunol, Unitat Biol Cellular & Genet Med, Bellaterra 08193, Spain
Rius, M:
Univ Autonoma Barcelona, Fac Med, Dept Biol Cellular Fisiol & Immunol, Unitat Biol Cellular & Genet Med, Bellaterra 08193, Spain
Obradors, A:
Univ Autonoma Barcelona, Catedra Recerca Eugin UAB, Bellaterra 08193, Spain
Clin Eugin, Barcelona, Spain
del Rey, J:
Univ Autonoma Barcelona, Catedra Recerca Eugin UAB, Bellaterra 08193, Spain
Univ Autonoma Barcelona, Fac Med, Dept Biol Cellular Fisiol & Immunol, Unitat Biol Cellular & Genet Med, Bellaterra 08193, Spain
Giralt, M:
Univ Autonoma Barcelona, Fac Med, Dept Biol Cellular Fisiol & Immunol, Unitat Biol Cellular & Genet Med, Bellaterra 08193, Spain
Campillo, M:
Univ Autonoma Barcelona, Lab Med Computac, Unitat Bioestadist, E-08193 Barcelona, Spain
Velilla, E:
Inst Marques Servei Biol Reprod, Barcelona, Spain
Pujol, A:
Clin Eugin, Barcelona, Spain
Martinez-Pasarell, O:
Fundacio Puigvert Hosp St Pau & Santa Creu, Barcelona, Spain
Benet, F:
Univ Autonoma Barcelona, Catedra Recerca Eugin UAB, Bellaterra 08193, Spain
Univ Autonoma Barcelona, Fac Med, Dept Biol Cellular Fisiol & Immunol, Unitat Biol Cellular & Genet Med, Bellaterra 08193, Spain
Navarro, J:
Univ Autonoma Barcelona, Catedra Recerca Eugin UAB, Bellaterra 08193, Spain
Univ Autonoma Barcelona, Fac Med, Dept Biol Cellular Fisiol & Immunol, Unitat Biol Cellular & Genet Med, Bellaterra 08193, Spain
Green Published, Bronze
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