Methods for the identification and characterization of extracellular vesicles in cardiovascular studies: from exosomes to microvesicles
Por:
Davidson S.M., Boulanger C.M., Aikawa E., Badimon L., Barile L., Binder C.J., Brisson A., Buzas E., Emanueli C., Jansen F., Katsur M., Lacroix R., Lim S.K., Mackman N., Mayr M., Menasché P., Nieuwland R., Sahoo S., Takov K., Thum T., Vader P., Wauben, MHM, Witwer K., Sluijter, JPG
Publicada:
1 ene 2023
Ahead of Print:
1 mar 2022
Resumen:
Extracellular vesicles (EVs) are nanosized vesicles with a lipid bilayer that are released from cells of the cardiovascular system, and are considered important mediators of intercellular and extracellular communications. Two types of EVs of particular interest are exosomes and microvesicles, which have been identified in all tissue and body fluids and carry a variety of molecules including RNAs, proteins, and lipids. EVs have potential for use in the diagnosis and prognosis of cardiovascular diseases and as new therapeutic agents, particularly in the setting of myocardial infarction and heart failure. Despite their promise, technical challenges related to their small size make it challenging to accurately identify and characterize them, and to study EV-mediated processes. Here, we aim to provide the reader with an overview of the techniques and technologies available for the separation and characterization of EVs from different sources. Methods for determining the protein, RNA, and lipid content of EVs are discussed. The aim of this document is to provide guidance on critical methodological issues and highlight key points for consideration for the investigation of EVs in cardiovascular studies.
Filiaciones:
Davidson S.M.:
UCL, Hatter Cardiovasc Inst, London WC1E 6HX, England
Boulanger C.M.:
Univ Paris Cite, INSERM, Paris Cardiovasc Res Ctr, Paris, France
Aikawa E.:
Harvard Med Sch, Dept Med, Brigham & Womens Hosp, Ctr Excellence Vasc Biol, Boston, MA 02115 USA
Badimon L.:
Autonomous Univ Barcelona, IR Hosp Santa Creu & Santa Pau IIBSantPau, CiberCV, Cardiovasc Sci Program ICCC, Barcelona, Spain
Barile L.:
Univ Svizzera Italiana, Fac Biomed Sci, CH-6900 Lugano, Switzerland
Univ Svizzera Italiana, Ente Osped Cantonale, Ist Cardioctr Ticino, Lab Cardiovasc Theranost, CH-6900 Lugano, Switzerland
Binder C.J.:
Med Univ Vienna, Dept Lab Med, Vienna, Austria
Brisson A.:
Univ Bordeaux IPB, CNRS, Mol Imaging & NanoBioTechnol, UMR 5248 CBMN, Bat B14,Allee Geoffroy St Hilaire, F-33600 Pessac, France
Buzas E.:
Semmelweis Univ, Dept Genet Cell & Immunobiol, HCEMM SU & ELKH SE Immune Proteogen Extracellular, Budapest, Hungary
Emanueli C.:
Imperial Coll London, Natl Heart & Lung Inst, Hammersmith Campus, London W12 0NN, England
Jansen F.:
Univ Hosp Bonn, Heart Ctr, Dept Internal Med 2, Bonn, Germany
Katsur M.:
UCL, Hatter Cardiovasc Inst, London WC1E 6HX, England
Lacroix R.:
CHU La Concept, AP HM, Dept Haematol & Vasc Biol, Marseille, France
Aix Marseille Univ, Ctr Rech CardioVasc & Nutr C2VN, Inst Natl Rech Agr Alimentat & Environm INRAE, INSERM 1263, Marseille, France
Lim S.K.:
Agcy Sci Technol & Res, Inst Mol & Cell Biol, Singapore, Singapore
Agcy Sci Technol & Res, Inst Med Biol, Singapore, Singapore
Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Surg, Singapore, Singapore
Mackman N.:
Univ N Carolina, Dept Med, UNC Blood Res Ctr, Chapel Hill, NC USA
Mayr M.:
Kings Coll London, Sch Cardiovasc Med & Sci, British Heart Fdn Ctr, London, England
Menasché P.:
Hop Europeen Georges Pompidou, Dept Cardiovasc Surg, Paris, France
Univ Utrecht, Univ Med Ctr Utrecht, UMC Utrecht Regenerat Med Ctr, Lab Expt Cardiol,Dept Cardiol, Utrecht, Netherlands
Univ Utrecht, Univ Med Ctr Utrecht, Circulatory Hlth Lab, Utrecht, Netherlands
Nieuwland R.:
Univ Amsterdam, Lab Expt Clin Chem, Amsterdam UMC, Amsterdam, Netherlands
Univ Amsterdam, Vesicle Observat Ctr, Amsterdam UMC, Amsterdam, Netherlands
Sahoo S.:
Icahn Sch Med Mt Sinai, Cardiovasc Res Inst, New York, NY USA
Takov K.:
Kings Coll London, Sch Cardiovasc Med & Sci, British Heart Fdn Ctr, London, England
Thum T.:
Fraunhofer Inst Toxicol & Expt Med, Hannover, Germany
Hannover Med Sch, Inst Mol & Translat Therapeut Strategies, Hannover, Germany
Vader P.:
Univ Paris Cite, INSERM, Paris Cardiovasc Res Ctr, Paris, France
Univ Med Ctr Utrecht, CDL Res, Heidelberglaan 100, NL-3584 CX Utrecht, Netherlands
Wauben, MHM:
Univ Utrecht, Fac Vet Med, Dept Biomol Hlth Sci, Yalelaan 2, Utrecht, Netherlands
Witwer K.:
Johns Hopkins Univ, Sch Med, Dept Mol & Comparat Pathobiol, Baltimore, MD USA
Johns Hopkins Univ, Sch Med, Dept Neurol, Baltimore, MD 21205 USA
Sluijter, JPG:
Univ Utrecht, Univ Med Ctr Utrecht, UMC Utrecht Regenerat Med Ctr, Lab Expt Cardiol,Dept Cardiol, Utrecht, Netherlands
Univ Utrecht, Univ Med Ctr Utrecht, Circulatory Hlth Lab, Utrecht, Netherlands
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