Importance of cerebrospinal fluid storage conditions for the Alzheimer's disease diagnostics on an automated platform
Por:
Ferrer, R, Zhu, N, Arranz, J, Porcel, I, El Bounasri, S, Sanchez, O, Torres, S, Julve, J, Lleo, A, Blanco-Vaca, F, Alcolea, D, Tondo, M
Publicada:
27 jun 2022
Ahead of Print:
1 abr 2022
Resumen:
Objectives Alzheimer's disease (AD) is considered the most common cause of dementia in older people. Cerebrospinal fluid (CSF) A beta 1-42, A beta 1-40, total Tau (t-Tau), and phospho Tau (p-Tau) are important biomarkers for the diagnosis, however, they are highly dependent on the pre-analytical conditions. Our aim was to investigate the potential influence of different storage conditions on the simultaneous quantification of these biomarkers in a fully-automated platform to accommodate easier pre-analytical conditions for laboratories. Methods CSF samples were obtained from 11 consecutive patients. A beta 1-42, A beta 1-40, p-Tau, and t-Tau were quantified using the LUMIPULSE G600II automated platform. Results Temperature and storage days significantly influenced A beta 1-42 and A beta 1-40 with concentrations decreasing with days spent at 4 degrees C. The use of the A beta 1-42/A beta 1-40 ratio could partly compensate it. P-Tau and t-Tau were not affected by any of the tested storage conditions. For conditions involving storage at 4 degrees C, a correction factor of 1.081 can be applied. Diagnostic agreement was almost perfect in all conditions. Conclusions Cutoffs calculated in samples stored at -80 degrees C can be safely used in samples stored at -20 degrees C for 15-16 days or up to two days at RT and subsequent freezing at -80 degrees C. For samples stored at 4 degrees C, cutoffs would require applying a correction factor, allowing to work with the certainty of reaching the same clinical diagnosis.
Filiaciones:
Ferrer, R:
Hosp La Santa Creu & St Pau, Dept Biochem, Biomed Res Inst IIB St Pau, Barcelona, Spain
Zhu, N:
Hosp Santa Creu & Sant Pau, Dept Neurol, St Pau Memory Unit, Biomed Res Inst IIB St Pau, C St Quinti 89, Barcelona 08041, Spain
Arranz, J:
Hosp Santa Creu & Sant Pau, Dept Neurol, St Pau Memory Unit, Biomed Res Inst IIB St Pau, C St Quinti 89, Barcelona 08041, Spain
Porcel, I:
Hosp La Santa Creu & St Pau, Dept Biochem, Biomed Res Inst IIB St Pau, Barcelona, Spain
El Bounasri, S:
Hosp Santa Creu & Sant Pau, Dept Neurol, St Pau Memory Unit, Biomed Res Inst IIB St Pau, C St Quinti 89, Barcelona 08041, Spain
Sanchez, O:
Hosp Santa Creu & Sant Pau, Dept Neurol, St Pau Memory Unit, Biomed Res Inst IIB St Pau, C St Quinti 89, Barcelona 08041, Spain
Torres, S:
Hosp Santa Creu & Sant Pau, Dept Neurol, St Pau Memory Unit, Biomed Res Inst IIB St Pau, C St Quinti 89, Barcelona 08041, Spain
Julve, J:
Ctr Biomed Invest Network Diabet & Metab Dis CIBE, Madrid, Spain
Lleo, A:
Hosp Santa Creu & Sant Pau, Dept Neurol, St Pau Memory Unit, Biomed Res Inst IIB St Pau, C St Quinti 89, Barcelona 08041, Spain
Ctr Biomed Invest Network Neurodegenerat Dis CIBE, Madrid, Spain
Blanco-Vaca, F:
Hosp La Santa Creu & St Pau, Dept Biochem, Biomed Res Inst IIB St Pau, Barcelona, Spain
Ctr Biomed Invest Network Diabet & Metab Dis CIBE, Madrid, Spain
Univ Autonoma Barcelona, Dept Biochem & Mol Biol, Barcelona, Spain
Alcolea, D:
Hosp Santa Creu & Sant Pau, Dept Neurol, St Pau Memory Unit, Biomed Res Inst IIB St Pau, C St Quinti 89, Barcelona 08041, Spain
Ctr Biomed Invest Network Neurodegenerat Dis CIBE, Madrid, Spain
Tondo, M:
Hosp La Santa Creu & St Pau, Dept Biochem, Biomed Res Inst IIB St Pau, Barcelona, Spain
Ctr Biomed Invest Network Diabet & Metab Dis CIBE, Madrid, Spain
Soc Espanola Med Lab, Comis Neuroquim & Enfermedades Neurol, Barcelona, Spain
Open Access
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