Mobile Genetic Elements Related to the Diffusion of Plasmid-Mediated AmpC beta-Lactamases or Carbapenemases from Enterobacteriaceae: Findings from a Multicenter Study in Spain
Por:
Zamorano, L, Miro, E, Juan, C, Gomez, L, Bou, G, Gonzalez-Lopez, JJ, Martinez-Martinez, L, Aracil, B, Conejo, MC, Oliver, A, Navarro, F
Publicada:
1 sep 2015
Resumen:
We examined the genetic context of 74 acquired ampC genes and 17 carbapenemase genes from 85 of 640 Enterobacteriaceae isolates collected in 2009. Using S1 pulsed-field gel electrophoresis and Southern hybridization, 37 of 74 bla(AmpC) genes were located on large plasmids of different sizes belonging to six incompatibility groups. We used sequencing and PCR mapping to investigate the regions flanking the acquired ampC genes. The bla(CMY-2)-like genes were associated with ISEcp1; the surrounding bla(DHA) genes were similar to Klebsiella pneumoniae plasmid pTN60013 associated with IS26 and the psp and sap operons; and the bla(ACC-1) genes were associated with IS26 elements inserted into ISEcp1. All of the carbapenemase genes (bla(VIM-1), bla(IMP-22), and bla(IMP-28)) were located in class 1 integrons. Therefore, although plasmids are the main cause of the rapid dissemination of ampC genes among Enterobacteriaceae, we need to be aware that other mobile genetic elements, such as insertion sequences, transposons, or integrons, can be involved in the mobilization of these genes of chromosomal origin. Additionally, three new integrons (In846 to In848) are described in this study.
Filiaciones:
Zamorano, L:
Hosp Univ Son Espases, Inst Invest Sanitaria Palma IdISPa, Microbiol Serv, Palma De Mallorca, Spain
Hosp Univ Son Espases, Inst Invest Sanitaria Palma IdISPa, Unidad Invest, Palma De Mallorca, Spain
Miro, E:
Hosp Santa Creu & Sant Pau, Inst Invest Biomed St Pau, Microbiol Serv, Barcelona, Spain
Juan, C:
Hosp Univ Son Espases, Inst Invest Sanitaria Palma IdISPa, Microbiol Serv, Palma De Mallorca, Spain
Hosp Univ Son Espases, Inst Invest Sanitaria Palma IdISPa, Unidad Invest, Palma De Mallorca, Spain
Gomez, L:
Hosp Santa Creu & Sant Pau, Inst Invest Biomed St Pau, Microbiol Serv, Barcelona, Spain
Bou, G:
Complejo Hosp Univ A Coruna, Microbiol Serv, La Coruna, Spain
Gonzalez-Lopez, JJ:
Hosp Valle De Hebron, Microbiol Serv, Barcelona, Spain
Univ Autonoma Barcelona, Dept Genet & Microbiol, E-08193 Barcelona, Spain
Martinez-Martinez, L:
Hosp Univ Marques de Valdecilla IDIVAL, Microbiol Serv, Cantabria, Spain
Univ Cantabria, Dept Biol Mol, Cantabria, Spain
Aracil, B:
Inst Salud Carlos III, Ctr Nacl Microbiol, Serv Bacteriol, Lab Antibiot, Madrid, Spain
Conejo, MC:
Univ Seville, Fac Med, Dept Microbiol, Seville, Spain
Oliver, A:
Hosp Univ Son Espases, Inst Invest Sanitaria Palma IdISPa, Microbiol Serv, Palma De Mallorca, Spain
Hosp Univ Son Espases, Inst Invest Sanitaria Palma IdISPa, Unidad Invest, Palma De Mallorca, Spain
Navarro, F:
Hosp Santa Creu & Sant Pau, Inst Invest Biomed St Pau, Microbiol Serv, Barcelona, Spain
Univ Autonoma Barcelona, Dept Genet & Microbiol, E-08193 Barcelona, Spain
Green Published, Bronze, Green Accepted
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