Viral Culture Confirmed SARS-CoV-2 Subgenomic RNA Value as a Good Surrogate Marker of Infectivity
Por:
Bravo, MS, Berengua, C, Marin, P, Esteban, M, Rodriguez, C, del Cuerpo, M, Miro, E, Cuesta, G, Mosquera, M, Sanchez-Palomino, S, Vila, J, Rabella, N, Marcos, MA
Publicada:
1 ene 2022
Resumen:
Determining SARS-CoV-2 viral infectivity is crucial for patient clinical assessment and isolation decisions. We assessed subgenomic RNA (sgRNA) as a surrogate marker of SARS-CoV-2 infectivity in SARS-CoV-2-positive reverse transcription PCR (RT-PCR) respiratory samples (n = 105) in comparison with viral culture as the reference standard for virus replication. sgRNA and viral isolation results were concordant in 99/105 cases (94%), indicating highly significant agreement between the two techniques (Cohen's kappa coefficient 0.88, 95% confidence interval [CI] 0.78 to 0.97, P < 0.001). sgRNA RT-PCR showed a sensitivity of 97% and a positive predictive value of 94% to detect replication-competent virus, further supporting sgRNA as a surrogate marker of SARS-CoV-2 infectivity. sgRNA RT-PCR is an accurate, rapid, and affordable technique that can overcome culture and cycle threshold (C-T) value limitations and be routinely implemented in hospital laboratories to detect viral infectivity, which is essential for optimizing patient monitoring, the efficacy of treatments/vaccines, and work reincorporation policies, as well as for safely shortening isolation precautions.
Filiaciones:
Bravo, MS:
Hosp Clin Barcelona, Inst Global Hlth Barcelona ISGlobal, Microbiol Dept, Barcelona, Spain
Berengua, C:
Univ Autonoma Barcelona, Microbiol Dept, Hosp Santa Creu & St Pau, Barcelona, Spain
Marin, P:
Univ Autonoma Barcelona, Microbiol Dept, Hosp Santa Creu & St Pau, Barcelona, Spain
Esteban, M:
Univ Autonoma Barcelona, Microbiol Dept, Hosp Santa Creu & St Pau, Barcelona, Spain
Rodriguez, C:
Hosp Clin Barcelona, Inst Global Hlth Barcelona ISGlobal, Microbiol Dept, Barcelona, Spain
del Cuerpo, M:
Univ Autonoma Barcelona, Microbiol Dept, Hosp Santa Creu & St Pau, Barcelona, Spain
Miro, E:
Univ Autonoma Barcelona, Microbiol Dept, Hosp Santa Creu & St Pau, Barcelona, Spain
Cuesta, G:
Hosp Clin Barcelona, Inst Global Hlth Barcelona ISGlobal, Microbiol Dept, Barcelona, Spain
Mosquera, M:
Hosp Clin Barcelona, Inst Global Hlth Barcelona ISGlobal, Microbiol Dept, Barcelona, Spain
Sanchez-Palomino, S:
Univ Barcelona, Hosp Clin, Inst Invest Biomed August Pi & Sunyer IDIBAPS, AIDS Res Grp, Barcelona, Spain
Vila, J:
Hosp Clin Barcelona, Inst Global Hlth Barcelona ISGlobal, Microbiol Dept, Barcelona, Spain
Rabella, N:
Univ Autonoma Barcelona, Microbiol Dept, Hosp Santa Creu & St Pau, Barcelona, Spain
Marcos, MA:
Hosp Clin Barcelona, Inst Global Hlth Barcelona ISGlobal, Microbiol Dept, Barcelona, Spain
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