Detection of azole resistance in Aspergillus fumigatus complex isolates MALDI-TOF mass
Por:
Zvezdanova, ME, Arroyo, MJ, Mendez, G, Candela, A, Mancera, L, Rodriguez, JG, Serra, JL, Jimenez, R, Lozano, I, Castro, C, Lopez, C, Munoz, P, Guinea, J, Escribano, P, Rodriguez-Sanchez, B, Sánchez-Reus F., ASPEIN Grp
Publicada:
1 feb 2022
Ahead of Print:
1 ene 2022
Resumen:
Objectives: The main goal of this study was to accurately detect azole resistance in species of the Aspergillus fumigatus complex by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Methods: Identification of isolates (n 1/4 868) was done with MALDI-TOF MS using both commercial and in-house libraries. To determine azole susceptibility, the EUCAST E.Def. 9.3.2 method was applied as the reference standard. Identification of resistant isolates was confirmed by DNA sequence analysis. Protein spectra obtained by MALDI-TOF MS were analysed to differentiate species within the A. fumigatus complex and to detect azole-resistant A. fumigatus sensu stricto isolates. Results: Correct discrimination of A. fumigatus sensu stricto from cryptic species was accomplished in 100% of the cases applying principal component analysis (PCA) to protein spectra generated by MALDI-TOF MS. Furthermore, a specific peak (4586 m/z) was found to be present only in cryptic species. The application of partial least squares (PLS) discriminant analysis allowed 98.43% (+/- 0.038) discrimination between susceptible and azole-resistant A. fumigatus sensu stricto isolates. Finally, based on PLS and SVM, A. fumigatus sensu stricto isolates with different cyp51A gene mutations were correctly clustered in 91.5% of the cases. Conclusions: MALDI-TOF MS combined with peak analysis is a novel tool that allows the differentiation of A. fumigatus sensu stricto from other species within the A. fumigatus complex, as well as the detection of azole-resistant A. fumigatus sensu stricto. Although further studies are still needed, the results reported here show the great potential of MALDI-TOF and machine learning for the rapid detection of azoleresistant Aspergillus fumigatus isolates from clinical origins. Margarita Estreya Zvezdanova, Clin Microbiol Infect 2022:28:260
Filiaciones:
Zvezdanova, ME:
Hosp Gen Univ Gregorio Maranon, Clin Microbiol & Infect Dis Dept, Madrid, Spain
Inst Invest Sanitaria Gregorio Maranon, Madrid, Spain
Arroyo, MJ:
Clover Bioanalyt Software, Av Innovac, Granada, Spain
Mendez, G:
Clover Bioanalyt Software, Av Innovac, Granada, Spain
Candela, A:
Hosp Gen Univ Gregorio Maranon, Clin Microbiol & Infect Dis Dept, Madrid, Spain
Inst Invest Sanitaria Gregorio Maranon, Madrid, Spain
Mancera, L:
Clover Bioanalyt Software, Av Innovac, Granada, Spain
Rodriguez, JG:
Hosp La Paz, Clin Microbiol Dept, Madrid, Spain
Serra, JL:
Hosp Gen Albacete, Clin Microbiol Dept, Albacete, Spain
Jimenez, R:
Complejo Hosp Toledo, Clin Microbiol Dept, Toledo, Spain
Lozano, I:
Hosp Univ Puerta Mar, Clin Microbiol Dept, Cadiz, Spain
Castro, C:
Hosp Valme, Clin Microbiol Dept, Seville, Spain
Lopez, C:
Hosp Univ Miguel Servet, Clin Microbiol Dept, Zaragoza, Spain
Univ Zaragoza, Inst Invest Sanitaria Aragon, Zaragoza, Spain
Munoz, P:
Hosp Gen Univ Gregorio Maranon, Clin Microbiol & Infect Dis Dept, Madrid, Spain
Inst Invest Sanitaria Gregorio Maranon, Madrid, Spain
CIBER Enfermedades Resp CIBERES CB06 06 0058, Madrid, Spain
Univ Complutense Madrid, Sch Med, Med Dept, Madrid, Spain
Guinea, J:
Hosp Gen Univ Gregorio Maranon, Clin Microbiol & Infect Dis Dept, Madrid, Spain
Inst Invest Sanitaria Gregorio Maranon, Madrid, Spain
CIBER Enfermedades Resp CIBERES CB06 06 0058, Madrid, Spain
Escribano, P:
Hosp Gen Univ Gregorio Maranon, Clin Microbiol & Infect Dis Dept, Madrid, Spain
Inst Invest Sanitaria Gregorio Maranon, Madrid, Spain
Rodriguez-Sanchez, B:
Hosp Gen Univ Gregorio Maranon, Clin Microbiol & Infect Dis Dept, Madrid, Spain
Inst Invest Sanitaria Gregorio Maranon, Madrid, Spain
Sánchez-Reus F.:
Institut d’Investigació Biomèdica Sant Pau (IIB SANT PAU), Sant Quintí 77-79, 08041 Barcelona, Spain
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