Efficacy of the FilmArray blood culture identification panel for direct molecular diagnosis of infectious diseases from samples other than blood
Por:
Mico, M, Navarro, F, de Miniac, D, Gonzalez, Y, Brell, A, Lopez, C, Sanchez-Reus, F, Mirelis, B, Coll, P
Publicada:
1 dic 2015
Resumen:
Molecular-based techniques reduce the delay in diagnosing infectious diseases and therefore contribute to better patient outcomes. We assessed the FilmArray blood culture identification (BCID) panel (Biofire Diagnostics/bioMerieux) directly on clinical specimens other than blood: cerebrospinal, joint, pleural and ascitic fluids, bronchoscopy samples and abscesses. We compared the results from 88 samples obtained by culture-based techniques. The percentage of agreement between the two methods was 75 % with a Cohen kappa value of 0.51. Global sensitivity and specificity using the FilmArray BCID panel were 71 and 97 %, respectively. Sensitivity was poorer in samples with a low bacterial load, such as ascitic and pleural fluids (25 %), whereas the sensitivity for abscess samples was high (89 %). These findings suggest that the FilmArray BCID panel could be useful to perform microbiological diagnosis directly from samples other than positive blood cultures, as it offers acceptable sensitivity and moderate agreement with conventional microbiological methods. Nevertheless, cost-benefit studies should be performed before introducing this method into algorithms for microbiological diagnostics.
Filiaciones:
Mico, M:
Hosp Santa Creu & St Pau Barcelona, Servei Microbiol, Barcelona, Spain
Navarro, F:
Hosp Santa Creu & St Pau Barcelona, Servei Microbiol, Barcelona, Spain
Inst Invest Biomed St Pau, Barcelona, Spain
Univ Autonoma Barcelona, E-08193 Barcelona, Spain
de Miniac, D:
Hosp Santa Creu & St Pau Barcelona, Servei Microbiol, Barcelona, Spain
Gonzalez, Y:
Hosp Santa Creu & St Pau Barcelona, Servei Microbiol, Barcelona, Spain
Brell, A:
Hosp Santa Creu & St Pau Barcelona, Servei Microbiol, Barcelona, Spain
Lopez, C:
Hosp Santa Creu & St Pau Barcelona, Servei Microbiol, Barcelona, Spain
Sanchez-Reus, F:
Hosp Santa Creu & St Pau Barcelona, Servei Microbiol, Barcelona, Spain
Inst Invest Biomed St Pau, Barcelona, Spain
Univ Autonoma Barcelona, E-08193 Barcelona, Spain
Mirelis, B:
Hosp Santa Creu & St Pau Barcelona, Servei Microbiol, Barcelona, Spain
Inst Invest Biomed St Pau, Barcelona, Spain
Univ Autonoma Barcelona, E-08193 Barcelona, Spain
Coll, P:
Hosp Santa Creu & St Pau Barcelona, Servei Microbiol, Barcelona, Spain
Inst Invest Biomed St Pau, Barcelona, Spain
Univ Autonoma Barcelona, E-08193 Barcelona, Spain
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