Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification
Por:
Rodriguez-Temporal, D, Alcaide, F, Marekovic, I, O'Connor, JA, Gorton, R, van Ingen, J, Van den Bossche, A, Hery-Arnaud, G, Beauruelle, C, Orth-Holler, D, Palacios-Gutierrez, JJ, Tudo, G, Bou, G, Ceyssens, PJ, Garrigo, M, Gonzalez-Martin, J, Greub, G, Hrabak, J, Ingebretsen, A, Mediavilla-Gradolph, MC, Oviano, M, Palop, B, Pranada, AB, Quiroga, L, Ruiz-Serrano, MJ, Rodriguez-Sanchez, B
Publicada:
24 ene 2022
Resumen:
The ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on the reproducibility of MALDI-TOF have been performed so far, none on mycobacteria. The aim of this study was to evaluate the reproducibility of MALDI-TOF for the identification of NTM in 15 laboratories in 9 European countries. A total of 98 NTM clinical isolates were grown on Lowenstein-Jensen. Biomass was collected in tubes with water and ethanol, anonymized and sent out to the 15 participating laboratories. Isolates were identified using MALDI Biotyper (Bruker Daltonics). Up to 1330 MALDI-TOF identifications were collected in the study. A score >= 1.6 was obtained for 100% of isolates in 5 laboratories (68.2-98.6% in the other). Species-level identification provided by MALDI-TOF was 100% correct in 8 centres and 100% correct to complex-level in 12 laboratories. In most cases, the misidentifications obtained were associated with closely related species. The variability observed for a few isolates could be due to variations in the protein extraction procedure or to MALDI-TOF system status in each centre. In conclusion, MALDI-TOF showed to be a highly reproducible method and suitable for its implementation for NTM identification.
Filiaciones:
Rodriguez-Temporal, D:
Hosp Gen Univ Gregorio Maranon, Dept Clin Microbiol & Infect Dis, Madrid, Spain
Alcaide, F:
Univ Barcelona, Serv Microbiol, Hosp Univ Bellvitge IDIBELL, Dept Patol & Terapeut Expt,Fac Med & Ciencies La, Lhospitalet De Llobregat, Spain
Marekovic, I:
Univ Hosp Ctr Zagreb, Dept Clin & Mol Microbiol, Zagreb, Croatia
O'Connor, JA:
Munster Technol Univ, Dept Biol Sci, Cork, Ireland
Gorton, R:
Hlth Serv Labs, London, England
van Ingen, J:
Radboud Univ Nijmegen Med Ctr, Nijmegen, Netherlands
Van den Bossche, A:
Sciensano, Div Human Bacterial Dis, Brussels, Belgium
Hery-Arnaud, G:
Hop Cavale Blanche, Plateforme Biol, Lab Bacteriol, Brest, France
Beauruelle, C:
Hop Cavale Blanche, Plateforme Biol, Lab Bacteriol, Brest, France
Orth-Holler, D:
Med Univ Innsbruck, Div Hyg & Med Microbiol, Innsbruck, Austria
Palacios-Gutierrez, JJ:
Hosp Univ Cent Asturias, Serv Microbiol, Oviedo, Spain
Tudo, G:
Univ Barcelona, Fac Med & Ciencies Salut, Serv Microbiol CDB, Hosp Clin Barcelona ISGlobal,Dept Fonaments Clin, Barcelona, Spain
Bou, G:
Hosp Univ A Coruna, Serv Microbiol, Coruna, Spain
Ceyssens, PJ:
Sciensano, Div Human Bacterial Dis, Brussels, Belgium
Garrigo, M:
Hosp Santa Creu & Sant Pau, Lab Microbiol, Barcelona, Spain
Gonzalez-Martin, J:
Univ Barcelona, Fac Med & Ciencies Salut, Serv Microbiol CDB, Hosp Clin Barcelona ISGlobal,Dept Fonaments Clin, Barcelona, Spain
Greub, G:
Univ Lausanne, Inst Microbiol, Lausanne, Switzerland
Hrabak, J:
Fac Med Plzen, Biomed Ctr, Plzen, Czech Republic
Ingebretsen, A:
Oslo Univ Hosp, Oslo, Norway
Mediavilla-Gradolph, MC:
Hosp Reg Malaga, Lab Microbiol, Malaga, Spain
Oviano, M:
Hosp Univ A Coruna, Serv Microbiol, Coruna, Spain
Palop, B:
Hosp Reg Malaga, Lab Microbiol, Malaga, Spain
Pranada, AB:
MVZ Dr Eberhard & Partner Dortmund, Dept Med Microbiol, Dortmund, Germany
Quiroga, L:
Hosp Gen Univ Gregorio Maranon, Dept Clin Microbiol & Infect Dis, Madrid, Spain
Ruiz-Serrano, MJ:
Hosp Gen Univ Gregorio Maranon, Dept Clin Microbiol & Infect Dis, Madrid, Spain
Rodriguez-Sanchez, B:
Hosp Gen Univ Gregorio Maranon, Dept Clin Microbiol & Infect Dis, Madrid, Spain
Green Published, gold, All Open Access, Gold, Green
|