Airway Mucin 2 Is Decreased in Patients with Severe Chronic Obstructive Pulmonary Disease with Bacterial Colonization
Por:
Sibila, O, Garcia-Bellmunt, L, Giner, J, Rodrigo-Troyano, A, Suarez-Cuartin, G, Torrego, A, Castillo, D, Solanes, I, Mateus, EF, Vidal, S, Sanchez-Reus, F, Sala, E, Cosio, BG, Restrepo, MI, Anzueto, A, Chalmers, JD, Plaza, V
Publicada:
1 may 2016
Resumen:
Rationale: Mucins are essential for airway defense against bacteria. We hypothesized that abnormal secreted airway mucin levels would be associated with bacterial colonization in patients with severe chronic obstructive pulmonary disease (COPD)
Objectives: To investigate the relationship between mucin levels and the presence of potentially pathogenic micro-organisms in the airways of stable patients with severe COPD
Methods: Clinically stable patients with severe COPD were examined prospectively. All patients underwent a computerized tomography scan, lung function tests, induced sputum collection, and bronchoscopy with bronchoalveolar lavage (BAL) and protected specimen brush. Patients with bronchiectasis were excluded. Secreted mucins (MUC2, MUC5AC, and MUC5B) and inflammatory markers were assessed in BAL and sputum by ELISA.
Measurements and Main Results: We enrolled 45 patients, with mean age (+/- SD) of 67 (68) years and mean FEV1 of 41 (+/- 10) % predicted. A total of 31% (n = 14) of patients had potentially pathogenic micro-organisms in quantitative bacterial cultures of samples obtained by protected specimen brush. Patients with COPD with positive cultures had lower levels of MUC2 both in BAL (P = 0.02) and in sputum (P = 0.01). No differences in MUC5B or MUC5AC levels were observed among the groups. Lower MUC2 levels were correlated with lower FEV1 (r = 0.32, P = 0.04) and higher sputum IL-6 (r = 20.40, P = 0.01).
Conclusions: Airway MUC2 levels are decreased in patients with severe COPD colonized by potentially pathogenic micro-organisms. These findings may indicate one of the mechanisms underlying airway colonization in patients with severe COPD.
Filiaciones:
Sibila, O:
Univ Autonoma Barcelona, Hosp Santa Creu & St Pau, Serv Pneumol, Barcelona, Spain
Inst Invest Biomed St Pau, Barcelona, Spain
Garcia-Bellmunt, L:
Univ Autonoma Barcelona, Hosp Santa Creu & St Pau, Serv Pneumol, Barcelona, Spain
Inst Invest Biomed St Pau, Barcelona, Spain
Giner, J:
Univ Autonoma Barcelona, Hosp Santa Creu & St Pau, Serv Pneumol, Barcelona, Spain
Inst Invest Biomed St Pau, Barcelona, Spain
Rodrigo-Troyano, A:
Univ Autonoma Barcelona, Hosp Santa Creu & St Pau, Serv Pneumol, Barcelona, Spain
Inst Invest Biomed St Pau, Barcelona, Spain
Suarez-Cuartin, G:
Univ Autonoma Barcelona, Hosp Santa Creu & St Pau, Serv Pneumol, Barcelona, Spain
Inst Invest Biomed St Pau, Barcelona, Spain
Torrego, A:
Univ Autonoma Barcelona, Hosp Santa Creu & St Pau, Serv Pneumol, Barcelona, Spain
Inst Invest Biomed St Pau, Barcelona, Spain
Castillo, D:
Univ Autonoma Barcelona, Hosp Santa Creu & St Pau, Serv Pneumol, Barcelona, Spain
Inst Invest Biomed St Pau, Barcelona, Spain
Solanes, I:
Univ Autonoma Barcelona, Hosp Santa Creu & St Pau, Serv Pneumol, Barcelona, Spain
Inst Invest Biomed St Pau, Barcelona, Spain
Mateus, EF:
Inst Invest Biomed St Pau, Barcelona, Spain
Univ Autonoma Barcelona, Hosp Santa Creu & St Pau, Lab Immunol Expt, Barcelona, Spain
Vidal, S:
Inst Invest Biomed St Pau, Barcelona, Spain
Univ Autonoma Barcelona, Hosp Santa Creu & St Pau, Lab Immunol Expt, Barcelona, Spain
Sanchez-Reus, F:
Inst Invest Biomed St Pau, Barcelona, Spain
Univ Autonoma Barcelona, Hosp Santa Creu & St Pau, Serv Microbiol, Barcelona, Spain
Sala, E:
Hosp Son Espases, Inst Invest Sanitaria Palma, Serv Pneumol, Palma de Mallorca, Spain
CIBER Enfermedades Resp, Madrid, Spain
Cosio, BG:
Hosp Son Espases, Inst Invest Sanitaria Palma, Serv Pneumol, Palma de Mallorca, Spain
CIBER Enfermedades Resp, Madrid, Spain
Restrepo, MI:
South Texas Vet Hlth Care Syst, San Antonio, TX USA
Univ Texas Hlth Sci Ctr San Antonio, San Antonio, TX 78229 USA
Anzueto, A:
South Texas Vet Hlth Care Syst, San Antonio, TX USA
Univ Texas Hlth Sci Ctr San Antonio, San Antonio, TX 78229 USA
Chalmers, JD:
Univ Dundee, Tayside Resp Res Grp, Dundee, Scotland
Plaza, V:
Univ Autonoma Barcelona, Hosp Santa Creu & St Pau, Serv Pneumol, Barcelona, Spain
Inst Invest Biomed St Pau, Barcelona, Spain
Green Published
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