Cartilage resurfacing potential of PLGA scaffolds loaded with autologous cells from cartilage, fat, and bone marrow in an ovine model of osteochondral focal defect
Por:
Caminal, M, Peris, D, Fonseca, C, Barrachina, J, Codina, D, Rabanal, RM, Moll, X, Morist, A, Garcia, F, Cairo, JJ, Godia, F, Pla, A, Vives, J
Publicada:
1 ago 2016
Resumen:
Current developments in tissue engineering strategies for articular cartilage regeneration focus on the design of supportive three-dimensional scaffolds and their use in combination with cells from different sources. The challenge of translating initial successes in small laboratory animals into the clinics involves pilot studies in large animal models, where safety and efficacy should be investigated during prolonged follow-up periods. Here we present, in a single study, the long-term (up to 1 year) effect of biocompatible porous scaffolds non-seeded and seeded with fresh ex vivo expanded autologous progenitor cells that were derived from three different cell sources [cartilage, fat and bone marrow (BM)] in order to evaluate their advantages as cartilage resurfacing agents. An ovine model of critical size osteochondral focal defect was used and the test items were implanted arthroscopically into the knees. Evidence of regeneration of hyaline quality tissue was observed at 6 and 12 months post-treatment with variable success depending on the cell source. Cartilage and BM-derived mesenchymal stromal cells (MSC), but not those derived from fat, resulted in the best quality of new cartilage, as judged qualitatively by magnetic resonance imaging and macroscopic assessment, and by histological quantitative scores. Given the limitations in sourcing cartilage tissue and the risk of donor site morbidity, BM emerges as a preferential source of MSC for novel cartilage resurfacing therapies of osteochondral defects using copolymeric poly-d,l-lactide-co-glycolide scaffolds.
Filiaciones:
Caminal, M:
Banc Sang & Teixits, Div Terapies Avancades XCELIA, Edifici Dr Frederic Duran & Jorda, Barcelona 08005, Spain
Peris, D:
Univ Autonoma Barcelona, Escola Engn, Dept Engn Quim, Grp Engn Cellular & Tissular, Edifici Q,Campus UAB, Bellaterra 08193, Cerdanyola Del, Spain
Fonseca, C:
Univ Autonoma Barcelona, Dept Med & Cirurgia Anim, Area Med & Cirurgia Anim, Edifici Q,Campus UAB, Bellaterra 08193, Cerdanyola Del, Spain
Barrachina, J:
Hosp ASEPEYO St Cugat, Avinguda Alcalde Barnils 54-60, Barcelona 08174, Spain
Codina, D:
Hosp ASEPEYO St Cugat, Avinguda Alcalde Barnils 54-60, Barcelona 08174, Spain
Rabanal, RM:
Univ Autonoma Barcelona, Dept Med & Cirurgia Anim, Area Med & Cirurgia Anim, Edifici Q,Campus UAB, Bellaterra 08193, Cerdanyola Del, Spain
Moll, X:
Univ Autonoma Barcelona, Dept Med & Cirurgia Anim, Area Med & Cirurgia Anim, Edifici Q,Campus UAB, Bellaterra 08193, Cerdanyola Del, Spain
Morist, A:
Univ Autonoma Barcelona, Dept Med & Cirurgia Anim, Area Med & Cirurgia Anim, Edifici Q,Campus UAB, Bellaterra 08193, Cerdanyola Del, Spain
Garcia, F:
Univ Autonoma Barcelona, Dept Med & Cirurgia Anim, Area Med & Cirurgia Anim, Edifici Q,Campus UAB, Bellaterra 08193, Cerdanyola Del, Spain
Cairo, JJ:
Univ Autonoma Barcelona, Escola Engn, Dept Engn Quim, Grp Engn Cellular & Tissular, Edifici Q,Campus UAB, Bellaterra 08193, Cerdanyola Del, Spain
Godia, F:
Univ Autonoma Barcelona, Escola Engn, Dept Engn Quim, Grp Engn Cellular & Tissular, Edifici Q,Campus UAB, Bellaterra 08193, Cerdanyola Del, Spain
Pla, A:
Banc Sang & Teixits, Div Terapies Avancades XCELIA, Edifici Dr Frederic Duran & Jorda, Barcelona 08005, Spain
Vives, J:
Banc Sang & Teixits, Div Terapies Avancades XCELIA, Edifici Dr Frederic Duran & Jorda, Barcelona 08005, Spain
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