Deciphering the Resistome of the Widespread Pseudomonas aeruginosa Sequence Type 175 International High-Risk Clone through Whole-Genome Sequencing
Por:
Cabot, G, Lopez-Causape, C, Ocampo-Sosa, AA, Sommer, LM, Dominguez, MA, Zamorano, L, Juan, C, Tubau, F, Rodriguez, C, Moya, B, Pena, C, Martinez-Martinez, L, Plesiat, P, Oliver, A, Pomar V., Gurgui M.
Publicada:
1 dic 2016
Resumen:
Whole-genome sequencing (WGS) was used for the characterization of the frequently extensively drug resistant (XDR) Pseudomonas aeruginosa sequence type 175 (ST175) high-risk clone. A total of 18 ST175 isolates recovered from 8 different Spanish hospitals were analyzed; 4 isolates from 4 different French hospitals were included for comparison. The typical resistance profile of ST175 included penicillins, cephalosporins, monobactams, carbapenems, aminoglycosides, and fluoroquinolones. In the phylogenetic analysis, the four French isolates clustered together with two isolates from one of the Spanish regions. Sequence variation was analyzed for 146 chromosomal genes related to antimicrobial resistance, and horizontally acquired genes were explored using online databases. The resistome of ST175 was determined mainly by mutational events; resistance traits common to all or nearly all of the strains included specific ampR mutations leading to ampC overexpression, specific mutations in oprD conferring carbapenem resistance, or a mexZ mutation leading to MexXY overexpression. All isolates additionally harbored an aadB gene conferring gentamicin and tobramycin resistance. Several other resistance traits were specific to certain geographic areas, such as a streptomycin resistance gene, aadA13, detected in all four isolates from France and in the two isolates from the Cantabria region and a glpT mutation conferring fosfomycin resistance, detected in all but these six isolates. Finally, several unique resistance mutations were detected in single isolates; particularly interesting were those in genes encoding penicillin-binding proteins (PBP1A, PBP3, and PBP4). Thus, these results provide information valuable for understanding the genetic basis of resistance and the dynamics of the dissemination and evolution of high-risk clones.
Filiaciones:
Cabot, G:
Hosp Son Espases, Inst Invest Sanitaria Palma IdISPa, Microbiol Serv, Palma De Mallorca, Spain
Hosp Son Espases, Inst Invest Sanitaria Palma IdISPa, Unidad Invest, Palma De Mallorca, Spain
Lopez-Causape, C:
Hosp Son Espases, Inst Invest Sanitaria Palma IdISPa, Microbiol Serv, Palma De Mallorca, Spain
Hosp Son Espases, Inst Invest Sanitaria Palma IdISPa, Unidad Invest, Palma De Mallorca, Spain
Ocampo-Sosa, AA:
Hosp Marques Valdecilla, Inst Invest Marques Valdecilla, Microbiol Serv, Santander, Spain
Sommer, LM:
Tech Univ Denmark, Novo Nordisk Fdn, Ctr Biosustainabil, Horsholm, Denmark
Dominguez, MA:
Bellvitge Hosp, Microbiol Serv, Barcelona, Spain
Inst Salud Carlos III, CIBERES, Madrid, Spain
Zamorano, L:
Hosp Son Espases, Inst Invest Sanitaria Palma IdISPa, Microbiol Serv, Palma De Mallorca, Spain
Hosp Son Espases, Inst Invest Sanitaria Palma IdISPa, Unidad Invest, Palma De Mallorca, Spain
Juan, C:
Hosp Son Espases, Inst Invest Sanitaria Palma IdISPa, Microbiol Serv, Palma De Mallorca, Spain
Hosp Son Espases, Inst Invest Sanitaria Palma IdISPa, Unidad Invest, Palma De Mallorca, Spain
Tubau, F:
Bellvitge Hosp, Microbiol Serv, Barcelona, Spain
Inst Salud Carlos III, CIBERES, Madrid, Spain
Rodriguez, C:
Hosp Marques Valdecilla, Inst Invest Marques Valdecilla, Microbiol Serv, Santander, Spain
Moya, B:
Hosp Son Espases, Inst Invest Sanitaria Palma IdISPa, Microbiol Serv, Palma De Mallorca, Spain
Hosp Son Espases, Inst Invest Sanitaria Palma IdISPa, Unidad Invest, Palma De Mallorca, Spain
Pena, C:
Hosp Virgen Los Lirios, Med Interna Serv, Alcoy, Spain
Martinez-Martinez, L:
Hosp Marques Valdecilla, Inst Invest Marques Valdecilla, Microbiol Serv, Santander, Spain
Univ Cantabria, Dept Biol Mol, Santander, Spain
Plesiat, P:
Univ Hosp Besancon, French Natl Reference Ctr Antibiot Resistance, Besancon, France
Oliver, A:
Hosp Son Espases, Inst Invest Sanitaria Palma IdISPa, Microbiol Serv, Palma De Mallorca, Spain
Hosp Son Espases, Inst Invest Sanitaria Palma IdISPa, Unidad Invest, Palma De Mallorca, Spain
Pomar V.:
Hospital Universitari de la Santa Creu i Sant Pau, Barcelona, Spain
Gurgui M.:
Hospital Universitari de la Santa Creu i Sant Pau, Barcelona, Spain
Bronze, Green Published
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