Adipocyte Lipopolysaccharide Binding Protein (LBP) is Linked to a Specific Lipidomic Signature
Por:
Moreno-Navarrete, JM, Jove, M, Padro, T, Boada, J, Ortega, F, Ricart, W, Pamplona, R, Badimon, L, Portero-Otin, M, Fernandez-Real, JM
Publicada:
1 feb 2017
Resumen:
Objective: Lipopolysaccharide binding protein ( LBP) is part of a family of structurally and functionally related lipid transfer proteins. This study aimed to investigate the associations of LBP with the lipid composition of human adipose tissue.
Methods: Lipidomic analysis was performed in whole adipose tissue. To validate the associations found, LBP was knocked down ( KD) in 3T3-L1 adipocytes, and lipidomic profile was evaluated by nontargeted lipidomics.
Results: LBP gene expression was negatively associated with phosphatidylcholine, phosphatidylserine, and sphingomyelin relative abundance in vivo. LBP expression was also decreased in those samples with the highest docosahexanoic content, implicated in the resolution of inflammation. The KD of LBP ( by similar to 70%) led to sharp changes in the lipidome of adipocytes. Of note, specific plasmalogen species PE( O-16:0/22:5), PE(38: 2), odd chain glycerolipids, and cholesteryl linoleate were upregulated by LBP KD. In contrast, GM3 gangliosides, several ceramides, a triacylglycerol potentially containing arachidonate, and cholesteryl palmitate were downregulated by LBP KD.
Conclusions: LBP seems to play a role in the regulation of lipid composition of adipose tissue linked to resilience to inflammation.
Filiaciones:
Moreno-Navarrete, JM:
CIBEROBN CB06 03 010, Inst Invest Biomed Girona IdIBGi, Dept Diabet Endocrinol & Nutr, Girona, Spain
ISCIII, Girona, Spain
Jove, M:
PCiTAL IRBLLEIDA Univ Lleida, NUTREN Nutrigen, Lleida, Spain
Padro, T:
IIB St Pau, Hosp Santa Creu & St Pau, CSIC ICCC, Cardiovasc Res Ctr, Barcelona, Spain
Boada, J:
PCiTAL IRBLLEIDA Univ Lleida, NUTREN Nutrigen, Lleida, Spain
Ortega, F:
CIBEROBN CB06 03 010, Inst Invest Biomed Girona IdIBGi, Dept Diabet Endocrinol & Nutr, Girona, Spain
ISCIII, Girona, Spain
Ricart, W:
CIBEROBN CB06 03 010, Inst Invest Biomed Girona IdIBGi, Dept Diabet Endocrinol & Nutr, Girona, Spain
ISCIII, Girona, Spain
Pamplona, R:
PCiTAL IRBLLEIDA Univ Lleida, NUTREN Nutrigen, Lleida, Spain
Badimon, L:
IIB St Pau, Hosp Santa Creu & St Pau, CSIC ICCC, Cardiovasc Res Ctr, Barcelona, Spain
Portero-Otin, M:
PCiTAL IRBLLEIDA Univ Lleida, NUTREN Nutrigen, Lleida, Spain
Fernandez-Real, JM:
CIBEROBN CB06 03 010, Inst Invest Biomed Girona IdIBGi, Dept Diabet Endocrinol & Nutr, Girona, Spain
ISCIII, Girona, Spain
|