Identification of Trypanosoma cruzi Discrete Typing Units (DTUs) in Latin-American migrants in Barcelona (Spain)
Por:
Abras, A, Gallego, M, Munoz, C, Juiz, NA, Ramirez, JC, Cura, CI, Tebar, S, Fernandez-Arevalo, A, Pinazo, MJ, de la Torre, L, Posada, E, Navarro, F, Espinal, P, Ballart, C, Portus, M, Gascon, J, Schijman, AG
Publicada:
1 abr 2017
Resumen:
Trypanosoma cruzi, the causative agent of Chagas disease, is divided into six Discrete Typing Units (DTUs): TcI-TcVI. We aimed to identify T. cruzi DTUs in Latin-American migrants in the Barcelona area (Spain) and to assess different molecular typing approaches for the characterization of T. cruzi genotypes. Seventy-five peripheral blood samples were analyzed by two real-time PCR methods (qPCR) based on satellite DNA (SatDNA) and kinetoplastid DNA (kDNA). The 20 samples testing positive in both methods, all belonging to Bolivian individuals, were submitted to DTU characterization using two PCR-based flowcharts: multiplex qPCR using TaqMan probes (MTq-PCR), and conventional PCR. These samples were also studied by sequencing the SatDNA and classified as type I (TcI/III), type II (TclI/IV) and type I/II hybrid (TcV/VI). Ten out of the 20 samples gave positive results in the flowcharts: TcV (5 samples), TcII/V/VI (3) and mixed infections by TcV plus TclI (1) and TcV plus Tcll/VI (1). By SatDNA sequencing, we classified the 20 samples, 19 as type I/II and one as type I. The most frequent DTU identified by both flowcharts, and suggested by SatDNA sequencing in the remaining samples with low parasitic loads, TcV, is common in Bolivia and predominant in peripheral blood. The mixed infection by TcV-TclI was detected for the first time simultaneously in Bolivian migrants. PCR-based flowcharts are very useful to characterize DTUs during acute infection. SatDNA sequence analysis cannot discriminate T. cruzi populations at the level of a single DTU but it enabled us to increase the number of characterized cases in chronically infected patients. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
Filiaciones:
Abras, A:
Univ Barcelona, Fac Farm, Dept Biol Sanitat Medi Ambient, Seccio Parasitol, E-08028 Barcelona, Spain
ISGlobal, Barcelona Ctr Int Hlth Res CRESIB, Rosello 134-4, Barcelona 08036, Spain
Univ Girona, Dept Biol, Lab Ictiol Genet, Girona 17071, Spain
Gallego, M:
Univ Barcelona, Fac Farm, Dept Biol Sanitat Medi Ambient, Seccio Parasitol, E-08028 Barcelona, Spain
ISGlobal, Barcelona Ctr Int Hlth Res CRESIB, Rosello 134-4, Barcelona 08036, Spain
Munoz, C:
Hosp Santa Creu & Sant Pau, Serv Microbiol, Sant Quinti 89, Barcelona 08041, Spain
Hosp Santa Creu & Sant Pau, IIB Sant Pau, Antoni Maria Claret 167, Barcelona 08025, Spain
Univ Autonoma Barcelona, Dept Genet & Microbiol, Campus Bellaterra, Cerdanyola Del Valles 08193, Spain
Juiz, NA:
Consejo Nacl Invest Cient & Tecn, INGEBI, Inst Invest Ingn Genet Biol Mol Dr Hecto N Torres, Lab Biol Mol Enfermedad Chagas LaBMECh, Vuelta Obligado 2490-2 C1428ADN Ciudad Autonoma, Buenos Aires, DF, Argentina
Ramirez, JC:
Consejo Nacl Invest Cient & Tecn, INGEBI, Inst Invest Ingn Genet Biol Mol Dr Hecto N Torres, Lab Biol Mol Enfermedad Chagas LaBMECh, Vuelta Obligado 2490-2 C1428ADN Ciudad Autonoma, Buenos Aires, DF, Argentina
Cura, CI:
Consejo Nacl Invest Cient & Tecn, INGEBI, Inst Invest Ingn Genet Biol Mol Dr Hecto N Torres, Lab Biol Mol Enfermedad Chagas LaBMECh, Vuelta Obligado 2490-2 C1428ADN Ciudad Autonoma, Buenos Aires, DF, Argentina
Tebar, S:
Univ Barcelona, Fac Farm, Dept Biol Sanitat Medi Ambient, Seccio Parasitol, E-08028 Barcelona, Spain
ISGlobal, Barcelona Ctr Int Hlth Res CRESIB, Rosello 134-4, Barcelona 08036, Spain
Fernandez-Arevalo, A:
Univ Barcelona, Fac Farm, Dept Biol Sanitat Medi Ambient, Seccio Parasitol, E-08028 Barcelona, Spain
ISGlobal, Barcelona Ctr Int Hlth Res CRESIB, Rosello 134-4, Barcelona 08036, Spain
Hosp Santa Creu & Sant Pau, IIB Sant Pau, Antoni Maria Claret 167, Barcelona 08025, Spain
Pinazo, MJ:
ISGlobal, Barcelona Ctr Int Hlth Res CRESIB, Rosello 134-4, Barcelona 08036, Spain
de la Torre, L:
ISGlobal, Barcelona Ctr Int Hlth Res CRESIB, Rosello 134-4, Barcelona 08036, Spain
Posada, E:
ISGlobal, Barcelona Ctr Int Hlth Res CRESIB, Rosello 134-4, Barcelona 08036, Spain
Navarro, F:
Hosp Santa Creu & Sant Pau, Serv Microbiol, Sant Quinti 89, Barcelona 08041, Spain
Hosp Santa Creu & Sant Pau, IIB Sant Pau, Antoni Maria Claret 167, Barcelona 08025, Spain
Univ Autonoma Barcelona, Dept Genet & Microbiol, Campus Bellaterra, Cerdanyola Del Valles 08193, Spain
Espinal, P:
Hosp Santa Creu & Sant Pau, IIB Sant Pau, Antoni Maria Claret 167, Barcelona 08025, Spain
Ballart, C:
Univ Barcelona, Fac Farm, Dept Biol Sanitat Medi Ambient, Seccio Parasitol, E-08028 Barcelona, Spain
ISGlobal, Barcelona Ctr Int Hlth Res CRESIB, Rosello 134-4, Barcelona 08036, Spain
Portus, M:
Univ Barcelona, Fac Farm, Dept Biol Sanitat Medi Ambient, Seccio Parasitol, E-08028 Barcelona, Spain
Gascon, J:
ISGlobal, Barcelona Ctr Int Hlth Res CRESIB, Rosello 134-4, Barcelona 08036, Spain
Schijman, AG:
Consejo Nacl Invest Cient & Tecn, INGEBI, Inst Invest Ingn Genet Biol Mol Dr Hecto N Torres, Lab Biol Mol Enfermedad Chagas LaBMECh, Vuelta Obligado 2490-2 C1428ADN Ciudad Autonoma, Buenos Aires, DF, Argentina
Open Access
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