Markers of endothelial damage in patients with chronic kidney disease on hemodialysis
Por:
Carmona, A, Aguera, ML, Luna-Ruiz, C, Buendia, P, Calleros, L, Garcia-Jerez, A, Rodriguez-Puyol, M, Arias, M, Arias-Guillen, M, de Arriba, G, Ballarin, J, Bernis, C, Fernandez, E, Garcia-Rebollo, S, Mancha, J, del Peso, G, Perez, E, Poch, E, Portoles, JM, Rodriguez-Puyol, D, Sanchez-Villanueva, R, Sarro, F, Torres, A, Martin-Malo, A, Aljama, P, Ramirez, R, Carracedo, J
Publicada:
1 abr 2017
Resumen:
Patients with Stage 5 chronic kidney disease who are on hemodialysis (HD) remain in a chronic inflammatory state, characterized by the accumulation of uremic toxins that induce endothelial damage and cardiovascular disease (CVD). Our aim was to examine microvesicles (MVs), monocyte subpopulations, and angiopoietins (Ang) to identify prognostic markers in HD patients with or without diabetes mellitus (DM). A total of 160 prevalent HD patients from 10 centers across Spain were obtained from the Biobank of the Nephrology Renal Network (Madrid, Spain): 80 patients with DM and 80 patients without DM who were matched for clinical and demographic criteria. MVs from plasma and several monocyte subpopulations (CD14(2+)/CD16(+), CD14(+)/CD16(2+)) were analyzed by flow cytometry, and the plasma concentrations of Ang1 and Ang2 were quantified by ELISA. Data on CVD were gathered over the 5.5 yr after these samples were obtained. MV level, monocyte subpopulations (CD14(+)/CD16(2+) and CD142(+)/CD16(+)), and Ang2-to-Ang1 ratios increased in HD patients with DM compared with non-DM patients. Moreover, MV level above the median (264 MVs/mu l) was associated independently with greater mortality. MVs, monocyte subpopulations, and Ang2-to-Ang1 ratio can be used as predictors for CVD. In addition, MV level has a potential predictive value in the prevention of CVD in HD patients. These parameters undergo more extensive changes in patients with DM.
Filiaciones:
Carmona, A:
IMIBIC, Cordoba, Spain
Inst Salud Carlos III, Red Espanola Invest Renal, Redes Temat Invest Cooperativa Salud, RD16 0009, Madrid, Spain
Aguera, ML:
IMIBIC, Cordoba, Spain
Hosp Univ Reina Sofia, Unidad Gest Clin Nefrol, Cordoba, Spain
Inst Salud Carlos III, Red Espanola Invest Renal, Redes Temat Invest Cooperativa Salud, RD16 0009, Madrid, Spain
Luna-Ruiz, C:
IMIBIC, Cordoba, Spain
Inst Salud Carlos III, Red Espanola Invest Renal, Redes Temat Invest Cooperativa Salud, RD16 0009, Madrid, Spain
Buendia, P:
IMIBIC, Cordoba, Spain
Inst Salud Carlos III, Red Espanola Invest Renal, Redes Temat Invest Cooperativa Salud, RD16 0009, Madrid, Spain
Calleros, L:
Inst Salud Carlos III, Red Espanola Invest Renal, Redes Temat Invest Cooperativa Salud, RD16 0009, Madrid, Spain
Univ Alcala de Henares, Biol Sistemas Dept, Madrid, Spain
Inst Salud Carlos III, Biobanco Redes Temat Invest Cooperativa & Salud R, Madrid, Spain
Garcia-Jerez, A:
Inst Salud Carlos III, Red Espanola Invest Renal, Redes Temat Invest Cooperativa Salud, RD16 0009, Madrid, Spain
Univ Alcala de Henares, Biol Sistemas Dept, Madrid, Spain
Inst Salud Carlos III, Biobanco Redes Temat Invest Cooperativa & Salud R, Madrid, Spain
Rodriguez-Puyol, M:
Inst Salud Carlos III, Red Espanola Invest Renal, Redes Temat Invest Cooperativa Salud, RD16 0009, Madrid, Spain
Univ Alcala de Henares, Biol Sistemas Dept, Madrid, Spain
Inst Salud Carlos III, Biobanco Redes Temat Invest Cooperativa & Salud R, Madrid, Spain
Arias, M:
Inst Salud Carlos III, Red Espanola Invest Renal, Redes Temat Invest Cooperativa Salud, RD16 0009, Madrid, Spain
Hosp Univ Marques Valdecilla, Santander, Spain
Arias-Guillen, M:
Inst Salud Carlos III, Red Espanola Invest Renal, Redes Temat Invest Cooperativa Salud, RD16 0009, Madrid, Spain
Univ Barcelona, Hosp Clin Barcelona, Inst Invest Biomed August Pi I Sunyer, Dept Nefrol Trasplante Renal, Barcelona, Spain
de Arriba, G:
Inst Salud Carlos III, Red Espanola Invest Renal, Redes Temat Invest Cooperativa Salud, RD16 0009, Madrid, Spain
Hosp Univ Guadalajara, Guadalajara, Spain
Alcala Henares Univ, Dept Med & Especialidades Med, Madrid, Spain
Ballarin, J:
Inst Salud Carlos III, Red Espanola Invest Renal, Redes Temat Invest Cooperativa Salud, RD16 0009, Madrid, Spain
Fundacio Puigvert, Barcelona, Spain
Bernis, C:
Inst Salud Carlos III, Red Espanola Invest Renal, Redes Temat Invest Cooperativa Salud, RD16 0009, Madrid, Spain
Hosp Univ La Princesa Madrid, Madrid, Spain
Fernandez, E:
Inst Salud Carlos III, Red Espanola Invest Renal, Redes Temat Invest Cooperativa Salud, RD16 0009, Madrid, Spain
Hosp Univ Arnau Villanova Lleida, Lleida, Spain
Garcia-Rebollo, S:
Inst Salud Carlos III, Red Espanola Invest Renal, Redes Temat Invest Cooperativa Salud, RD16 0009, Madrid, Spain
Univ La Laguna, Hosp Univ Canarias, Ctr Invest Biomed Canarias, Serv Nefrol,Improving Biomed Res & Innovat Canary, San Cristobal la Laguna, Spain
Mancha, J:
Inst Salud Carlos III, Red Espanola Invest Renal, Redes Temat Invest Cooperativa Salud, RD16 0009, Madrid, Spain
Hosp Univ Principe Asturias, Madrid, Spain
del Peso, G:
Inst Salud Carlos III, Red Espanola Invest Renal, Redes Temat Invest Cooperativa Salud, RD16 0009, Madrid, Spain
Hosp Univ La Paz, Madrid, Spain
Perez, E:
Inst Salud Carlos III, Red Espanola Invest Renal, Redes Temat Invest Cooperativa Salud, RD16 0009, Madrid, Spain
Univ La Laguna, Hosp Univ Canarias, Ctr Invest Biomed Canarias, Serv Nefrol,Improving Biomed Res & Innovat Canary, San Cristobal la Laguna, Spain
Poch, E:
Inst Salud Carlos III, Red Espanola Invest Renal, Redes Temat Invest Cooperativa Salud, RD16 0009, Madrid, Spain
Univ Barcelona, Hosp Clin Barcelona, Inst Invest Biomed August Pi I Sunyer, Dept Nefrol Trasplante Renal, Barcelona, Spain
Portoles, JM:
Inst Salud Carlos III, Red Espanola Invest Renal, Redes Temat Invest Cooperativa Salud, RD16 0009, Madrid, Spain
Hosp Puerta Hierro, Madrid, Spain
Rodriguez-Puyol, D:
Inst Salud Carlos III, Red Espanola Invest Renal, Redes Temat Invest Cooperativa Salud, RD16 0009, Madrid, Spain
Sanchez-Villanueva, R:
Inst Salud Carlos III, Red Espanola Invest Renal, Redes Temat Invest Cooperativa Salud, RD16 0009, Madrid, Spain
Hosp Univ La Paz, Madrid, Spain
Sarro, F:
Inst Salud Carlos III, Red Espanola Invest Renal, Redes Temat Invest Cooperativa Salud, RD16 0009, Madrid, Spain
Hosp Univ Arnau Villanova Lleida, Lleida, Spain
Torres, A:
Inst Salud Carlos III, Red Espanola Invest Renal, Redes Temat Invest Cooperativa Salud, RD16 0009, Madrid, Spain
Univ La Laguna, Hosp Univ Canarias, Ctr Invest Biomed Canarias, Serv Nefrol,Improving Biomed Res & Innovat Canary, San Cristobal la Laguna, Spain
Martin-Malo, A:
IMIBIC, Cordoba, Spain
Hosp Univ Reina Sofia, Unidad Gest Clin Nefrol, Cordoba, Spain
Univ Cordoba, Dept Med Dermatol & Otorrinolaringol, Cordoba, Spain
Inst Salud Carlos III, Red Espanola Invest Renal, Redes Temat Invest Cooperativa Salud, RD16 0009, Madrid, Spain
Aljama, P:
IMIBIC, Cordoba, Spain
Hosp Univ Reina Sofia, Unidad Gest Clin Nefrol, Cordoba, Spain
Univ Cordoba, Dept Med Dermatol & Otorrinolaringol, Cordoba, Spain
Inst Salud Carlos III, Red Espanola Invest Renal, Redes Temat Invest Cooperativa Salud, RD16 0009, Madrid, Spain
Ramirez, R:
Hosp Univ Reina Sofia, Unidad Gest Clin Nefrol, Cordoba, Spain
Inst Salud Carlos III, Red Espanola Invest Renal, Redes Temat Invest Cooperativa Salud, RD16 0009, Madrid, Spain
Univ Alcala de Henares, Biol Sistemas Dept, Madrid, Spain
Carracedo, J:
IMIBIC, Cordoba, Spain
Inst Salud Carlos III, Red Espanola Invest Renal, Redes Temat Invest Cooperativa Salud, RD16 0009, Madrid, Spain
Univ Complutense, Fac Biol, Dept Anim Physiol 2, Madrid, Spain
Green Accepted
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