Identification of Leishmania by MatrixAssisted Laser Desorption IonizationTime of Flight (MALDI-TOF) Mass Spectrometry Using a Free Web-Based Application and a Dedicated Mass-Spectral Library
Por:
Lachaud, L, Fernandez-Arevalo, A, Normand, AC, Lami, P, Nabet, C, Donnadieu, JL, Piarroux, M, Djenad, F, Cassagne, C, Ravel, C, Tebar, S, Llovet, T, Blanchet, D, Demar, M, Harrat, Z, Aoun, K, Bastien, P, Munoz, C, Gallego, M, Piarroux, R
Publicada:
1 oct 2017
Resumen:
Human leishmaniases are widespread diseases with different clinical forms caused by about 20 species within the Leishmania genus. Leishmania species identification is relevant for therapeutic management and prognosis, especially for cutaneous and mucocutaneous forms. Several methods are available to identify Leishmania species from culture, but they have not been standardized for the majority of the currently described species, with the exception of multilocus enzyme electrophoresis. Moreover, these techniques are expensive, time-consuming, and not available in all laboratories. Within the last decade, mass spectrometry (MS) has been adapted for the identification of microorganisms, including Leishmania. However, no commercial reference mass-spectral database is available. In this study, a reference mass-spectral library (MSL) for Leishmania isolates, accessible through a free Web-based application (mass-spectral identification [MSI]), was constructed and tested. It includes mass-spectral data for 33 different Leishmania species, including species that infect humans, animals, and phlebotomine vectors. Four laboratories on two continents evaluated the performance of MSI using 268 samples, 231 of which were Leishmania strains. All Leishmania strains, but one, were correctly identified at least to the complex level. A risk of species misidentification within the Leishmania donovani, L. guyanensis, and L. braziliensis complexes was observed, as previously reported for other techniques. The tested application was reliable, with identification results being comparable to those obtained with reference methods but with a more favorable cost-efficiency ratio. This free online identification system relies on a scalable database and can be implemented directly in users' computers.
Filiaciones:
Lachaud, L:
Univ Montpellier, CHU Montpellier, Ctr Natl Reference Leishmanioses, Dept Parasitol Mycol, Montpellier, France
Fernandez-Arevalo, A:
Hosp Santa Creu & Sant Pau, Serv Microbiol, Barcelona, Spain
Univ Barcelona, Fac Farm & Ciencies Alimentacio, Seccio Parasitol, Barcelona, Spain
Univ Barcelona, Hosp Clin, ISGlobal, Barcelona Ctr Int Hlth Res CRESIB, Barcelona, Spain
Normand, AC:
Univ Aix Marseille, CHU Timone, Lab Parasitol Mycol, Marseille, France
Lami, P:
Univ Montpellier, CHU Montpellier, Ctr Natl Reference Leishmanioses, Dept Parasitol Mycol, Montpellier, France
Nabet, C:
Univ Guyane, Lab Hosp Univ Parasito Mycol, EPaT Ecosyst Amazoniens & Pathol Trop, EA 3593,Lab Associe CNR Leishmania, Cayenne, French Guiana
Donnadieu, JL:
Assoc Passerelles, Montpellier, France
Piarroux, M:
Univ Aix Marseille, INSERM, UMR 912, IRD,AMU, Marseille, France
Djenad, F:
Univ Aix Marseille, CHU Timone, Lab Parasitol Mycol, Marseille, France
Cassagne, C:
Univ Aix Marseille, CHU Timone, Lab Parasitol Mycol, Marseille, France
Ravel, C:
Univ Montpellier, CHU Montpellier, Ctr Natl Reference Leishmanioses, Dept Parasitol Mycol, Montpellier, France
Tebar, S:
Univ Barcelona, Fac Farm & Ciencies Alimentacio, Seccio Parasitol, Barcelona, Spain
Llovet, T:
Hosp Santa Creu & Sant Pau, Serv Microbiol, Barcelona, Spain
Univ Autonoma Barcelona, Dept Genet & Microbiol, Cerdanyola Del Valles, Spain
Blanchet, D:
Univ Guyane, Lab Hosp Univ Parasito Mycol, EPaT Ecosyst Amazoniens & Pathol Trop, EA 3593,Lab Associe CNR Leishmania, Cayenne, French Guiana
Demar, M:
Univ Guyane, Lab Hosp Univ Parasito Mycol, EPaT Ecosyst Amazoniens & Pathol Trop, EA 3593,Lab Associe CNR Leishmania, Cayenne, French Guiana
Harrat, Z:
Inst Pasteur Algiers, Lab Ecoepidemiol Parasitaire & Genet Populat, Algiers, Algeria
Aoun, K:
Inst Pasteur Tunis, Lab Parasitol Med Biotechnol & Biomol, Tunis, Tunisia
Bastien, P:
Univ Montpellier, CHU Montpellier, Ctr Natl Reference Leishmanioses, Dept Parasitol Mycol, Montpellier, France
Munoz, C:
Hosp Santa Creu & Sant Pau, Serv Microbiol, Barcelona, Spain
Univ Autonoma Barcelona, Dept Genet & Microbiol, Cerdanyola Del Valles, Spain
Gallego, M:
Univ Barcelona, Fac Farm & Ciencies Alimentacio, Seccio Parasitol, Barcelona, Spain
Univ Barcelona, Hosp Clin, ISGlobal, Barcelona Ctr Int Hlth Res CRESIB, Barcelona, Spain
Piarroux, R:
Univ Aix Marseille, CHU Timone, Lab Parasitol Mycol, Marseille, France
Green Published, Bronze
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