Establishment and validation of surface model for biodosimetry based on gamma-H2AX foci detection
Por:
Lopez, JS, Pujol-Canadell, M, Puig, P, Ribas, M, Carrasco, P, Armengol, G, Barquinero, JF
Publicada:
2 ene 2022
Ahead of Print:
1 nov 2021
Resumen:
Introduction: In the event of a radiation accident detecting gamma-H2AX foci is being accepted as fast method for triage and dose assessment. However, due to their disappearance kinetics, published calibrations have been constructed at specific post-irradiation times.
Objectives: To develop a surface, or tridimensional, model to estimate doses at times not included in the calibration analysis, and to validate it.
Materials and methods: Calibration data was obtained irradiating peripheral mononucleated cells from one donor with radiation doses ranging from 0 to 3 Gy, and gamma-H2AX foci were detected microscopically using a semi-automatic method, at different post-irradiation times from 0.5 to 24 h. For validation, in addition to the above-mentioned donor, blood samples from another donor were also used. Validation was done within the range of doses and post-irradiation times used in the calibration.
Results: The calibration data clearly shows that at each analyzed time, the gamma-H2AX foci frequency increases as dose increases, and for each dose this frequency decreases with post-irradiation time. The gamma-H2AX foci nucleus distribution was clearly overdispersed, for this reason to obtain bidimensional and tridimensional dose-effect relationships no probability distribution was assumed, and linear and non-linear least squares weighted regression was used. In the two validation exercises for most evaluated samples, the 95% confidence limits of the estimated dose were between +/- 0.5 Gy of the real dose. No major differences were observed between donors.
Conclusion: In case of a suspected overexposure to radiation, the surface model here presented allows a correct dose estimation using gamma-H2AX foci as biomarker. The advantage of this surface model is that it can be used at any post-irradiation time, in our model between 0.5 and 24 h.
Filiaciones:
Lopez, JS:
Univ Autonoma Barcelona, Dept Biol Anim Biol Vegetal & Ecol, Unitat Antropol Biol, E-08193 Bellaterra, Catalonia, Spain
Pujol-Canadell, M:
Univ Autonoma Barcelona, Dept Biol Anim Biol Vegetal & Ecol, Unitat Antropol Biol, E-08193 Bellaterra, Catalonia, Spain
Puig, P:
Univ Autonoma Barcelona, Dept Matemat, Bellaterra, Spain
Ctr Recerca Matemat, Bellaterra, Spain
Ribas, M:
Hosp Santa Creu & Sant Pau, Serv Radiofis & Radioproteccio, Barcelona, Spain
Carrasco, P:
Hosp Santa Creu & Sant Pau, Serv Radiofis & Radioproteccio, Barcelona, Spain
Armengol, G:
Univ Autonoma Barcelona, Dept Biol Anim Biol Vegetal & Ecol, Unitat Antropol Biol, E-08193 Bellaterra, Catalonia, Spain
Barquinero, JF:
Univ Autonoma Barcelona, Dept Biol Anim Biol Vegetal & Ecol, Unitat Antropol Biol, E-08193 Bellaterra, Catalonia, Spain
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