Eye bank and theatre factors for positive microbiological culture of corneoscleral rim and cornea storage medium in the real-world
Por:
Sabater-Cruz, N, Otero, N, Dotti-Boada, M, Rios, J, Gris, O, Guell, JL, Vilarrodona, A, Casaroli-Marano, RP
Publicada:
1 nov 2021
Ahead of Print:
1 ene 2021
Resumen:
Objectives To evaluate microbiological culture rate and positivity of corneoscleral rim and cornea storage media as well as possible risk factors for contamination with real-world data.
Methods Data of consecutive cornea donors implanted in the reference centre from January 2013 to January 2018 were reviewed. Information about cornea characteristics (donor demographic data, endothelial cell density, type of cornea conservation, days of storage, and precut vs full-thickness tissue), and microbiological culture information (corneoscleral rim vs storage sample, positive result) were statistically analysed.
Results During the study period, 1369 corneas (737 donors) were implanted. Cultures were performed in 76.8% (n = 1052) of them and were positive in 3.2% of cases, mainly bacteria (84.4%). Corneas preserved in hypothermia represented 61.8% of all positive microbiology results (p < 0.001). Other analysed risk factors did not reach statistically significant association with microbiological positivity. None of the 34 cases with positive microbiological cultures reported ocular infection for the recipients in at least 6 months' follow-up.
Conclusions Microbiological tests rate in real-world practice are high despite not being compulsory. Organotypic cultured corneas showed a statistically less positivity in corneoscleral and storage medium than hypothermic ones, resulting in another advantage of this kind of cornea storage. Although precut corneas are thought to present less microbiological positivity, a statistically significant association was not found in the present study.
Filiaciones:
Sabater-Cruz, N:
Hosp Clin Barcelona, Barcelona, Spain
Univ Barcelona, Sch Med, Dept Surg, Barcelona, Spain
Otero, N:
Barcelona Tissue Bank BST BTB, Barcelona, Spain
Dotti-Boada, M:
Hosp Clin Barcelona, Barcelona, Spain
Rios, J:
Hosp Clin Barcelona, Barcelona, Spain
Univ Autonoma Barcelona, Sch Med, Med Stat Core Facil IDIBAPS, Barcelona, Spain
Univ Autonoma Barcelona, Sch Med, Biostat Unit, Barcelona, Spain
Gris, O:
Inst Microcirugia Ocular IMO, Barcelona, Spain
Guell, JL:
Inst Microcirugia Ocular IMO, Barcelona, Spain
Vilarrodona, A:
Barcelona Tissue Bank BST BTB, Barcelona, Spain
Casaroli-Marano, RP:
Hosp Clin Barcelona, Barcelona, Spain
Univ Barcelona, Sch Med, Dept Surg, Barcelona, Spain
Barcelona Tissue Bank BST BTB, Barcelona, Spain
Open Access
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