Priming human adipose-derived mesenchymal stem cells for corneal surface regeneration
Por:
Nieto-Nicolau, N, Martinez-Conesa, EM, Fuentes-Julian, S, Arnalich-Montiel, F, Garcia-Tunon, I, De Miguel, MP, Casaroli-Marano, RP
Publicada:
1 jun 2021
Ahead of Print:
1 may 2021
Resumen:
Limbal stem cells (LSC) maintain the transparency of the corneal epithelium. Chemical burns lead the loss of LSC inducing an up-regulation of pro-inflammatory and pro-angiogenic factors, triggering corneal neovascularization and blindness. Adipose tissue-derived mesenchymal stem cells (AT-MSC) have shown promise in animal models to treat LSC deficiency (LSCD), but there are not studies showing their efficacy when primed with different media before transplantation. We cultured AT-MSC with standard medium and media used to culture LSC for clinical application. We demonstrated that different media changed the AT-MSC paracrine secretion showing different paracrine effector functions in an in vivo model of chemical burn and in response to a novel in vitro model of corneal inflammation by alkali induction. Treatment of LSCD with AT-MSC changed the angiogenic and inflammatory cytokine profile of mice corneas. AT-MSC cultured with the medium that improved their cytokine secretion, enhanced the anti-angiogenic and anti-inflammatory profile of the treated corneas. Those corneas also presented better outcome in terms of corneal transparency, neovascularization and histologic reconstruction. Priming human AT-MSC with LSC specific medium can potentiate their ability to improve corneal wound healing, decrease neovascularization and inflammation modulating paracrine effector functions in an in vivo optimized rat model of LSCD.
Filiaciones:
Nieto-Nicolau, N:
Univ Barcelona, Dept Cell Biol, CellTec UB, Barcelona, Spain
Banc Sang Teixits BST, Barcelona Tissue Bank BTB, Barcelona, Spain
Inst Biomed Res IIB St Pau SGR1113, Barcelona, Spain
Martinez-Conesa, EM:
Banc Sang Teixits BST, Barcelona Tissue Bank BTB, Barcelona, Spain
Inst Biomed Res IIB St Pau SGR1113, Barcelona, Spain
Fuentes-Julian, S:
La Paz Hosp Res Inst IdiPAZ, Cell Engn Lab, Madrid, Spain
Arnalich-Montiel, F:
La Paz Hosp Res Inst IdiPAZ, Cell Engn Lab, Madrid, Spain
Garcia-Tunon, I:
La Paz Hosp Res Inst IdiPAZ, Cell Engn Lab, Madrid, Spain
De Miguel, MP:
La Paz Hosp Res Inst IdiPAZ, Cell Engn Lab, Madrid, Spain
Casaroli-Marano, RP:
Univ Barcelona, Dept Cell Biol, CellTec UB, Barcelona, Spain
Banc Sang Teixits BST, Barcelona Tissue Bank BTB, Barcelona, Spain
Inst Biomed Res IIB St Pau SGR1113, Barcelona, Spain
Univ Barcelona, Sch Med, Dept Surg, Barcelona, Spain
Univ Barcelona, Hosp Clin Barcelona, Barcelona, Spain
gold, Green Published
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