Nicotinamide Protects Against Diet-Induced Body Weight Gain, Increases Energy Expenditure, and Induces White Adipose Tissue Beiging
Por:
Mendez-Lara, KA, Rodriguez-Millan, E, Sebastian, D, Blanco-Soto, R, Camacho, M, Nan, MN, Diarte-Anazco, EMG, Mato, E, Lope-Piedrafita, S, Roglans, N, Laguna, JC, Alonso, N, Mauricio, D, Zorzano, A, Villarroya, F, Villena, JA, Blanco-Vaca, F, Julve, J
Publicada:
1 jun 2021
Ahead of Print:
1 may 2021
Resumen:
Scope Interventions that boost NAD(+) availability are of potential therapeutic interest for obesity treatment. The potential of nicotinamide (NAM), the amide form of vitamin B3 and a physiological precursor of nicotinamide adenine dinucleotide (NAD)(+), in preventing weight gain has not previously been studied in vivo. Other NAD(+) precursors have been shown to decrease weight gain; however, their impact on adipose tissue is not addressed.
Methods and results Two doses of NAM (high dose: 1% and low dose: 0.25%) are given by drinking water to C57BL/6J male mice, starting at the same time as the high-fat diet feeding. NAM supplementation protects against diet-induced obesity by augmenting global body energy expenditure in C57BL/6J male mice. The manipulation markedly alters adipose morphology and metabolism, particularly in inguinal (i) white adipose tissue (iWAT). An increased number of brown and beige adipocyte clusters, protein abundance of uncoupling protein 1 (UCP1), mitochondrial activity, adipose NAD(+), and phosphorylated AMP-activated protein kinase (P-AMPK) levels are observed in the iWAT of treated mice. Notably, a significant improvement in hepatic steatosis, inflammation, and glucose tolerance is also observed in NAM high-dose treated mice.
Conclusion NAM influences whole-body energy expenditure by driving changes in the adipose phenotype. Thus, NAM is an attractive potential treatment for preventing obesity and associated complications.
Filiaciones:
Mendez-Lara, KA:
Hosp Santa Creu & Sant Pau, Inst Recerca, Barcelona 08041, Spain
Hosp Santa Creu & Sant Pau, IIB St Pau Barcelona, Inst Invest Biomed, Barcelona 08041, Spain
Univ Autonoma Barcelona UAB, Dept Bioquim & Biol Mol, Barcelona 08193, Spain
Rodriguez-Millan, E:
Hosp Santa Creu & Sant Pau, Inst Recerca, Barcelona 08041, Spain
Hosp Santa Creu & Sant Pau, IIB St Pau Barcelona, Inst Invest Biomed, Barcelona 08041, Spain
Sebastian, D:
Univ Barcelona UB, Dept Bioquim & Biomed, Barcelona 08028, Spain
Barcelona Inst Sci & Technol, Inst Res Biomed IRB Barcelona, Barcelona 08028, Spain
CIBERDEM, Ctr Invest Biomed Red Diabet & Enfermedades Metab, Madrid 28028, Spain
Blanco-Soto, R:
CIBER BBN, Ctr Invest Biomed Red Bioingn Biomat & Nanomed, Madrid 28028, Spain
Camacho, M:
Hosp Santa Creu & Sant Pau, Inst Recerca, Barcelona 08041, Spain
Hosp Santa Creu & Sant Pau, IIB St Pau Barcelona, Inst Invest Biomed, Barcelona 08041, Spain
Nan, MN:
Hosp Santa Creu & Sant Pau, IIB St Pau, Serv Bioquim, Barcelona 08041, Spain
Diarte-Anazco, EMG:
Hosp Santa Creu & Sant Pau, Inst Recerca, Barcelona 08041, Spain
Hosp Santa Creu & Sant Pau, IIB St Pau Barcelona, Inst Invest Biomed, Barcelona 08041, Spain
Mato, E:
CIBER BBN, Ctr Invest Biomed Red Bioingn Biomat & Nanomed, Madrid 28028, Spain
Lope-Piedrafita, S:
CIBER BBN, Ctr Invest Biomed Red Bioingn Biomat & Nanomed, Madrid 28028, Spain
Univ Autonoma Barcelona, Serv Ressonancia Magnet Nucl, Cerdanyola Valles Barcel 08193, Spain
Roglans, N:
Univ Barcelona, Fac Farm, Dept Farmacol Toxicol & Quim Terapeut, Barcelona 08028, Spain
Laguna, JC:
Univ Barcelona, Fac Farm, Dept Farmacol Toxicol & Quim Terapeut, Barcelona 08028, Spain
Alonso, N:
CIBERDEM, Ctr Invest Biomed Red Diabet & Enfermedades Metab, Madrid 28028, Spain
Hosp Badalona Germans Trias & Pujol, Servei Endocrinol, Barcelona 08916, Spain
Mauricio, D:
Univ Barcelona UB, Dept Bioquim & Biomed, Barcelona 08028, Spain
CIBERDEM, Ctr Invest Biomed Red Diabet & Enfermedades Metab, Madrid 28028, Spain
Hosp Santa Creu & Sant Pau, IIB St Pau Barcelona, Serv Endocrinol & Nutr, Barcelona 08041, Spain
Zorzano, A:
Univ Barcelona UB, Dept Bioquim & Biomed, Barcelona 08028, Spain
Barcelona Inst Sci & Technol, Inst Res Biomed IRB Barcelona, Barcelona 08028, Spain
CIBERDEM, Ctr Invest Biomed Red Diabet & Enfermedades Metab, Madrid 28028, Spain
Villarroya, F:
Univ Barcelona UB, Dept Bioquim & Biomed, Barcelona 08028, Spain
CIBERDEM, Ctr Invest Biomed Red Diabet & Enfermedades Metab, Madrid 28028, Spain
CIBEROBN, Ctr Invest Biomed Red Fisiopatol Obesidad & Nutr, Madrid 28028, Spain
Villena, JA:
Inst Recerca Vall dHebron, Unitat Diabetis & Metab, Lab Metab & Obesitat, Barcelona 08035, Spain
Blanco-Vaca, F:
Univ Autonoma Barcelona UAB, Dept Bioquim & Biol Mol, Barcelona 08193, Spain
CIBERDEM, Ctr Invest Biomed Red Diabet & Enfermedades Metab, Madrid 28028, Spain
Hosp Santa Creu & Sant Pau, IIB St Pau, Serv Bioquim, Barcelona 08041, Spain
Julve, J:
Hosp Santa Creu & Sant Pau, Inst Recerca, Barcelona 08041, Spain
Hosp Santa Creu & Sant Pau, IIB St Pau Barcelona, Inst Invest Biomed, Barcelona 08041, Spain
Univ Autonoma Barcelona UAB, Dept Bioquim & Biol Mol, Barcelona 08193, Spain
CIBERDEM, Ctr Invest Biomed Red Diabet & Enfermedades Metab, Madrid 28028, Spain
Open Access
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