Isolation of human fibroadipogenic progenitors and satellite cells from frozen muscle biopsies
Por:
Suarez-Calvet, X, Fernandez-Simon, E, Pinol-Jurado, P, Alonso-Perez, J, Carrasco-Rozas, A, Lleixa, C, Lopez-Fernandez, S, Pons, G, Soria, L, Bigot, A, Mouly, V, Illa, I, Gallardo, E, Jaiswal, JK, Diaz-Manera, J
Publicada:
1 sep 2021
Resumen:
Skeletal muscle contains multiple cell types that work together to maintain tissue homeostasis. Among these, satellite cells (SC) and fibroadipogenic progenitors cells (FAPs) are the two main stem cell pools. Studies of these cells using animal models have shown the importance of interactions between these cells in repair of healthy muscle, and degeneration of dystrophic muscle. Due to the unavailability of fresh patient muscle biopsies, similar analysis of interactions between human FAPs and SCs is limited especially among the muscular dystrophy patients. To address this issue here we describe a method that allows the use of frozen human skeletal muscle biopsies to simultaneously isolate and grow SCs and FAPs from healthy or dystrophic patients. We show that while the purified SCs differentiate into mature myotubes, purified FAPs can differentiate into adipocytes or fibroblasts demonstrating their multipotency. We find that these FAPs can be immortalized and the immortalized FAPs (iFAPs) retain their multipotency. These approaches open the door for carrying out personalized analysis of patient FAPs and interactions with the SCs that lead to muscle loss.
Filiaciones:
Suarez-Calvet, X:
Hosp Santa Creu & Sant Pau, Neuromuscular Dis Unit, Neurol Dept, C St Quinti 77, Barcelona 08041, Spain
Biomed Res Inst St Pau IIB St Pau, C St Quinti 77, Barcelona 08041, Spain
Ctr Invest Biomed Red Enfermedades Raras CIBERER, Madrid, Spain
Fernandez-Simon, E:
Hosp Santa Creu & Sant Pau, Neuromuscular Dis Unit, Neurol Dept, C St Quinti 77, Barcelona 08041, Spain
Biomed Res Inst St Pau IIB St Pau, C St Quinti 77, Barcelona 08041, Spain
Univ Newcastle, John Walton Muscular Dystrophy Res Ctr, Newcastle Upon Tyne, Tyne & Wear, England
Pinol-Jurado, P:
Hosp Santa Creu & Sant Pau, Neuromuscular Dis Unit, Neurol Dept, C St Quinti 77, Barcelona 08041, Spain
Biomed Res Inst St Pau IIB St Pau, C St Quinti 77, Barcelona 08041, Spain
Univ Newcastle, John Walton Muscular Dystrophy Res Ctr, Newcastle Upon Tyne, Tyne & Wear, England
Alonso-Perez, J:
Hosp Santa Creu & Sant Pau, Neuromuscular Dis Unit, Neurol Dept, C St Quinti 77, Barcelona 08041, Spain
Biomed Res Inst St Pau IIB St Pau, C St Quinti 77, Barcelona 08041, Spain
Carrasco-Rozas, A:
Hosp Santa Creu & Sant Pau, Neuromuscular Dis Unit, Neurol Dept, C St Quinti 77, Barcelona 08041, Spain
Biomed Res Inst St Pau IIB St Pau, C St Quinti 77, Barcelona 08041, Spain
Lleixa, C:
Hosp Santa Creu & Sant Pau, Neuromuscular Dis Unit, Neurol Dept, C St Quinti 77, Barcelona 08041, Spain
Biomed Res Inst St Pau IIB St Pau, C St Quinti 77, Barcelona 08041, Spain
Lopez-Fernandez, S:
Autonomous Univ Barcelona, Plast Surg Dept, Hosp Santa Creu & St Pau, Barcelona, Spain
Pons, G:
Autonomous Univ Barcelona, Plast Surg Dept, Hosp Santa Creu & St Pau, Barcelona, Spain
Soria, L:
Autonomous Univ Barcelona, Orthopaed & Traumatol Dept, Hosp Santa Creu & St Pau, Barcelona, Spain
Bigot, A:
Sorbonne Univ, Ctr Res Myol, Inst Myol, Inserm,U974, Paris, France
Mouly, V:
Sorbonne Univ, Ctr Res Myol, Inst Myol, Inserm,U974, Paris, France
Illa, I:
Hosp Santa Creu & Sant Pau, Neuromuscular Dis Unit, Neurol Dept, C St Quinti 77, Barcelona 08041, Spain
Biomed Res Inst St Pau IIB St Pau, C St Quinti 77, Barcelona 08041, Spain
Ctr Invest Biomed Red Enfermedades Raras CIBERER, Madrid, Spain
Gallardo, E:
Hosp Santa Creu & Sant Pau, Neuromuscular Dis Unit, Neurol Dept, C St Quinti 77, Barcelona 08041, Spain
Biomed Res Inst St Pau IIB St Pau, C St Quinti 77, Barcelona 08041, Spain
Ctr Invest Biomed Red Enfermedades Raras CIBERER, Madrid, Spain
Jaiswal, JK:
George Washington Univ, Sch Med & Hlth Sci, Dept Genom & Precis Med, Washington, DC 20052 USA
Childrens Natl Hosp, Ctr Genet Med Res, Washington, DC USA
Diaz-Manera, J:
Hosp Santa Creu & Sant Pau, Neuromuscular Dis Unit, Neurol Dept, C St Quinti 77, Barcelona 08041, Spain
Biomed Res Inst St Pau IIB St Pau, C St Quinti 77, Barcelona 08041, Spain
Ctr Invest Biomed Red Enfermedades Raras CIBERER, Madrid, Spain
Univ Newcastle, John Walton Muscular Dystrophy Res Ctr, Newcastle Upon Tyne, Tyne & Wear, England
Green Published, hybrid
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