Biofabrication of functional protein nanoparticles through simple His-tag engineering
Por:
Lopez-Laguna, H, Sanchez, JM, Carratala, JV, Rojas-Pena, M, Sanchez-Garcia, L, Parlade, E, Sanchez-Chardi, A, Volta-Duran, E, Serna, N, Cano-Garrido, O, Flores, S, Ferrer-Miralles, N, Nolan, V, de Marco, A, Roher, N, Unzueta, U, Vazquez, E, Villaverde, A
Publicada:
13 sep 2021
Ahead of Print:
1 ago 2021
Resumen:
We have developed a simple, robust, and fully transversal approach for the a-la-carte fabrication of functional multimeric nanoparticles with potential biomedical applications, validated here by a set of diverse and unrelated polypeptides. The proposed concept is based on the controlled coordination between Zn2+ ions and His residues in His-tagged proteins. This approach results in a spontaneous and reproducible protein assembly as nanoscale oligomers that keep the original functionalities of the protein building blocks. The assembly of these materials is not linked to particular polypeptide features, and it is based on an environmentally friendly and sustainable approach. The resulting nanoparticles, with dimensions ranging between 10 and 15 nm, are regular in size, are architecturally stable, are fully functional, and serve as intermediates in a more complex assembly process, resulting in the formation of microscale protein materials. Since most of the recombinant proteins produced by biochemical and biotechnological industries and intended for biomedical research are His-tagged, the green biofabrication procedure proposed here can be straightforwardly applied to a huge spectrum of protein species for their conversion into their respective nanostructured formats.
Filiaciones:
Lopez-Laguna, H:
Univ Autonoma Barcelona, Inst Biotecnol & Biomed, Barcelona 08193, Spain
Univ Autonoma Barcelona, Dept Genet & Microbiol, Barcelona 08193, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, Madrid 28029, Spain
Sanchez, JM:
Univ Autonoma Barcelona, Inst Biotecnol & Biomed, Barcelona 08193, Spain
Univ Autonoma Barcelona, Dept Genet & Microbiol, Barcelona 08193, Spain
Univ Nacl Cordoba, Fac Ciencias Exactas Fis & Nat, ICTA, RA-5016 Cordoba, Argentina
Univ Nacl Cordoba, Dept Quim, RA-5016 Cordoba, Argentina
Univ Nacl Cordoba, CONICET, Inst Invest Biol & Tecnoldg IIByT, RA-5016 Cordoba, Argentina
Carratala, JV:
Univ Autonoma Barcelona, Inst Biotecnol & Biomed, Barcelona 08193, Spain
Univ Autonoma Barcelona, Dept Genet & Microbiol, Barcelona 08193, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, Madrid 28029, Spain
Rojas-Pena, M:
Univ Autonoma Barcelona, Inst Biotecnol & Biomed, Barcelona 08193, Spain
Sanchez-Garcia, L:
Univ Autonoma Barcelona, Inst Biotecnol & Biomed, Barcelona 08193, Spain
Univ Autonoma Barcelona, Dept Genet & Microbiol, Barcelona 08193, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, Madrid 28029, Spain
Parlade, E:
Univ Autonoma Barcelona, Inst Biotecnol & Biomed, Barcelona 08193, Spain
Univ Autonoma Barcelona, Dept Genet & Microbiol, Barcelona 08193, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, Madrid 28029, Spain
Sanchez-Chardi, A:
Univ Autonoma Barcelona, Serv Microscopia, Barcelona 08193, Spain
Univ Barcelona, Fac Biol, Dept Biol Evolut Ecol & Ciencies Ambientals, Barcelona 08028, Spain
Volta-Duran, E:
Univ Autonoma Barcelona, Inst Biotecnol & Biomed, Barcelona 08193, Spain
Univ Autonoma Barcelona, Dept Genet & Microbiol, Barcelona 08193, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, Madrid 28029, Spain
Serna, N:
Univ Autonoma Barcelona, Inst Biotecnol & Biomed, Barcelona 08193, Spain
Univ Autonoma Barcelona, Dept Genet & Microbiol, Barcelona 08193, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, Madrid 28029, Spain
Univ Autonoma Barcelona, Nanoligent SL, Edifici Eureka, Barcelona 08193, Spain
Cano-Garrido, O:
Univ Autonoma Barcelona, Inst Biotecnol & Biomed, Barcelona 08193, Spain
Univ Autonoma Barcelona, Dept Genet & Microbiol, Barcelona 08193, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, Madrid 28029, Spain
Univ Autonoma Barcelona, Nanoligent SL, OCG, Edifici Eureka, Barcelona 08193, Spain
Flores, S:
Univ Nacl Cordoba, Fac Ciencias Exactas Fis & Nat, ICTA, RA-5016 Cordoba, Argentina
Univ Nacl Cordoba, Dept Quim, RA-5016 Cordoba, Argentina
Univ Nacl Cordoba, CONICET, Inst Invest Biol & Tecnoldg IIByT, RA-5016 Cordoba, Argentina
Ferrer-Miralles, N:
Univ Autonoma Barcelona, Inst Biotecnol & Biomed, Barcelona 08193, Spain
Univ Autonoma Barcelona, Dept Genet & Microbiol, Barcelona 08193, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, Madrid 28029, Spain
Nolan, V:
Univ Nacl Cordoba, Fac Ciencias Exactas Fis & Nat, ICTA, RA-5016 Cordoba, Argentina
Univ Nacl Cordoba, Dept Quim, RA-5016 Cordoba, Argentina
Univ Nacl Cordoba, CONICET, Inst Invest Biol & Tecnoldg IIByT, RA-5016 Cordoba, Argentina
de Marco, A:
Univ Nova Gorica, Lab Environm & Life Sci, Nova Gorica 5000, Slovenia
Roher, N:
Univ Autonoma Barcelona, Inst Biotecnol & Biomed, Barcelona 08193, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, Madrid 28029, Spain
Univ Autonoma Barcelona, Dept Biol Cellular Fisiol Anim & Immunol, Barcelona 08193, Spain
Unzueta, U:
Univ Autonoma Barcelona, Dept Genet & Microbiol, Barcelona 08193, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, Madrid 28029, Spain
Biomed Res Inst St Pau IIB St Pau, Barcelona 08025, Spain
Vazquez, E:
Univ Autonoma Barcelona, Inst Biotecnol & Biomed, Barcelona 08193, Spain
Univ Autonoma Barcelona, Dept Genet & Microbiol, Barcelona 08193, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, Madrid 28029, Spain
Villaverde, A:
Univ Autonoma Barcelona, Inst Biotecnol & Biomed, Barcelona 08193, Spain
Univ Autonoma Barcelona, Dept Genet & Microbiol, Barcelona 08193, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, Madrid 28029, Spain
Green Published, hybrid
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