Tumour TIF1 mutations and loss of heterozygosity related to cancer-associated myositis
Por:
Pinal-Fernandez, I, Ferrer-Fabregas, B, Trallero-Araguas, E, Balada, E, Martinez, MA, Milisenda, JC, Aparicio-Espanol, G, Labrador-Horrillo, M, Garcia-Patos, V, Grau-Junyent, JM, Selva-O'Callaghan, A
Publicada:
1 feb 2018
Resumen:
Objectives. To analyse the influence of genetic alterations and differential expression of transcription intermediary factor 1 (TIF1) genes in the pathophysiology of cancer-associated myositis (CAM).
Methods. Paired blood and tumour DNA samples from patients with anti-TIF1 gamma-positive CAM and from controls were analysed by whole-exome sequencing for the presence of somatic mutations and loss of heterozygosity (LOH) in their TIF1 genes. The genesis and maintenance of the autoimmune process were investigated immunohistochemically by studying TIF1 gamma expression in the different tissues involved in CAM (skin, muscle and tumour) based on the immunohistochemical H-score.
Results. From seven patients with anti-TIF1 gamma-positive CAM, we detected one somatic mutation and five cases of LOH in one or more of the four TIF1 genes compared with just one case of LOH in tumours from TIF1 gamma-negative myositis patients (86% vs 17%; P = 0.03). Compared with type-matched control tumours from non-myositis patients, TIF1 gamma staining was more intense in tumours from anti-TIF1 gamma-positive patients (H-score 255 vs 196;P = 0.01). Also, TIF1 gamma staining in muscle was slightly more intense in anti-TIF1 gamma-positive than in anti-TIF1 gamma-negative myositis (H-score 22 vs 5; P = 0.03). In contrast, intense TIF1 gamma staining was detected in the skin of both myositis and control patients.
Conclusion. Tumours from paraneoplastic anti-TIF1 gamma-positive patients showed an increased number of genetic alterations, such as mutations and LOH, in TIF1 genes. These genetic alterations, in the context of a high expression of TIF1 gamma in the tumour, muscle and skin of these patients may be key to understanding the genesis of paraneoplastic myositis.
Filiaciones:
Pinal-Fernandez, I:
Vall dHebron Univ Hosp, Internal Med Dept, Barcelona, Spain
Univ Autonoma Barcelona, Med Dept, Barcelona, Spain
NIAMS, NIH, Muscle Dis Unit, Bethesda, MD 20892 USA
Ferrer-Fabregas, B:
Vall dHebron Univ Hosp, Pathol Dept, Barcelona, Spain
Trallero-Araguas, E:
Vall dHebron Univ Hosp, Internal Med Dept, Barcelona, Spain
Balada, E:
Vall dHebron Univ Hosp, Internal Med Dept, Barcelona, Spain
Martinez, MA:
Hosp Santa Creu & Sant Pau, Immunol Dept, Barcelona, Spain
Milisenda, JC:
Univ Barcelona, Muscle Res Grp, Inst Invest Biomed August Pi I Sunyer, Barcelona, Spain
Univ Barcelona, Ciberer, Hosp Clin Prov, Barcelona, Spain
Aparicio-Espanol, G:
Vall dHebron Univ Hosp, Dermatol Dept, Barcelona, Spain
Labrador-Horrillo, M:
Vall dHebron Univ Hosp, Internal Med Dept, Barcelona, Spain
Garcia-Patos, V:
Univ Autonoma Barcelona, Med Dept, Barcelona, Spain
Vall dHebron Univ Hosp, Dermatol Dept, Barcelona, Spain
Grau-Junyent, JM:
Univ Barcelona, Muscle Res Grp, Inst Invest Biomed August Pi I Sunyer, Barcelona, Spain
Univ Barcelona, Ciberer, Hosp Clin Prov, Barcelona, Spain
Selva-O'Callaghan, A:
Vall dHebron Univ Hosp, Internal Med Dept, Barcelona, Spain
Univ Autonoma Barcelona, Med Dept, Barcelona, Spain
Green Published, Bronze
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