Osmotic gradient ektacytometry: A valuable screening test for hereditary spherocytosis and other red blood cell membrane disorders
Por:
Llaudet-Planas, E, Vives-Corrons, JL, Rizzuto, V, Gomez-Ramirez, P, Navarro, JS, Sibina, MTC, Garcia-Bernal, M, Llobet, AR, Badell, I, Velasco-Puyo, P, Dapena, JL, Manu-Pereira, MM
Publicada:
1 feb 2018
Resumen:
IntroductionNew generation osmotic gradient ektacytometry has become a powerful procedure for measuring red blood cell deformability and therefore for the diagnosis of red blood cell membrane disorders. In this study, we aim to provide further support to the usefulness of osmotic gradient ektacytometry for the differential diagnosis of hereditary spherocytosis by measuring the optimal cutoff values of the parameters provided by this technique.
MethodsA total of 65 cases of hereditary spherocytosis, 7 hereditary elliptocytosis, 3 hereditary xerocytosis, and 171 normal controls were analyzed with osmotic gradient ektacytometry in addition to the routine red blood cell laboratory techniques. The most robust osmoscan parameters for hereditary spherocytosis diagnosis were determined using receiver operating characteristic curve analysis.
ResultsThe best diagnostic criteria for hereditary spherocytosis were the combination of decreased minimal elongation index up to 3% and increased minimal osmolality point up to 5.2% when compared to the mean of controls. Using this established criterion, osmotic gradient ektacytometry reported a sensitivity of 93.85% and a specificity of 98.38% for the diagnosis of hereditary spherocytosis.
ConclusionOsmotic gradient ektacytometry is an effective diagnostic test for hereditary spherocytosis and enables its differential diagnosis with other red blood cell membrane diseases based on specific pathology profiles.
Filiaciones:
Llaudet-Planas, E:
Univ Barcelona, Hosp Clin Barcelona, Inst Invest Biomed August Pi & Sunyer IDIBAPS, Red Cell Pathol Unit, Barcelona, Spain
Univ Autonoma Barcelona, Red Cell Pathol Unit, Josep Carreras Leukemia Res Inst IJC, Badalona, Spain
Vives-Corrons, JL:
Univ Barcelona, Hosp Clin Barcelona, Inst Invest Biomed August Pi & Sunyer IDIBAPS, Red Cell Pathol Unit, Barcelona, Spain
Univ Autonoma Barcelona, Red Cell Pathol Unit, Josep Carreras Leukemia Res Inst IJC, Badalona, Spain
Rizzuto, V:
Univ Autonoma Barcelona, Red Cell Pathol Unit, Josep Carreras Leukemia Res Inst IJC, Badalona, Spain
Gomez-Ramirez, P:
Univ Barcelona, Hosp Clin Barcelona, Inst Invest Biomed August Pi & Sunyer IDIBAPS, Red Cell Pathol Unit, Barcelona, Spain
Navarro, JS:
HIUNJ, FIB, CIBERER, Serv Hematol Pediat, Madrid, Spain
Sibina, MTC:
Hosp Gen Granollers, Dept Pediat, Granollers, Spain
Garcia-Bernal, M:
Hosp Univ Mutua Terrassa, Pediat Hematol & Oncol Dept, Terrassa, Spain
Llobet, AR:
Univ Barcelona, Hosp St Joan Deu Barcelona, Pediat Hematol & Oncol Dept, Barcelona, Spain
Badell, I:
Univ Autonoma Barcelona, Unidad Hematol Oncol & Trasplante Hematopoyet, Hosp Univ Santa Creu & St Pau, Barcelona, Spain
Velasco-Puyo, P:
Univ Autonoma Barcelona, Pediat Oncol & Hematol Dept, Hosp Univ Vall dHebron, Barcelona, Spain
Dapena, JL:
Univ Autonoma Barcelona, Pediat Oncol & Hematol Dept, Hosp Univ Vall dHebron, Barcelona, Spain
Manu-Pereira, MM:
Univ Barcelona, Hosp Clin Barcelona, Inst Invest Biomed August Pi & Sunyer IDIBAPS, Red Cell Pathol Unit, Barcelona, Spain
Univ Autonoma Barcelona, Red Cell Pathol Unit, Josep Carreras Leukemia Res Inst IJC, Badalona, Spain
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