First Description of bla(NDM-7) Carried on an IncX4 Plasmid in Escherichia coli ST679 Isolated in Spain
Por:
Espinal, P, Miro, E, Segura, C, Gomez, L, Plasencia, V, Coll, P, Navarro, F
Publicada:
1 mar 2018
Resumen:
This study describes the molecular characterization of an NDM-7 carbapenemase-producing Escherichia coli strain Ec188, recovered from a rectal swab of a male patient who had travelled to Pakistan before his hospitalization at the Hospital del Mar in Barcelona, Spain. The Ec188 isolate, assigned to a new multilocus sequence type ST679, was resistant to all beta-lactams, aminoglycosides (gentamicin, tobramycin, and with reduced susceptibility to amikacin), and ciprofloxacin. The bla(NDM-7) gene was located on a 50kb IncX4 plasmid (pEc188-NDM7), both in the original and transconjugant strains. In addition, bla(CTX-M-15) was located on a 150kb IncFIA plasmid and bla(CMY-2) on a 95kb undetermined plasmid type, only in the wild-type strain. The immediate genetic surroundings of bla(NDM-7) included the bleo, trpf, and dsbC genes, and it was flanked by the insertion sequences IS26 and ISAba125, which appeared interrupted by IS5. The res and parA genes were found in the same orientation downstream of the IS26 element. To our knowledge, this is the first report of an NDM-7-carbapenemase carried on an IncX4 plasmid, as well as the first E. coli strain belonging to ST679 harboring an NDM -lactamase, possibly associated with previous travel to Pakistan. In addition, this study highlights the dissemination of NDM variants accompanied by IncX-type plasmids.
Filiaciones:
Espinal, P:
Hosp Santa Creu & Sant Pau, IIB St Pau, Serv Microbiol, C St Quinti 89, Barcelona 08041, Spain
Miro, E:
Hosp Santa Creu & Sant Pau, IIB St Pau, Serv Microbiol, C St Quinti 89, Barcelona 08041, Spain
Segura, C:
Reference Lab Catalonia, Dept Microbiol, Barcelona, Spain
Gomez, L:
Hosp Santa Creu & Sant Pau, IIB St Pau, Serv Microbiol, C St Quinti 89, Barcelona 08041, Spain
Plasencia, V:
Reference Lab Catalonia, Dept Microbiol, Barcelona, Spain
Coll, P:
Hosp Santa Creu & Sant Pau, IIB St Pau, Serv Microbiol, C St Quinti 89, Barcelona 08041, Spain
Univ Autonoma Barcelona, Dept Genet & Microbiol, Barcelona, Spain
Navarro, F:
Univ Autonoma Barcelona, Dept Genet & Microbiol, Barcelona, Spain
Hosp Santa Creu & Sant Pau, IIB St Pau, Serv Microbiol, C St Quinti 89, Barcelona 08041, Spain
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