Antibiotic resistance genes in phage particles isolated from human faeces and induced from clinical bacterial isolates
Por:
Brown-Jaque, M, Calero-Caceres, W, Espinal, P, Rodriguez-Navarro, J, Miro, E, Gonzalez-Lopez, JJ, Cornejo, T, Hurtado, JC, Navarro, F, Muniesa, M
Publicada:
1 mar 2018
Resumen:
Phage particles have emerged as elements with the potential to mobilise antibiotic resistance genes (ARGs) in different environments, including the intestinal habitat. This study aimed to determine the occurrence of ARGs in phage particles present in faecal matter and induced from strains isolated from faeces. Nine ARGs (bla(TEM), bla(CTX-M-1 group), bla(CTX-M-9 group), bla(OXA-48), qnrA, qnrS, mecA, sul1 and armA) were quantified by qPCR in the phage DNA fractions of 150 faecal samples obtained from healthy individuals who had not received antibiotic treatment or travelled abroad in the 3 months prior to sample collection. On the suspicion that the detected particles originated from bacterial flora, 82 Escherichia coli and Klebsiella pneumoniae isolates possessing at least one identified ARG (bla(TEM), bla(CTX-M-1 group), bla(CTX-M-9 group), armA, qnrA, qnrS and sul1) were isolated and their capacity to produce phage particles carrying these ARGs following induction was evaluated. Of 150 samples, 72.7% were positive for at least one ARG, with bla(TEM) and bla(CTX-M-9 group) being the most prevalent and abundant. Of the 82 isolates, 51 (62%) showed an increase in the number of copies of the respective ARG in the phage fraction following induction, with bla(TEM), bla(CTX-M-1 group), bla(CTX-M-9 group) and sul1 being the most abundant. Phages induced from the isolates were further purified and visualised using microscopy and their DNA showed ARG levels of up to 1010 gene copies/mL. This study highlights the abundance of phage particles harbouring ARGs and indicates that bacterial strains in the intestinal habitat could be source of these particles. (C) 2017 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.
Filiaciones:
Brown-Jaque, M:
Univ Barcelona, Dept Genet Microbiol & Stat, Diagonal 643,Floor 0, E-08028 Barcelona, Spain
Calero-Caceres, W:
Univ Barcelona, Dept Genet Microbiol & Stat, Diagonal 643,Floor 0, E-08028 Barcelona, Spain
Espinal, P:
Hosp Santa Creu & Sant Pau, Serv Microbiol, Inst Invest Biomed St Pau, Sant Quinti 89, Barcelona 08041, Spain
Rodriguez-Navarro, J:
Hosp Santa Creu & Sant Pau, Serv Microbiol, Inst Invest Biomed St Pau, Sant Quinti 89, Barcelona 08041, Spain
Univ Autonoma Barcelona, Dept Genet & Microbiol, Barcelona, Spain
Miro, E:
Hosp Santa Creu & Sant Pau, Serv Microbiol, Inst Invest Biomed St Pau, Sant Quinti 89, Barcelona 08041, Spain
Gonzalez-Lopez, JJ:
Hosp Valle De Hebron, Vall dHebron Inst Recerca, Dept Clin Microbiol, Barcelona, Spain
Cornejo, T:
Hosp Valle De Hebron, Vall dHebron Inst Recerca, Dept Clin Microbiol, Barcelona, Spain
Hurtado, JC:
Univ Barcelona, Hosp Clin Barcelona, Barcelona Ctr Int Hlth Res, ISGlobal,Dept Microbiol, Barcelona, Spain
Navarro, F:
Hosp Santa Creu & Sant Pau, Serv Microbiol, Inst Invest Biomed St Pau, Sant Quinti 89, Barcelona 08041, Spain
Univ Autonoma Barcelona, Dept Genet & Microbiol, Barcelona, Spain
Muniesa, M:
Univ Barcelona, Dept Genet Microbiol & Stat, Diagonal 643,Floor 0, E-08028 Barcelona, Spain
Green Accepted
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