Impaired HDL (High-Density Lipoprotein)-Mediated Macrophage Cholesterol Efflux in Patients With Abdominal Aortic Aneurysm-Brief Report
Por:
Martinez-Lopez, D, Cedo, L, Metso, J, Burillo, E, Garcia-Leon, A, Canyelles, M, Lindholt, JS, Torres-Fonseca, M, Blanco-Colio, LM, Vazquez, J, Blanco-Vaca, F, Jauhiainen, M, Martin-Ventura, JL, Escola-Gil, JC
Publicada:
1 nov 2018
Resumen:
Objective The ability of HDL (high-density lipoprotein) to promote macrophage cholesterol efflux is considered the main HDL cardioprotective function. Abdominal aortic aneurysm (AAA) is usually characterized by cholesterol accumulation and macrophage infiltration in the aortic wall. Here, we aim to evaluate the composition of circulating HDL particles and their potential for promoting macrophage cholesterol efflux in AAA subjects.
Approach and Results First, we randomly selected AAA and control subjects from Spain. The AAA patients in the Spanish cohort showed lower plasma apoA-I levels concomitantly associated with low levels of plasma HDL cholesterol and the amount of pre-HDL particles. We determined macrophage cholesterol efflux to apoB-depleted plasma, which contains mature HDL, pre-HDL particles and HDL regulatory proteins. ApoB-depleted plasma from AAA patients displayed an impaired ability to promote macrophage cholesterol efflux. Next, we replicated the experiments with AAA and control subjects derived from Danish cohort. Danish AAA patients also showed lower apoA-I levels and a defective HDL-mediated macrophage cholesterol efflux.
Conclusions AAA patients show impaired HDL-facilitated cholesterol removal from macrophages, which could be mechanistically linked to AAA.
Filiaciones:
Martinez-Lopez, D:
Univ Autonoma, FIIS Fdn Jimenez Diaz, Lab Patol Vasc, Av Reyes Catolicos 2, Madrid 28040, Spain
Univ Autonoma, FIIS Fdn Jimenez Diaz, CIBER Enfermedades Cardiovasc CIBERCV, Av Reyes Catolicos 2, Madrid 28040, Spain
Cedo, L:
Inst Invest Biomed IIB St Pau, Antoni M Claret 167, Barcelona 08025, Spain
CIBER Diabet & Enfermedades Metabol Asociadas CIB, Barcelona, Spain
Metso, J:
Biomedicum, Minerva Fdn, Inst Med Res, Helsinki, Finland
Biomedicum, Natl Inst Hlth & Welf, Genom & Biomarkers Unit, Helsinki, Finland
Burillo, E:
Univ Autonoma, FIIS Fdn Jimenez Diaz, Lab Patol Vasc, Av Reyes Catolicos 2, Madrid 28040, Spain
Univ Autonoma, FIIS Fdn Jimenez Diaz, CIBER Enfermedades Cardiovasc CIBERCV, Av Reyes Catolicos 2, Madrid 28040, Spain
Garcia-Leon, A:
Inst Invest Biomed IIB St Pau, Antoni M Claret 167, Barcelona 08025, Spain
Canyelles, M:
Inst Invest Biomed IIB St Pau, Antoni M Claret 167, Barcelona 08025, Spain
Lindholt, JS:
Odense Univ Hosp, Dept Cardiothorac & Vasc Surg, Elitary Res Ctr Individualized Med Arterial Dis, Odense, Denmark
Torres-Fonseca, M:
Univ Autonoma, FIIS Fdn Jimenez Diaz, Lab Patol Vasc, Av Reyes Catolicos 2, Madrid 28040, Spain
Univ Autonoma, FIIS Fdn Jimenez Diaz, CIBER Enfermedades Cardiovasc CIBERCV, Av Reyes Catolicos 2, Madrid 28040, Spain
Blanco-Colio, LM:
Univ Autonoma, FIIS Fdn Jimenez Diaz, Lab Patol Vasc, Av Reyes Catolicos 2, Madrid 28040, Spain
Univ Autonoma, FIIS Fdn Jimenez Diaz, CIBER Enfermedades Cardiovasc CIBERCV, Av Reyes Catolicos 2, Madrid 28040, Spain
Vazquez, J:
Ctr Nacl Invest Cardiovasc Carlos III CNIC, CIBER Enfermedades Cardiovasc CIBERCV, Cardiovasc Prote Lab, Madrid, Spain
Blanco-Vaca, F:
Inst Invest Biomed IIB St Pau, Antoni M Claret 167, Barcelona 08025, Spain
CIBER Diabet & Enfermedades Metabol Asociadas CIB, Barcelona, Spain
Univ Autonoma Barcelona, Dept Bioquim Biol Mol & Biomed, Barcelona, Spain
Jauhiainen, M:
Biomedicum, Minerva Fdn, Inst Med Res, Helsinki, Finland
Biomedicum, Natl Inst Hlth & Welf, Genom & Biomarkers Unit, Helsinki, Finland
Martin-Ventura, JL:
Univ Autonoma, FIIS Fdn Jimenez Diaz, Lab Patol Vasc, Av Reyes Catolicos 2, Madrid 28040, Spain
Univ Autonoma, FIIS Fdn Jimenez Diaz, CIBER Enfermedades Cardiovasc CIBERCV, Av Reyes Catolicos 2, Madrid 28040, Spain
Escola-Gil, JC:
Inst Invest Biomed IIB St Pau, Antoni M Claret 167, Barcelona 08025, Spain
CIBER Diabet & Enfermedades Metabol Asociadas CIB, Barcelona, Spain
Univ Autonoma Barcelona, Dept Bioquim Biol Mol & Biomed, Barcelona, Spain
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