How to: identify non-tuberculous Mycobacterium species using MALDI-TOF mass spectrometry
Por:
Alcaide, F, Amlerova, J, Bou, G, Ceyssens, PJ, Coll, P, Corcoran, D, Fangous, MS, Gonzalez-Alvarez, I, Gorton, R, Greub, G, Hery-Arnaud, G, Hrabak, J, Ingebretsen, A, Lucey, B, Marekovic, I, Mediavilla-Gradolph, C, Monte, MR, O'Connor, J, O'Mahony, J, Opota, O, O'Reilly, B, Orth-Holler, D, Oviano, M, Palacios, JJ, Palop, B, Pranada, AB, Quiroga, L, Rodriguez-Temporal, D, Ruiz-Serrano, MJ, Tudo, G, Van den Bossche, A, van Ingen, J, Rodriguez-Sanchez, B, European Study Grp Genomics Mol
Publicada:
1 jun 2018
Resumen:
Background: The implementation of MALDI-TOF MS for microorganism identification has changed the routine of the microbiology laboratories as we knew it. Most microorganisms can now be reliably identified within minutes using this inexpensive, user-friendly methodology. However, its application in the identification of mycobacteria isolates has been hampered by the structure of their cell wall. Improvements in the sample processing method and in the available database have proved key factors for the rapid and reliable identification of non-tuberculous mycobacteria isolates using MALDI-TOF MS.
Aims: The main objective is to provide information about the proceedings for the identification of non-tuberculous isolates using MALDI-TOF MS and to review different sample processing methods, available databases, and the interpretation of the results.
Sources: Results from relevant studies on the use of the available MALDI-TOF MS instruments, the implementation of innovative sample processing methods, or the implementation of improved databases are discussed.
Content: Insight about the methodology required for reliable identification of non-tuberculous mycobacteria and its implementation in the microbiology laboratory routine is provided. (C) 2017 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
Filiaciones:
Alcaide, F:
Hosp Univ Bellvitge IDIBELL, Serv Microbiol, Barcelona, Spain
Amlerova, J:
Fac Med Plzen, Biomed Ctr, Plzen, Czech Republic
Bou, G:
Hosp Univ A Coruna, Serv Microbiol, La Coruna, Spain
Ceyssens, PJ:
Sci Inst Publ Hlth, Div Bacterial Dis, Brussels, Belgium
Coll, P:
Hosp St Pau i Santa Creu, Serv Microbiol, Barcelona, Spain
Corcoran, D:
Cork Univ Hosp, Lab Med, Diagnost Directorate, Cork, Ireland
Fangous, MS:
Hop Cavale Blanche, Plateforme Biol, Lab Bacteriol, Brest, France
Gonzalez-Alvarez, I:
Hosp Univ Cent Asturias, Serv Microbiol, Unidad Referencia Reg Micobacterias, Oviedo, Spain
Gorton, R:
Hlth Serv Labs, London, England
Greub, G:
Univ Lausanne, Inst Microbiol, Lausanne, Switzerland
European Study Grp Genom & Mol Diag ESGMD, Basel, Switzerland
Hery-Arnaud, G:
Hop Cavale Blanche, Plateforme Biol, Lab Bacteriol, Brest, France
Hrabak, J:
Fac Med Plzen, Biomed Ctr, Plzen, Czech Republic
Ingebretsen, A:
Oslo Univ Hosp, Dept Microbiol, Oslo, Norway
Lucey, B:
Cork Inst Technol, Dept Biol Sci, Cork, Ireland
Marekovic, I:
Univ Zagreb, Univ Hosp Ctr Zagreb, Sch Med, Dept Clin & Mol Microbiol, Zagreb, Croatia
Mediavilla-Gradolph, C:
Hosp Reg Malaga, Lab Microbiol, Malaga, Spain
Monte, MR:
Univ Barcelona, Hosp Clin Barcelona ISGlobal, Dept Fonaments Clin, Lab Microbiol CDB,Fac Med & Ciencies Salut, Barcelona, Spain
O'Connor, J:
Cork Inst Technol, Dept Biol Sci, Cork, Ireland
O'Mahony, J:
Cork Inst Technol, Dept Biol Sci, Cork, Ireland
Opota, O:
Univ Lausanne, Inst Microbiol, Lausanne, Switzerland
O'Reilly, B:
Cork Univ Hosp, Lab Med, Diagnost Directorate, Cork, Ireland
Orth-Holler, D:
Med Univ Innsbruck, Div Hyg & Med Microbiol, Innsbruck, Austria
Oviano, M:
Hosp Univ A Coruna, Serv Microbiol, La Coruna, Spain
Palacios, JJ:
Hosp Univ Cent Asturias, Serv Microbiol, Unidad Referencia Reg Micobacterias, Oviedo, Spain
Palop, B:
Hosp Reg Malaga, Lab Microbiol, Malaga, Spain
Pranada, AB:
MVZ Dr Eberhard & Partner Dortmund UBAG, Dept Med Microbiol, Dortmund, Germany
Quiroga, L:
Hosp Gen Univ Gregorio Maranon, Inst Invest Sanitaria Gregorio Maranon, Clin Microbiol & Infect Dis Dept, Madrid, Spain
Rodriguez-Temporal, D:
Hosp Univ Bellvitge IDIBELL, Serv Microbiol, Barcelona, Spain
Ruiz-Serrano, MJ:
European Study Grp Genom & Mol Diag ESGMD, Basel, Switzerland
Hosp Gen Univ Gregorio Maranon, Inst Invest Sanitaria Gregorio Maranon, Clin Microbiol & Infect Dis Dept, Madrid, Spain
CIBER Enfermedades Resp CIBERES CB06 06 0058, Madrid, Spain
Tudo, G:
Univ Barcelona, Hosp Clin Barcelona ISGlobal, Dept Fonaments Clin, Lab Microbiol CDB,Fac Med & Ciencies Salut, Barcelona, Spain
Van den Bossche, A:
Sci Inst Publ Hlth, Div Bacterial Dis, Brussels, Belgium
van Ingen, J:
Radboud Univ Nijmegen, Med Ctr, Dept Med Microbiol, Nijmegen, Netherlands
Rodriguez-Sanchez, B:
European Study Grp Genom & Mol Diag ESGMD, Basel, Switzerland
Hosp Gen Univ Gregorio Maranon, Inst Invest Sanitaria Gregorio Maranon, Clin Microbiol & Infect Dis Dept, Madrid, Spain
CIBER Enfermedades Resp CIBERES CB06 06 0058, Madrid, Spain
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