Decapping protein EDC4 regulates DNA repair and phenocopies BRCA1


Por: Hernandez, G, Ramirez, MJ, Minguillon, J, Quiles, P, de Garibay, GR, Aza-Carmona, M, Bogliolo, M, Pujol, R, Prados-Carvajal, R, Fernandez, J, Garcia, N, Lopez, A, Gutierrez-Enriquez, S, Diez, O, Benitez, J, Salinas, M, Teule, A, Brunet, J, Radice, P, Peterlongo, P, Schindler, D, Huertas, P, Puente, XS, Lazaro, C, Pujana, MA, Surralles, J

Publicada: 6 mar 2018
Resumen:
BRCA1 is a tumor suppressor that regulates DNA repair by homologous recombination. Germline mutations in BRCA1 are associated with increased risk of breast and ovarian cancer and BRCA1 deficient tumors are exquisitely sensitive to poly (ADP-ribose) polymerase (PARP) inhibitors. Therefore, uncovering additional components of this DNA repair pathway is of extreme importance for further understanding cancer development and therapeutic vulnerabilities. Here, we identify EDC4, a known component of processing-bodies and regulator of mRNA decapping, as a member of the BRCA1-BRIP1-TOPBP1 complex. EDC4 plays a key role in homologous recombination by stimulating end resection at double-strand breaks. EDC4 deficiency leads to genome instability and hypersensitivity to DNA interstrand cross-linking drugs and PARP inhibitors. Lack-of-function mutations in EDC4 were detected in BRCA1/2-mutation-negative breast cancer cases, suggesting a role in breast cancer susceptibility. Collectively, this study recognizes EDC4 with a dual role in decapping and DNA repair whose inactivation phenocopies BRCA1 deficiency.

Filiaciones:
Hernandez, G:
 Univ Autonoma Barcelona, Dept Genet & Microbiol, Bellaterra 08193, Spain

 Ctr Invest Biomed Red Enfermedades Raras CIBERER, Barcelona 08193, Spain

Ramirez, MJ:
 Univ Autonoma Barcelona, Dept Genet & Microbiol, Bellaterra 08193, Spain

 Ctr Invest Biomed Red Enfermedades Raras CIBERER, Barcelona 08193, Spain

Minguillon, J:
 Univ Autonoma Barcelona, Dept Genet & Microbiol, Bellaterra 08193, Spain

 Ctr Invest Biomed Red Enfermedades Raras CIBERER, Barcelona 08193, Spain

Quiles, P:
 Bellvitge Inst Biomed Res IDIBELL, Hereditary Canc Programme, Catalan Inst Oncol ICO, Barcelona 08908, Spain

de Garibay, GR:
 IDIBELL, ICO, Breast Canc & Syst Biol Lab, Program Canc Therapeut Resistance ProCURE, Barcelona 08908, Spain

Aza-Carmona, M:
 Univ Autonoma Barcelona, Dept Genet & Microbiol, Bellaterra 08193, Spain

 Ctr Invest Biomed Red Enfermedades Raras CIBERER, Barcelona 08193, Spain

Bogliolo, M:
 Univ Autonoma Barcelona, Dept Genet & Microbiol, Bellaterra 08193, Spain

 Ctr Invest Biomed Red Enfermedades Raras CIBERER, Barcelona 08193, Spain

Pujol, R:
 Univ Autonoma Barcelona, Dept Genet & Microbiol, Bellaterra 08193, Spain

 Ctr Invest Biomed Red Enfermedades Raras CIBERER, Barcelona 08193, Spain

Prados-Carvajal, R:
 Univ Seville, Ctr Andaluz Biol Mol & Med Regenerat CABIMER, E-41080 Seville, Spain

 Univ Seville, Dept Genet, E-41080 Seville, Spain

Fernandez, J:
 Bellvitge Inst Biomed Res IDIBELL, Hereditary Canc Programme, Catalan Inst Oncol ICO, Barcelona 08908, Spain

Garcia, N:
 IDIBELL, ICO, Breast Canc & Syst Biol Lab, Program Canc Therapeut Resistance ProCURE, Barcelona 08908, Spain

Lopez, A:
 Girona Biomed Res Inst IDIBGI, ICO, Hereditary Canc Programme, Girona 17007, Spain

Gutierrez-Enriquez, S:
 VHIO, Oncogenet Grp, Barcelona 08035, Spain

Diez, O:
 VHIO, Oncogenet Grp, Barcelona 08035, Spain

 Hosp Univ Vall dHebron, Area Clin & Mol Genet, Barcelona 08035, Spain

Benitez, J:
 Ctr Invest Biomed Red Enfermedades Raras CIBERER, Barcelona 08193, Spain

 Spanish Natl Canc Res Ctr CNIO, Human Canc Genet Program, Madrid 28029, Spain

Salinas, M:
 Bellvitge Inst Biomed Res IDIBELL, Hereditary Canc Programme, Catalan Inst Oncol ICO, Barcelona 08908, Spain

Teule, A:
 Bellvitge Inst Biomed Res IDIBELL, Hereditary Canc Programme, Catalan Inst Oncol ICO, Barcelona 08908, Spain

Brunet, J:
 Bellvitge Inst Biomed Res IDIBELL, Hereditary Canc Programme, Catalan Inst Oncol ICO, Barcelona 08908, Spain

 Girona Biomed Res Inst IDIBGI, ICO, Hereditary Canc Programme, Girona 17007, Spain

Radice, P:
 Fdn IRCCS Ist Ricovero & Cura Carattere Sci, Dept Prevent & Predict Med, Unit Mol Bases Genet Risk & Genet Testing, INT, I-20133 Milan, Italy

Peterlongo, P:
 Fdn IRCCS Ist Nazl Tumori, Dept Prevent & Predict Med, IFOM, Fdn Ist FIRC Oncol Mol, I-20139 Milan, Italy

 Unit Mol Bases Genet Risk & Genet Testing, I-20139 Milan, Italy

Schindler, D:
 Wurzburg Univ, Dept Human Genet, D-97070 Wurzburg, Germany

Puente, XS:
 Univ Oviedo, Dept Biochem & Mol Biol, Inst Univ Oncol, E-33006 Oviedo, Spain

Lazaro, C:
 Bellvitge Inst Biomed Res IDIBELL, Hereditary Canc Programme, Catalan Inst Oncol ICO, Barcelona 08908, Spain

Pujana, MA:
 IDIBELL, ICO, Breast Canc & Syst Biol Lab, Program Canc Therapeut Resistance ProCURE, Barcelona 08908, Spain

 Ctr Invest Biomed Red Oncol CIBERONC, Oviedo 33006, Spain

Surralles, J:
 Univ Autonoma Barcelona, Dept Genet & Microbiol, Bellaterra 08193, Spain

 Ctr Invest Biomed Red Enfermedades Raras CIBERER, Barcelona 08193, Spain

 Hosp Santa Creu & Sant Pau, Dept Genet, Barcelona 08028, Spain

 Hosp Santa Creu & Sant Pau, Biomed Res Inst St Pau IIB St Pau, Barcelona 08028, Spain
ISSN: 20411723





Nature Communications
Editorial
NATURE PUBLISHING GROUP, MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND, Reino Unido
Tipo de documento: Article
Volumen: 9 Número:
Páginas:
WOS Id: 000426659600002
ID de PubMed: 29511213
imagen Gold, Green Published

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