Impact of CSF storage volume on the analysis of Alzheimer's disease biomarkers on an automated platform
Por:
Delaby, C, Munoz, L, Torres, S, Nadal, A, Le Bastard, N, Lehmann, S, Lleo, A, Alcolea, D
Publicada:
1 mar 2019
Resumen:
Objectives: To assess the potential influence of the ratio between the storage tube surface area and the volume of cerebrospinal fluid (CSF) (surface/volume) on the quantifications of four Alzheimer's disease (AD) biomarkers on the Lumipulse G600II automated platform.
Methods: CSF samples of 10 consecutive patients were stored in 2 ml polypropylene tubes containing four different CSF volumes: 1.5 ml, 1 ml, 0.5 ml and 0.25 ml. Concentration of CSF A beta 1-42, A beta 1-40, t-Tau and p-Tau were measured in all aliquots using the LUMIPULSE G600II automated platform from Fujirebio.
Results: Levels of CSF A beta 1-42 and A beta 1-40 were lower in samples stored with lower volumes (higher surface/volume ratios). This decrease was partly compensated by using the ratio A beta 1-42/A beta 1-40. Quantification of t-Tau and p-Tau were not influenced by this pre-analytical condition.
Conclusion: The surface/volume ratio can potentially influence the results of amyloid AD biomarkers. It appears essential to take into account the surface/volume ratio of the storage tubes when quantifying CSF biomarkers in clinical routine.
Filiaciones:
Delaby, C:
Univ Montpellier, CHU Montpellier, Lab Biochim Prote Clin, INSERM U1183, Montpellier, France
Univ Autonoma Barcelona, St Pau Memory Unit, Dept Neurol, Inst Invest Biomed St Pau,Hosp St Pau, Barcelona, Spain
Munoz, L:
Univ Autonoma Barcelona, St Pau Memory Unit, Dept Neurol, Inst Invest Biomed St Pau,Hosp St Pau, Barcelona, Spain
CIBERNED, Ctr Invest Biomed Red Enfermedades Neurodegenerat, Madrid, Spain
Torres, S:
Univ Autonoma Barcelona, St Pau Memory Unit, Dept Neurol, Inst Invest Biomed St Pau,Hosp St Pau, Barcelona, Spain
CIBERNED, Ctr Invest Biomed Red Enfermedades Neurodegenerat, Madrid, Spain
Nadal, A:
SLU, Fujirebio Iberia, Barcelona, Spain
Le Bastard, N:
Fujirebio Europe NV, Ghent, Belgium
Lehmann, S:
Univ Montpellier, CHU Montpellier, Lab Biochim Prote Clin, INSERM U1183, Montpellier, France
Lleo, A:
Univ Autonoma Barcelona, St Pau Memory Unit, Dept Neurol, Inst Invest Biomed St Pau,Hosp St Pau, Barcelona, Spain
CIBERNED, Ctr Invest Biomed Red Enfermedades Neurodegenerat, Madrid, Spain
Alcolea, D:
Univ Autonoma Barcelona, St Pau Memory Unit, Dept Neurol, Inst Invest Biomed St Pau,Hosp St Pau, Barcelona, Spain
CIBERNED, Ctr Invest Biomed Red Enfermedades Neurodegenerat, Madrid, Spain
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