Genomics of Klebsiella pneumoniae ST16 producing NDM-1, CTX-M-15, and OXA-232
Por:
Espinal, P, Nucleo, E, Caltagirone, M, Marchetti, VM, Fernandes, MR, Biscaro, V, Rigoli, R, Carattoli, A, Migliavacca, R, Villa, L
Publicada:
1 mar 2019
Resumen:
Objectives: Genomic characterization of the internationally spread sequence type (ST) 16 carbapenemresistant Klebsiella pneumoniae.
Methods: The complete genomes of three carbapenem producing ST16 K. pneumoniae from Italian patients were analysed by single-nucleotide polymorphism-based phylogeny, core genome multilocus sequence typing, resistance, plasmid, and virulence content and compared with ten genomes of ST16 strains isolated in other countries. Plasmids carrying blaNDM-1 or blaOXA-232 carbapenemase genes were assembled and sequences were analysed.
Results: The internationally spread ST16 K. pneumoniae clone showed variability in terms of distribution of NDM-1 and OXA-232 type carbapenemases. In some ST16 strains, up to six plasmids can be simultaneously present in the same cell, including ColE-like plasmids carrying blaOXA-232 and IncF plasmids carrying blaNDM-1. The differences observed in plasmid, resistance, and virulence content and core genome suggested that there is not a unique, highly conserved ST16 clone, but instead different variants of this lineage circulate worldwide.
Conclusions: The ST16 K. pneumoniae clone has spread worldwide and may become a high-risk clone. (c) 2018 Published by Elsevier Ltd on behalf of European Society of Clinical Microbiology and Infectious Diseases.
Filiaciones:
Espinal, P:
Ist Super Sanita, Dept Infect Dis, Viale Regina Margherita 299, I-00161 Rome, Italy
Inst Invest Biomed St Pau IIB St Pau, Servei Microbiol Hosp Santa Creu & St Pau, Barcelona, Spain
Nucleo, E:
Univ Pavia, Unit Microbiol & Clin Microbiol, Clin Surg Diagnost & Paediat Sci Dept, Pavia, Italy
Caltagirone, M:
Univ Pavia, Unit Microbiol & Clin Microbiol, Clin Surg Diagnost & Paediat Sci Dept, Pavia, Italy
Marchetti, VM:
Univ Pavia, Unit Microbiol & Clin Microbiol, Clin Surg Diagnost & Paediat Sci Dept, Pavia, Italy
Fernandes, MR:
Ist Super Sanita, Dept Infect Dis, Viale Regina Margherita 299, I-00161 Rome, Italy
Univ Sao Paulo, Fac Pharmaceut Sci, Sao Paulo, Brazil
Biscaro, V:
Treviso Hosp, Microbiol Dept, Treviso, Italy
Rigoli, R:
Treviso Hosp, Microbiol Dept, Treviso, Italy
Carattoli, A:
Ist Super Sanita, Dept Infect Dis, Viale Regina Margherita 299, I-00161 Rome, Italy
Migliavacca, R:
Univ Pavia, Unit Microbiol & Clin Microbiol, Clin Surg Diagnost & Paediat Sci Dept, Pavia, Italy
Villa, L:
Ist Super Sanita, Dept Infect Dis, Viale Regina Margherita 299, I-00161 Rome, Italy
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