A Functional Virgin Olive Oil Enriched with Olive Oil and Thyme Phenolic Compounds Improves the Expression of Cholesterol Efflux-Related Genes: A Randomized, Crossover, Controlled Trial
Por:
Farras, M, Arranz, S, Carrion, S, Subirana, I, Munoz-Aguayo, D, Blanchart, G, Kool, M, Sola, R, Motilva, MJ, Escola-Gil, JC, Rubio, L, Fernandez-Castillejo, S, Pedret, A, Estruch, R, Covas, MI, Fito, M, Hernaez, A, Castaner, O
Publicada:
1 ago 2019
Resumen:
The consumption of antioxidant-rich foods such as virgin olive oil (VOO) promotes high-density lipoprotein (HDL) anti-atherogenic capacities. Intake of functional VOOs (enriched with olive/thyme phenolic compounds (PCs)) also improves HDL functions, but the gene expression changes behind these benefits are not fully understood. Our aim was to determine whether these functional VOOs could enhance the expression of cholesterol efflux-related genes. In a randomized, double-blind, crossover, controlled trial, 22 hypercholesterolemic subjects ingested for three weeks 25 mL/day of: (1) a functional VOO enriched with olive oil PCs (500 mg/kg); (2) a functional VOO enriched with olive oil (250 mg/kg) and thyme PCs (250 mg/kg; FVOOT), and; (3) a natural VOO (olive oil PCs: 80 mg/kg, control intervention). We assessed whether these interventions improved the expression of cholesterol efflux-related genes in peripheral blood mononuclear cells by quantitative reverse-transcription polymerase chain reactions. The FVOOT intervention upregulated the expression of CYP27A1 (p = 0.041 and p = 0.053, versus baseline and the control intervention, respectively), CAV1 (p = 0.070, versus the control intervention), and LXR beta, RXR alpha, and PPAR beta/delta (p = 0.005, p = 0.005, and p = 0.038, respectively, relative to the baseline). The consumption of a functional VOO enriched with olive oil and thyme PCs enhanced the expression of key cholesterol efflux regulators, such as CYP27A1 and nuclear receptor-related genes.
Filiaciones:
Farras, M:
IIB St Pau, Mol Bases Cardiovasc Risk Grp, Barcelona 08041, Spain
ISCIII, CIBER Diabet & Enfermedades Metab Asociadas CIB, Madrid 28029, Spain
Arranz, S:
Hosp Mar Med Res Inst IMIM, Cardiovasc Risk & Nutr Res Grp, Barcelona 08003, Spain
Carrion, S:
Hosp Mar Med Res Inst IMIM, Cardiovasc Risk & Nutr Res Grp, Barcelona 08003, Spain
Subirana, I:
ISCIII, CIBER Epidemiol & Salud PUbl CIBERESP, Madrid 28029, Spain
IMIM, Cardiovasc Genet & Epidemiol Res Grp, Barcelona 08003, Spain
Munoz-Aguayo, D:
Hosp Mar Med Res Inst IMIM, Cardiovasc Risk & Nutr Res Grp, Barcelona 08003, Spain
ISCIII, CIBER Fisiopatol Obesidad & Nutric CIBEROBN, Madrid 28029, Spain
Blanchart, G:
Hosp Mar Med Res Inst IMIM, Cardiovasc Risk & Nutr Res Grp, Barcelona 08003, Spain
Kool, M:
Hosp Mar Med Res Inst IMIM, Cardiovasc Risk & Nutr Res Grp, Barcelona 08003, Spain
Sola, R:
Univ Rovira & Virgili, Funct Nutr Oxidat & Cardiovasc Dis Grp, Reus 43201, Spain
Inst Invest Sanitaria Pere Virgili, Reus 43204, Spain
Hosp Univ St Joan Reus, Reus 43204, Spain
Motilva, MJ:
Univ la Rioja, CSIC, Gobierno La Rioja, ICVV, Logrono 26007, Spain
Escola-Gil, JC:
IIB St Pau, Mol Bases Cardiovasc Risk Grp, Barcelona 08041, Spain
ISCIII, CIBER Diabet & Enfermedades Metab Asociadas CIB, Madrid 28029, Spain
Rubio, L:
Univ Lleida, Agrotecnio Ctr, Food Technol Dept, Lleida 25198, Spain
Fernandez-Castillejo, S:
Univ Rovira & Virgili, Funct Nutr Oxidat & Cardiovasc Dis Grp, Reus 43201, Spain
Inst Invest Sanitaria Pere Virgili, Reus 43204, Spain
Pedret, A:
Univ Rovira & Virgili, Funct Nutr Oxidat & Cardiovasc Dis Grp, Reus 43201, Spain
Inst Invest Sanitaria Pere Virgili, Reus 43204, Spain
Estruch, R:
ISCIII, CIBER Fisiopatol Obesidad & Nutric CIBEROBN, Madrid 28029, Spain
Hosp Clin Barcelona, Internal Med Serv, E-08036 Barcelona, Spain
August Pi i Sunyer Biomed Res Inst IDIBAPS, Cardiovasc Risk Nutr & Aging Res Unit, Barcelona 08036, Spain
Covas, MI:
ISCIII, CIBER Fisiopatol Obesidad & Nutric CIBEROBN, Madrid 28029, Spain
NUPROAS Handelsbolag, Nacka, Sweden
NUPROAS HB, Apartado Correos 93, Girona 17242, Spain
Fito, M:
Hosp Mar Med Res Inst IMIM, Cardiovasc Risk & Nutr Res Grp, Barcelona 08003, Spain
ISCIII, CIBER Fisiopatol Obesidad & Nutric CIBEROBN, Madrid 28029, Spain
Hernaez, A:
ISCIII, CIBER Fisiopatol Obesidad & Nutric CIBEROBN, Madrid 28029, Spain
August Pi i Sunyer Biomed Res Inst IDIBAPS, Cardiovasc Risk Nutr & Aging Res Unit, Barcelona 08036, Spain
Castaner, O:
Hosp Mar Med Res Inst IMIM, Cardiovasc Risk & Nutr Res Grp, Barcelona 08003, Spain
ISCIII, CIBER Fisiopatol Obesidad & Nutric CIBEROBN, Madrid 28029, Spain
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