Altered microRNAs related to synaptic function as potential plasma biomarkers for Alzheimer's disease
Por:
Siedlecki-Wullich, D, Catala-Solsona, J, Fabregas, C, Hernandez, I, Clarimon, J, Lleo, A, Boada, M, Saura, CA, Rodriguez-Alvarez, J, Minano-Molina, AJ
Publicada:
15 may 2019
Resumen:
BackgroundSeveral evidences suggest that failure of synaptic function occurs at preclinical stages of Alzheimer's disease (AD) preceding neuronal loss and the classical AD pathological hallmarks. Nowadays, there is an urgent need to identify reliable biomarkers that could be obtained with non-invasive methods to improve AD diagnosis at early stages. Here, we have examined plasma levels of a group of miRNAs related to synaptic proteins in a cohort composed of cognitive healthy controls (HC), mild cognitive impairment (MCI) and AD subjects.MethodsPlasma and brain levels of miRNAs were analysed in two different cohorts including 38 HC, 26 MCI, 56AD dementia patients and 27 frontotemporal dementia (FTD) patients. D'Agostino and Pearson and Shapiro-Wilk tests were used to evaluate data normality. miRNA levels between groups were compared using a two-sided nonparametric Mann-Whitney test and sensitivity and specificity was determined by receiver operating characteristic curve analysis.ResultsSignificant upregulation of miR-92a-3p, miR-181c-5p and miR-210-3p was found in the plasma of both MCI and AD subjects. MCI patients that progress to AD showed higher plasma levels of these miRNAs. By contrast, no changes in miR-92a-3p, miR-181c-5p or miR-210-3p levels were observed in plasma obtained from a cohort of FTD.ConclusionOur study shows that plasma miR-92a-3p, miR-181c-5p and miR-210-3p constitute a specific molecular signature potentially useful as a potential biomarker for AD.
Filiaciones:
Siedlecki-Wullich, D:
Univ Autonoma Barcelona, Inst Neurociencies, Cerdanyola Del Valles 08193, Spain
Univ Autonoma Barcelona, Dept Bioquim & Biol Mol, Cerdanyola Del Valles 08193, Spain
Ctr Invest Biomed Red Enfermedades Neurodegenerat, Madrid, Spain
Catala-Solsona, J:
Univ Autonoma Barcelona, Inst Neurociencies, Cerdanyola Del Valles 08193, Spain
Univ Autonoma Barcelona, Dept Bioquim & Biol Mol, Cerdanyola Del Valles 08193, Spain
Ctr Invest Biomed Red Enfermedades Neurodegenerat, Madrid, Spain
Fabregas, C:
Univ Autonoma Barcelona, Inst Neurociencies, Cerdanyola Del Valles 08193, Spain
Univ Autonoma Barcelona, Dept Bioquim & Biol Mol, Cerdanyola Del Valles 08193, Spain
Ctr Invest Biomed Red Enfermedades Neurodegenerat, Madrid, Spain
Hernandez, I:
Univ Int Catalunya, Inst Catala Neurociencies Aplicades, Fundacio ACE, Res Ctr, Barcelona, Spain
Univ Int Catalunya, Inst Catala Neurociencies Aplicades, Fundacio ACE, Memory Clin, Barcelona, Spain
Clarimon, J:
Ctr Invest Biomed Red Enfermedades Neurodegenerat, Madrid, Spain
Univ Autonoma Barcelona, Hosp St Pau, Inst Invest Biomed St Pau, Dept Neurol,Memory Unit, Barcelona, Spain
Lleo, A:
Ctr Invest Biomed Red Enfermedades Neurodegenerat, Madrid, Spain
Univ Autonoma Barcelona, Hosp St Pau, Inst Invest Biomed St Pau, Dept Neurol,Memory Unit, Barcelona, Spain
Boada, M:
Ctr Invest Biomed Red Enfermedades Neurodegenerat, Madrid, Spain
Univ Int Catalunya, Inst Catala Neurociencies Aplicades, Fundacio ACE, Res Ctr, Barcelona, Spain
Univ Int Catalunya, Inst Catala Neurociencies Aplicades, Fundacio ACE, Memory Clin, Barcelona, Spain
Saura, CA:
Univ Autonoma Barcelona, Inst Neurociencies, Cerdanyola Del Valles 08193, Spain
Univ Autonoma Barcelona, Dept Bioquim & Biol Mol, Cerdanyola Del Valles 08193, Spain
Ctr Invest Biomed Red Enfermedades Neurodegenerat, Madrid, Spain
Rodriguez-Alvarez, J:
Univ Autonoma Barcelona, Inst Neurociencies, Cerdanyola Del Valles 08193, Spain
Univ Autonoma Barcelona, Dept Bioquim & Biol Mol, Cerdanyola Del Valles 08193, Spain
Ctr Invest Biomed Red Enfermedades Neurodegenerat, Madrid, Spain
Albert Einstein Coll Med, Dominick P Purpura Dept Neurosci, New York, NY 10461 USA
Minano-Molina, AJ:
Univ Autonoma Barcelona, Inst Neurociencies, Cerdanyola Del Valles 08193, Spain
Univ Autonoma Barcelona, Dept Bioquim & Biol Mol, Cerdanyola Del Valles 08193, Spain
Ctr Invest Biomed Red Enfermedades Neurodegenerat, Madrid, Spain
Gold, Green Published
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