Stem cells from human cardiac adipose tissue depots show different gene expression and functional capacities
Por:
Lambert, C, Arderiu, G, Bejar, MT, Crespo, J, Baldellou, M, Juan-Babot, O, Badimon, L
Publicada:
29 nov 2019
Resumen:
Background The composition and function of the adipose tissue covering the heart are poorly known. In this study, we have investigated the epicardial adipose tissue (EAT) covering the cardiac ventricular muscle and the EAT covering the left anterior descending artery (LAD) on the human heart, to identify their resident stem cell functional activity. Methods EAT covering the cardiac ventricular muscle was isolated from the apex (avoiding areas irrigated by major vessels) of the heart (ventricular myocardium adipose tissue (VMAT)) and from the area covering the epicardial arterial sulcus of the LAD (PVAT) in human hearts excised during heart transplant surgery. Adipose stem cells (ASCs) from both adipose tissue depots were immediately isolated and phenotypically characterized by flow cytometry. The different behavior of these ASCs and their released secretome microvesicles (MVs) were investigated by molecular and cellular analysis. Results ASCs from both VMAT (mASCs) and the PVAT (pASCs) were characterized by the expression of CD105, CD44, CD29, CD90, and CD73. The angiogenic-related genes VEGFA, COL18A1, and TF, as well as the miRNA126-3p and miRNA145-5p, were analyzed in both ASC types. Both ASCs were functionally able to form tube-like structures in three-dimensional basement membrane substrates. Interestingly, pASCs showed a higher level of expression of VEGFA and reduced level of COL18A1 than mASCs. Furthermore, MVs released by mASCs significantly induced human microvascular endothelial cell migration. Conclusion Our study indicates for the first time that the resident ASCs in human epicardial adipose tissue display a depot-specific angiogenic function. Additionally, we have demonstrated that resident stem cells are able to regulate microvascular endothelial cell function by the release of MVs.
Filiaciones:
Lambert, C:
IR Hosp Santa Creu & St Pau, IIB St Pau, Cardiovasc Program ICCC, C St Antoni Ma Claret 167, Barcelona 08025, Spain
Arderiu, G:
IR Hosp Santa Creu & St Pau, IIB St Pau, Cardiovasc Program ICCC, C St Antoni Ma Claret 167, Barcelona 08025, Spain
Bejar, MT:
IR Hosp Santa Creu & St Pau, IIB St Pau, Cardiovasc Program ICCC, C St Antoni Ma Claret 167, Barcelona 08025, Spain
Crespo, J:
IR Hosp Santa Creu & St Pau, IIB St Pau, Cardiovasc Program ICCC, C St Antoni Ma Claret 167, Barcelona 08025, Spain
Baldellou, M:
IR Hosp Santa Creu & St Pau, IIB St Pau, Cardiovasc Program ICCC, C St Antoni Ma Claret 167, Barcelona 08025, Spain
Juan-Babot, O:
IR Hosp Santa Creu & St Pau, IIB St Pau, Cardiovasc Program ICCC, C St Antoni Ma Claret 167, Barcelona 08025, Spain
Badimon, L:
IR Hosp Santa Creu & St Pau, IIB St Pau, Cardiovasc Program ICCC, C St Antoni Ma Claret 167, Barcelona 08025, Spain
Ciber CV, Madrid 28029, Spain
Cardiovasc Res Chair UAB, Barcelona, Spain
Gold, Green Published
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