A collection of three integration-free iPSCs derived from old male and female healthy subjects
Por:
Rodriguez-Traver, E, Diaz-Guerra, E, Rodriguez, C, Arenas, F, Orera, M, Kulisevsky, J, Moratalla, R, Vicario, C
Publicada:
1 ene 2020
Resumen:
Here, we present the characterization of three iPSC lines derived from dermal fibroblasts of old healthy subjects. Fibroblasts were reprogrammed using Sendai viral vectors encoding OCT4, SOX2, KLF4 and c-MYC. The iPSCs expressed endogenous pluripotency markers, could generate the three germ layers (ectoderm, mesoderm and endoderm), maintained a stable karyotype, and were free from Sendai vectors and reprogramming factors. These integration-free iPSCs can serve for establishing control cell cultures in studies searching for phenotypes and mechanisms that could potentially be dysregulated in degenerative diseases.
Filiaciones:
Rodriguez-Traver, E:
CSIC, Inst Cajal, Madrid, Spain
Ctr Invest Biomed Red Enfermedades Neurodegenerat, Madrid, Spain
Diaz-Guerra, E:
CSIC, Inst Cajal, Madrid, Spain
Ctr Invest Biomed Red Enfermedades Neurodegenerat, Madrid, Spain
Rodriguez, C:
Hosp Gen Univ Gregorio Maranon, Serv Bioquim Clin, Madrid, Spain
Arenas, F:
Ctr Invest Biomed Red Enfermedades Neurodegenerat, Madrid, Spain
Hosp Santa Creu & Sant Pau, Neurol Dept, Movement Disorders Unit, Barcelona, Spain
Orera, M:
Hosp Gen Univ Gregorio Maranon, Serv Bioquim Clin, Madrid, Spain
Kulisevsky, J:
Ctr Invest Biomed Red Enfermedades Neurodegenerat, Madrid, Spain
Hosp Santa Creu & Sant Pau, Neurol Dept, Movement Disorders Unit, Barcelona, Spain
Moratalla, R:
CSIC, Inst Cajal, Madrid, Spain
Ctr Invest Biomed Red Enfermedades Neurodegenerat, Madrid, Spain
Vicario, C:
CSIC, Inst Cajal, Madrid, Spain
Ctr Invest Biomed Red Enfermedades Neurodegenerat, Madrid, Spain
Gold
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