Physiological phenotyping of the adult zebrafish heart
Por:
Lin, E, Shafaattalab, S, Gill, J, Al-Zeer, B, Craig, C, Lamothe, M, Rayani, K, Gunawan, M, Li, AY, Hove-Madsen, L, Tibbits, GF
Publicada:
1 feb 2020
Resumen:
The zebrafish has proven to be an excellent organism for manipulation of its genome from a long history of transcript down-regulation using morpholino oligimers to more recent genome editing tools such as CRISPR-Cas9. Early forward and reverse genetic screens significantly benefited from the transparency of zebrafish embryos, allowing cardiac development as a function of genetics to be directly observed. However, gradual loss of transparency with subsequent maturation limited many of these approaches to the first several days post-fertilization. As many genes are developmentally regulated, the immature phenotype is not entirely indicative of that of the mature zebrafish. For accurate phenotyping, subsequent developmental stages including full maturation must also be considered.
In adult zebrafish, cardiac function can now be studied in great detail due both to the size of the hearts as well as recent technological improvements. Because of their small size, zebrafish are particularly amenable to high frequency echocardiography for detailed functional recordings. Although relatively small, the hearts are easily excised and contractile parameters can be measured from whole hearts, heart slices, individual cardiomyocytes and even single myofibrils. Similarly, electrical activity can also be measured using a variety of techniques, including in vivo and ex vivo electrocardiograms, optical mapping and traditional microelectrode techniques. In this report, the major advantages and technical considerations of these physiological tools are discussed.
Filiaciones:
Lin, E:
Simon Fraser Univ, Dept Biomed Physiol & Kinesiol, Mol Cardiac Physiol Grp, Burnaby, BC, Canada
British Columbia Childrens Hosp, Dept Cardiovasc Sci, Vancouver, BC, Canada
Shafaattalab, S:
Simon Fraser Univ, Dept Biomed Physiol & Kinesiol, Mol Cardiac Physiol Grp, Burnaby, BC, Canada
British Columbia Childrens Hosp, Dept Cardiovasc Sci, Vancouver, BC, Canada
Gill, J:
Simon Fraser Univ, Dept Biomed Physiol & Kinesiol, Mol Cardiac Physiol Grp, Burnaby, BC, Canada
Al-Zeer, B:
Simon Fraser Univ, Dept Biomed Physiol & Kinesiol, Mol Cardiac Physiol Grp, Burnaby, BC, Canada
Craig, C:
Simon Fraser Univ, Dept Biomed Physiol & Kinesiol, Mol Cardiac Physiol Grp, Burnaby, BC, Canada
Lamothe, M:
Simon Fraser Univ, Dept Biomed Physiol & Kinesiol, Mol Cardiac Physiol Grp, Burnaby, BC, Canada
Rayani, K:
Simon Fraser Univ, Dept Biomed Physiol & Kinesiol, Mol Cardiac Physiol Grp, Burnaby, BC, Canada
Gunawan, M:
Simon Fraser Univ, Dept Biomed Physiol & Kinesiol, Mol Cardiac Physiol Grp, Burnaby, BC, Canada
Li, AY:
Simon Fraser Univ, Dept Biomed Physiol & Kinesiol, Mol Cardiac Physiol Grp, Burnaby, BC, Canada
Hove-Madsen, L:
Hosp Santa Creu & Sant Pau, IIB St Pau, Biomed Res Inst Barcelona IIBB CSIC, Barcelona 08025, Spain
Hosp Santa Creu & Sant Pau, CIBERCV, Barcelona 08025, Spain
Tibbits, GF:
Simon Fraser Univ, Dept Biomed Physiol & Kinesiol, Mol Cardiac Physiol Grp, Burnaby, BC, Canada
British Columbia Childrens Hosp, Dept Cardiovasc Sci, Vancouver, BC, Canada
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