Candidate Transcript Panel in Semen Extracellular Vesicles Can Improve Prediction of Aggressiveness of Prostate Cancer
Por:
Ferre-Giraldo, A, Castells, M, Madurga, A, Arbiol-Roca, A, de Rocco-Ponce, M, Bassas, L, Vigués, F, Larriba, S
Publicada:
30 sep 2025
Resumen:
The need for prostate cancer (PCa)-specific biomarkers that enable more accurate detection of the disease and better prediction of tumor aggressiveness remains ongoing due to the low cancer specificity of PSA screening. Several potential mRNA markers for diagnosing PCa, in tissue and urine, have been reported in the literature. In this study, we aim to explore the potential of selected prostate-specific molecules and transcripts contained in small extracellular vesicles (sEVs) in semen to predict PCa risk reclassification for patients with moderately elevated PSA levels-a clinical scenario where identifying truly non-invasive biomarkers is especially critical. RT-qPCR analysis in semen sEVs successfully showed differential expression of KLK3 and PCA3 genes between PCa and healthy controls, whereas CREB3L4, CCNQ and DUSP23 levels were related to the severity or degree of PCa affectation. Our findings also present strong evidence that classifiers based on combined long transcript levels in semen sEVs serve as effective biomarkers. They can be used alone or in combination with blood PSA and/or semen citric acid levels to improve the diagnosis of PCa and assess its severity and disease progression with high accuracy. This strategy would allow a more comprehensive assessment, increase prognostic accuracy, and facilitate accurate clinical decision-making in the management of PCa.
Filiaciones:
Ferre-Giraldo, A:
Inst Invest Biomed Bellvitge IDIBELL, Immune Inflammatory Proc & Gene Therapeut Grp, Genes Dis & Therapy Program, Barcelona 08908, Spain
Castells, M:
Bellvitge Univ Hosp, Urol Serv, ICS, Barcelona 08908, Spain
Madurga, A:
Univ Hosp Bellvitge, Terr Clin Lab Metropolitan South, Barcelona 08908, Spain
Arbiol-Roca, A:
Univ Hosp Bellvitge, Terr Clin Lab Metropolitan South, Barcelona 08908, Spain
de Rocco-Ponce, M:
Univ Autonoma Barcelona, St Pau Hosp, Dept Neurol,St Pau IIB St Pau, Inst Invest Biosanitaries,Movement Disorders Unit, Barcelona 08025, Spain
Bassas, L:
Univ Autonoma Barcelona, St Pau Hosp, Dept Neurol,St Pau IIB St Pau, Inst Invest Biosanitaries,Movement Disorders Unit, Barcelona 08025, Spain
Vigués, F:
Bellvitge Univ Hosp, Urol Serv, ICS, Barcelona 08908, Spain
Larriba, S:
Inst Invest Biomed Bellvitge IDIBELL, Immune Inflammatory Proc & Gene Therapeut Grp, Genes Dis & Therapy Program, Barcelona 08908, Spain
Green Published, Green Submitted, gold
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