Platelet bound B cells and their role in SSc: Implications for disease subtypes and clinical outcomes
Por:
Osuna-Gómez, R, Castellví, I, Mulet, M, Tandaipan, JL, Zamora, C, Codes-Mendez, H, Ortiz, MA, Diaz-Torné, C, Cantó, E, Magallares, B, Guinart-Cuadra, A, Moya, P, Corominas, H, Vidal, S
Publicada:
1 ago 2025
Ahead of Print:
1 jul 2025
Resumen:
Objectives: Systemic sclerosis (SSc) is a complex autoimmune disease characterized by microvascular damage, immune dysregulation, and tissue fibrosis. While lymphocyte-platelet (PLT) complexes have been implicated in autoimmune diseases, their role in SSc is not well understood. Methods: In a study of 21 predominantly female SSc patients, 66.7 % had limited SSc (lcSSc), with anti-centromere antibodies (ACA) being the most common autoantibody pattern. We applied flow cytometry to analyze B cells with bound PLTs, enzyme-linked immunosorbent assay (ELISA) to determine plasma levels of activated PLT soluble factors, and co-culture assays to evaluate B cell cytokine secretion and plasma cell differentiation. Results: SSc patients had a higher percentage of B cells, but not T cells, with bound PLTs compared to healthy donors (HD). Despite similar PLT counts, SSc patients showed higher plasmatic levels of P-selectin (CD62P), soluble CD40 ligand (sCD40L), platelet-derived growth factor (PDGF), and transforming growth factor-beta (TGF-beta). Plasma IL-10 levels were also higher in SSc patients, with increased intracellular IL-10 in B cells with bound PLTs. We observed an increased IL-10 production and plasma cell differentiation when B cells were co-cultured with PLTs, especially from SSc patients. B cells with bound PLTs were associated with calcinosis, digital ulcers, and ACA status, with no effect from previous corticosteroid or aspirin therapy. Logistic regression identified B cells with bound PLTs as a predictor for distinguishing lcSSc patients. Conclusions: B cells with bound PLTs play a significant role in SSc by modulating B cell function and contributing to disease pathogenesis. Their association with clinical parameters suggests their potential as biomarkers for disease severity and subtype classification in SSc.
Filiaciones:
Osuna-Gómez, R:
Inst Recerca Hosp Santa Creu & St Pau, Biomed Res Inst St Pau IIB St Pau, Inflammatory Dis, Barcelona 08041, Spain
Castellví, I:
Hosp St Creu i St Pau, Dept Rheumatol & Syst Autoimmune Dis, Barcelona, Spain
Mulet, M:
Inst Recerca Hosp Santa Creu & St Pau, Biomed Res Inst St Pau IIB St Pau, Inflammatory Dis, Barcelona 08041, Spain
Tandaipan, JL:
Hosp St Creu i St Pau, Dept Rheumatol & Syst Autoimmune Dis, Barcelona, Spain
Zamora, C:
Inst Recerca Hosp Santa Creu & St Pau, Biomed Res Inst St Pau IIB St Pau, Inflammatory Dis, Barcelona 08041, Spain
Codes-Mendez, H:
Hosp St Creu i St Pau, Dept Rheumatol & Syst Autoimmune Dis, Barcelona, Spain
Ortiz, MA:
Inst Recerca Hosp Santa Creu & St Pau, Biomed Res Inst St Pau IIB St Pau, Inflammatory Dis, Barcelona 08041, Spain
Diaz-Torné, C:
Hosp St Creu i St Pau, Dept Rheumatol & Syst Autoimmune Dis, Barcelona, Spain
Cantó, E:
Inst Recerca Hosp Santa Creu & St Pau, Biomed Res Inst St Pau IIB St Pau, Inflammatory Dis, Barcelona 08041, Spain
Magallares, B:
Hosp St Creu i St Pau, Dept Rheumatol & Syst Autoimmune Dis, Barcelona, Spain
Guinart-Cuadra, A:
Inst Recerca Hosp Santa Creu & St Pau, Biomed Res Inst St Pau IIB St Pau, Inflammatory Dis, Barcelona 08041, Spain
Moya, P:
Hosp St Creu i St Pau, Dept Rheumatol & Syst Autoimmune Dis, Barcelona, Spain
Corominas, H:
Hosp St Creu i St Pau, Dept Rheumatol & Syst Autoimmune Dis, Barcelona, Spain
Vidal, S:
Inst Recerca Hosp Santa Creu & St Pau, Biomed Res Inst St Pau IIB St Pau, Inflammatory Dis, Barcelona 08041, Spain
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