Surpassing protein specificity in biomimetics of bacterial amyloids
Por:
Sanchez, JM, Voltà-Durán, E, Parladé, E, Mangues, R, Villaverde, A, Vázquez, E, Unzueta, U
Publicada:
1 mar 2025
Ahead of Print:
1 ene 2025
Resumen:
In nature, nontoxic protein amyloids serve as dynamic, protein-specific depots, exemplified by both bacterial inclusion bodies and secretory granules from the endocrine system. Inspired by these systems, chemically defined and regulatory-compliant artificial protein microgranules have been developed for clinical applications as endocrine-like protein repositories. This has been achieved by exploiting the reversible coordination between histidine residues and divalent cations such as Zn+2, that promotes protein-protein interactions. While stereo- specificity is a main architectonic feature of natural amyloids, the potential for synthetic approaches to create hybrid protein materials remains unexplored. Such materials could enable the occurrence and synchronized local application of diverse proteins in predefined molar ratios, for coupled enzymatic reactions or delivery of synergistically acting polypeptides. Here, we report on the fabrication of artificial protein granules with amyloidal architecture formed by combining two structurally distinct polypeptides. Specifically, we tested co-aggregation of the pairs GFP/IRFP and GFP/(3-galactosidase. The formation of hybrid microparticles was confirmed through FRET and complementary methodologies, demonstrating that the His-Zn clustering technology does not require sequential or structural homologies between aggregating polypeptides. This approach opens new avenues for the development of functional depots that capitalize on synergistic protein functionalities, paving the way for next- generation functional materials.
Filiaciones:
Sanchez, JM:
Univ Autonoma Barcelona, Inst Biotecnol & Biomed IBB, Barcelona, Spain
Inst Salud Carlos III, Ctr Invest Biomed Red Bioingn Biomat & Nanomed, Barcelona, Spain
Univ Nacl Cordoba, Fac Ciencias Exactas Fis & Nat, Dept Quim, Catedra Quim Biol,ICTA, Ave Velez Sarsfield 1611, RA-5016 Cordoba, Argentina
Univ Nacl Cordoba, Inst Invest Biol & Tecnol IIByT, CONICET, RA-5016 Cordoba, Argentina
Voltà-Durán, E:
Univ Autonoma Barcelona, Inst Biotecnol & Biomed IBB, Barcelona, Spain
Inst Salud Carlos III, Ctr Invest Biomed Red Bioingn Biomat & Nanomed, Barcelona, Spain
Univ Autonoma Barcelona, Dept Genet & Microbiol, Barcelona, Spain
Parladé, E:
Univ Autonoma Barcelona, Inst Biotecnol & Biomed IBB, Barcelona, Spain
Inst Salud Carlos III, Ctr Invest Biomed Red Bioingn Biomat & Nanomed, Barcelona, Spain
Univ Autonoma Barcelona, Dept Genet & Microbiol, Barcelona, Spain
Mangues, R:
Inst Salud Carlos III, Ctr Invest Biomed Red Bioingn Biomat & Nanomed, Barcelona, Spain
Inst Recerca St Pau IR St PAU, Barcelona 08041, Spain
Villaverde, A:
Univ Autonoma Barcelona, Inst Biotecnol & Biomed IBB, Barcelona, Spain
Inst Salud Carlos III, Ctr Invest Biomed Red Bioingn Biomat & Nanomed, Barcelona, Spain
Univ Autonoma Barcelona, Dept Genet & Microbiol, Barcelona, Spain
Vázquez, E:
Univ Autonoma Barcelona, Inst Biotecnol & Biomed IBB, Barcelona, Spain
Inst Salud Carlos III, Ctr Invest Biomed Red Bioingn Biomat & Nanomed, Barcelona, Spain
Univ Autonoma Barcelona, Dept Genet & Microbiol, Barcelona, Spain
Unzueta, U:
Inst Salud Carlos III, Ctr Invest Biomed Red Bioingn Biomat & Nanomed, Barcelona, Spain
Univ Autonoma Barcelona, Dept Genet & Microbiol, Barcelona, Spain
Inst Recerca St Pau IR St PAU, Barcelona 08041, Spain
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