Engineering Protein Nanoparticles Out from Components of the Human Microbiome
Por:
Lopez-Laguna, H, Sanchez-Garcia, L, Serna, N, Volta-Duran, E, Sanchez, JM, Sanchez-Chardi, A, Unzueta, U, Los, M, Villaverde, A, Vazquez, E
Publicada:
1 jul 2020
Ahead of Print:
1 jun 2020
Resumen:
Nanoscale protein materials are highly convenient as vehicles for targeted drug delivery because of their structural and functional versatility. Selective binding to specific cell surface receptors and penetration into target cells require the use of targeting peptides. Such homing stretches should be incorporated to larger proteins that do not interact with body components, to prevent undesired drug release into nontarget organs. Because of their low interactivity with human body components and their tolerated immunogenicity, proteins derived from the human microbiome are appealing and fully biocompatible building blocks for the biofabrication of nonreactive, inert protein materials within the nanoscale. Several phage and phage-like bacterial proteins with natural structural roles are produced inEscherichia colias polyhistidine-tagged recombinant proteins, looking for their organization as discrete, nanoscale particulate materials. While all of them self-assemble in a variety of sizes, the stability of the resulting constructs at 37 degrees C is found to be severely compromised. However, the fine adjustment of temperature and Zn(2+)concentration allows the formation of robust nanomaterials, fully stable in complex media and under physiological conditions. Then, microbiome-derived proteins show promise for the regulatable construction of scaffold protein nanomaterials, which can be tailored and strengthened by simple physicochemical approaches.
Filiaciones:
Lopez-Laguna, H:
Univ Autonoma Barcelona, Inst Biotecnol & Biomed, Barcelona 08193, Spain
Univ Autonoma Barcelona, Dept Gent & Microbiol, Barcelona 08193, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, C Monforte de Lemos 3-5, Madrid 28029, Spain
Sanchez-Garcia, L:
Univ Autonoma Barcelona, Inst Biotecnol & Biomed, Barcelona 08193, Spain
Univ Autonoma Barcelona, Dept Gent & Microbiol, Barcelona 08193, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, C Monforte de Lemos 3-5, Madrid 28029, Spain
Serna, N:
Univ Autonoma Barcelona, Inst Biotecnol & Biomed, Barcelona 08193, Spain
Univ Autonoma Barcelona, Dept Gent & Microbiol, Barcelona 08193, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, C Monforte de Lemos 3-5, Madrid 28029, Spain
Volta-Duran, E:
Univ Autonoma Barcelona, Inst Biotecnol & Biomed, Barcelona 08193, Spain
Univ Autonoma Barcelona, Dept Gent & Microbiol, Barcelona 08193, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, C Monforte de Lemos 3-5, Madrid 28029, Spain
Sanchez, JM:
Univ Autonoma Barcelona, Inst Biotecnol & Biomed, Barcelona 08193, Spain
Univ Autonoma Barcelona, Dept Gent & Microbiol, Barcelona 08193, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, C Monforte de Lemos 3-5, Madrid 28029, Spain
Univ Nacl Cordoba, Inst Invest Biol & Tecnol IIBYT, CONICET, ICTA, UNC Av Velez Sarsfield 1611,X 5016GCA, Cordoba, Argentina
Univ Nacl Cordoba, Inst Invest Biol & Tecnol IIBYT, CONICET, ICTA, Av Velez Sarsfield 1611,X 5016GCA, Cordoba, Argentina
UNC, Catedra Quim Biol, Dept Quim, FCEFyN, Av Velez Sarsfield 1611,X 5016GCA, Cordoba, Argentina
Sanchez-Chardi, A:
Univ Autonoma Barcelona, Serv Microscopia, Barcelona 08193, Spain
Univ Barcelona, Dept Biol Evolut Ecol & Ciencies Ambientals, Fac Biol, Av Diagonal 643, Barcelona 08028, Spain
Unzueta, U:
Univ Autonoma Barcelona, Dept Gent & Microbiol, Barcelona 08193, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, C Monforte de Lemos 3-5, Madrid 28029, Spain
Hosp Santa Creu & Sant Pau, Inst Invest Biomed St Pau, Barcelona 08041, Spain
Hosp Santa Creu & Sant Pau, Josep Carreras Res Inst, Barcelona 08041, Spain
Los, M:
Univ Gdansk, Dept Bacterial Mol Genet, Fac Biol, Wita Stwosza St 59, PL-80308 Gdansk, Poland
Phage Consultants, Partyzantow St 10-18, PL-80254 Gdansk, Poland
Villaverde, A:
Univ Autonoma Barcelona, Inst Biotecnol & Biomed, Barcelona 08193, Spain
Univ Autonoma Barcelona, Dept Gent & Microbiol, Barcelona 08193, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, C Monforte de Lemos 3-5, Madrid 28029, Spain
Vazquez, E:
Univ Autonoma Barcelona, Inst Biotecnol & Biomed, Barcelona 08193, Spain
Univ Autonoma Barcelona, Dept Gent & Microbiol, Barcelona 08193, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, C Monforte de Lemos 3-5, Madrid 28029, Spain
Green Accepted
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