Levels of Lysozyme and SLPI in Bronchoalveolar Lavage: Exploring Their Role in Interstitial Lung Disease
Por:
Osuna-Gómez, R, Mulet, M, Barril, S, Cantó, E, Millan-Billi, P, Pardessus, A, de la Rosa-Carrillo, D, Castillo, D, Vidal, S
Publicada:
1 abr 2024
Resumen:
Interstitial lung diseases (ILDs) are characterized by inflammation or fibrosis of the pulmonary parenchyma. Despite the involvement of immune cells and soluble mediators in pulmonary fibrosis, the influence of antimicrobial peptides (AMPs) remains underexplored. These effector molecules display a range of activities, which include immunomodulation and wound repair. Here, we investigate the role of AMPs in the development of fibrosis in ILD. We compare the concentration of different AMPs and different cytokines in 46 fibrotic (F-ILD) and 17 non-fibrotic (NF-ILD) patients by ELISA and using peripheral blood mononuclear cells from in vitro stimulation in the presence of lysozyme or secretory leukocyte protease inhibitor (SLPI) from 10 healthy donors. We observed that bronchoalveolar lavage (BAL) levels of AMPs were decreased in F-ILD patients (lysozyme: p < 0.001; SLPI: p < 0.001; LL-37: p < 0.001; lactoferrin: p = 0.47) and were negatively correlated with levels of TGF-beta (lysozyme: p = 0.02; SLPI: p < 0.001) and IL-17 (lysozyme: p < 0.001; SLPI: p < 0.001). We observed that lysozyme increased the percentage of CD86+ macrophages (p < 0.001) and the production of TNF-alpha (p < 0.001). We showed that lysozyme and SLPI were associated with clinical parameters (lysozyme: p < 0.001; SLPI: p < 0.001) and disease progression (lysozyme: p < 0.001; SLPI: p = 0.01). These results suggest that AMPs may play an important role in the anti-fibrotic response, regulating the effect of pro-fibrotic cytokines. In addition, levels of lysozyme in BAL may be a potential biomarker to predict the progression in F-ILD patients.
Filiaciones:
Osuna-Gómez, R:
Inst Recerca St Pau IR St Pau, Inflammatory Dis, Barcelona 08041, Spain
Mulet, M:
Inst Recerca St Pau IR St Pau, Inflammatory Dis, Barcelona 08041, Spain
Barril, S:
Univ Lleida UdL, Hosp Univ Arnau Vilanova Santa Maria, Inst Recerca Biomed Lleida IRBLleida, Resp Dept,Translat Res Resp Med, Lleida 25198, Spain
Hosp Santa Creu & Sant Pau, Dept Resp, Barcelona 08041, Spain
Cantó, E:
Inst Recerca St Pau IR St Pau, Inflammatory Dis, Barcelona 08041, Spain
Millan-Billi, P:
Hosp Santa Creu & Sant Pau, Dept Resp, Barcelona 08041, Spain
Hosp Badalona Germans Trias & Pujol, Dept Resp, Barcelona 08916, Spain
Pardessus, A:
Hosp Santa Creu & Sant Pau, Dept Resp, Barcelona 08041, Spain
de la Rosa-Carrillo, D:
Hosp Santa Creu & Sant Pau, Dept Resp, Barcelona 08041, Spain
Castillo, D:
Hosp Santa Creu & Sant Pau, Dept Resp, Barcelona 08041, Spain
Vidal, S:
Inst Recerca St Pau IR St Pau, Inflammatory Dis, Barcelona 08041, Spain
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