Properties and cellular uptake of photo-triggered mixed metallosurfactant vesicles intended for controlled CO delivery in gas therapy
Por:
Trallero J., Camacho M., Marín-García M., Álvarez-Marimon E., Benseny-Cases N., Barnadas-Rodríguez R.
Publicada:
1 ene 2023
Ahead of Print:
21 jun 2023
Resumen:
The scientific relevance of carbon monoxide has increased since it was discovered that it is a gasotransmitter involved in several biological processes. This fact stimulated research to find a secure and targeted delivery and lead to the synthesis of CO-releasing molecules. In this paper we present a vesicular CO delivery system triggered by light composed of a synthetized metallosurfactant (TCOL10) with two long carbon chains and a molybdenum-carbonyl complex. We studied the characteristics of mixed TCOL10/phosphatidylcholine metallosomes of different sizes. Vesicles from 80 to 800 nm in diameter are mainly unilamellar, do not disaggregate upon dilution, in the dark are physically and chemically stable at 4 °C for at least one month, and exhibit a lag phase of about 4 days before they show a spontaneous CO release at 37 °C. Internalization of metallosomes by cells was studied as function of the incubation time, and vesicle concentration and size. Results show that large vesicles are more efficiently internalized than the smaller ones in terms of the percentage of cells that show TCOL10 and the amount of drug that they take up. On balance, TCOL10 metallosomes constitute a promising and viable approach for efficient delivery of CO to biological systems.
Filiaciones:
Trallero J.:
Universitat Autònoma de Barcelona, Biophysics Unit/Center for Biophysical Studies, Department of Biochemistry and Molecular Biology, Faculty of Medicine, Cerdanyola del Vallès, 08193, Spain
Camacho M.:
Institut de Recerca de l'Hospital de la Santa Creu i Sant Pau - Centre CERCA, Genomics of Complex Diseases, Barcelona, Spain
Marín-García M.:
Universitat Autònoma de Barcelona, Biophysics Unit/Center for Biophysical Studies, Department of Biochemistry and Molecular Biology, Faculty of Medicine, Cerdanyola del Vallès, 08193, Spain
Álvarez-Marimon E.:
Universitat Autònoma de Barcelona, Biophysics Unit/Center for Biophysical Studies, Department of Biochemistry and Molecular Biology, Faculty of Medicine, Cerdanyola del Vallès, 08193, Spain
Benseny-Cases N.:
Universitat Autònoma de Barcelona, Biophysics Unit/Center for Biophysical Studies, Department of Biochemistry and Molecular Biology, Faculty of Medicine, Cerdanyola del Vallès, 08193, Spain
Consorcio para la Construcción Equipamiento y Explotacion del Laboratorio de Luz Sincrotron, ALBA Synchrotron Light Source, Catalonia, Cerdanyola del Vallès, 08290, Spain
Barnadas-Rodríguez R.:
Universitat Autònoma de Barcelona, Biophysics Unit/Center for Biophysical Studies, Department of Biochemistry and Molecular Biology, Faculty of Medicine, Cerdanyola del Vallès, 08193, Spain
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