Phenotypic and Molecular Characterization of an Enterobacter ludwigii Clinical Isolate Carrying a Plasmid-Mediated bla(IMI-6) Gene
Por:
Blanco-Martin, T, Guzman-Puche, J, Riazzo, C, Gasca-Santiyan, M, Hernandez-Garcia, M, Canton, R, Torre-Cisneros, J, Herrera, C, Martinez-Martinez, L
Publicada:
19 abr 2023
Ahead of Print:
1 abr 2023
Resumen:
IMI carbapenemases confer an unusual resistance pattern of susceptibility to broad-spectrum cephalosporins and piperacillin-tazobactam but decreased susceptibility to carbapenems, which may make them difficult to detect in routine practice. Commercially available molecular methods for the detection of carbapenemases in clinical laboratories do not usually include bla(IMI) genes, which could contribute to the hidden dissemination of bacteria producing these enzymes.
We report a plasmid-encoded IMI-6 carbapenemase in a clinical isolate of Enterobacter ludwigii from Spain. The isolate belongs to ST641 and was susceptible to expanded-spectrum cephalosporins and resistant to carbapenems. The modified carbapenem inactivation method (mCIM) test was positive, but beta-Carba was negative. Whole-genome sequencing identified the bla(IMI-6) gene located in a conjugative IncFIIY plasmid and associated with the LysR-like regulator imiR. Both genes were bracketed by an ISEclI-like insertion sequence and a putatively defective ISEc36 insertion sequence.IMPORTANCE IMI carbapenemases confer an unusual resistance pattern of susceptibility to broad-spectrum cephalosporins and piperacillin-tazobactam but decreased susceptibility to carbapenems, which may make them difficult to detect in routine practice. Commercially available molecular methods for the detection of carbapenemases in clinical laboratories do not usually include bla(IMI) genes, which could contribute to the hidden dissemination of bacteria producing these enzymes. Techniques should be implemented to detect minor carbapenemases that are not very frequent in our environment and control their dissemination.
Filiaciones:
Blanco-Martin, T:
Reina Sofia Univ Hosp, Microbiol Unit, Cordoba, Spain
Maimonides Biomed Res Inst Cordoba IMIBIC, Cordoba, Spain
Guzman-Puche, J:
Reina Sofia Univ Hosp, Microbiol Unit, Cordoba, Spain
Maimonides Biomed Res Inst Cordoba IMIBIC, Cordoba, Spain
Inst Salud Carlos III, CIBER Enfermedades Infecciosas CIBERINFEC, Madrid, Spain
Riazzo, C:
Reina Sofia Univ Hosp, Microbiol Unit, Cordoba, Spain
Maimonides Biomed Res Inst Cordoba IMIBIC, Cordoba, Spain
Gasca-Santiyan, M:
Reina Sofia Univ Hosp, Microbiol Unit, Cordoba, Spain
Maimonides Biomed Res Inst Cordoba IMIBIC, Cordoba, Spain
Hernandez-Garcia, M:
Inst Salud Carlos III, CIBER Enfermedades Infecciosas CIBERINFEC, Madrid, Spain
Ramon & Cajal Univ Hosp, Microbiol Unit, Madrid, Spain
Ramon & Cajal Inst Hlth Reasearch IRICYS, Madrid, Spain
Canton, R:
Inst Salud Carlos III, CIBER Enfermedades Infecciosas CIBERINFEC, Madrid, Spain
Ramon & Cajal Univ Hosp, Microbiol Unit, Madrid, Spain
Ramon & Cajal Inst Hlth Reasearch IRICYS, Madrid, Spain
Torre-Cisneros, J:
Maimonides Biomed Res Inst Cordoba IMIBIC, Cordoba, Spain
Inst Salud Carlos III, CIBER Enfermedades Infecciosas CIBERINFEC, Madrid, Spain
Reina Sofia Univ Hosp, Infect Dis Unit, Cordoba, Spain
Univ Cordoba, Med & Surg Sci Dept, Cordoba, Spain
Herrera, C:
Maimonides Biomed Res Inst Cordoba IMIBIC, Cordoba, Spain
Reina Sofia Univ Hosp, Haematol Dept, Cordoba, Spain
Martinez-Martinez, L:
Reina Sofia Univ Hosp, Microbiol Unit, Cordoba, Spain
Maimonides Biomed Res Inst Cordoba IMIBIC, Cordoba, Spain
Inst Salud Carlos III, CIBER Enfermedades Infecciosas CIBERINFEC, Madrid, Spain
Univ Cordoba, Soil Sci & Microbiol Dept, Agr Chem, Cordoba, Spain
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