Allergen sensitization stratifies IL-31 production by memory T cells in atopic dermatitis patients
Por:
Sans-de San Nicolàs L., Figueras-Nart I., García-Jiménez I., Bonfill-Ortí M., Guilabert A., Curto-Barredo L., Bertolín-Colilla M., Ferran M., Serra-Baldrich E., Pujol R.M., Santamaria-Babí L.F.
Publicada:
13 mar 2023
Ahead of Print:
13 mar 2023
Resumen:
BackgroundThe role of allergen sensitization in IL-31 production by T cells and specifically in the clinical context of atopic dermatitis (AD) has not been characterized. MethodsThe response to house dust mite (HDM) in purified memory T cells cocultured with epidermal cells from AD patients (n=58) and control subjects (n=11) was evaluated. AD-associated cytokines from culture supernatants, plasma proteins and mRNA expression from cutaneous lesions were assessed and related with the clinical features of the patients. ResultsHDM-induced IL-31 production by memory T cells defined two subsets of AD patients according to the presence or absence of IL-31 response. Patients in the IL-31 producing group showed a more inflammatory profile, and increased HDM-specific (sp) and total IgE levels compared to the IL-31 non-producing group. A correlation between IL-31 production and patient's pruritus intensity, plasma CCL27 and periostin was detected. When the same patients were analyzed based on sp IgE and total IgE levels, an increased IL-31 in vitro response, as well as type 2 markers in plasma and cutaneous lesions, was found in patients with sp IgE levels > 100 kUA/L and total IgE levels > 1000 kU/L. The IL-31 response by memory T cells was restricted to the cutaneous lymphocyte-associated antigen (CLA)(+) T-cell subset. ConclusionIgE sensitization to HDM allows stratifying IL-31 production by memory T cells in AD patients and relating it to particular clinical phenotypes of the disease.
Filiaciones:
Sans-de San Nicolàs L.:
Univ Barcelona UB, Dept Biol Cellular Fisiol & Immunol, Immunol Translac, Parc Cient Barcelona PCB, Barcelona, Spain
Figueras-Nart I.:
Univ Barcelona UB, Hosp Bellvitge, Dept Dermatol, Lhospitalet De Llobregat, Spain
García-Jiménez I.:
Univ Barcelona UB, Dept Biol Cellular Fisiol & Immunol, Immunol Translac, Parc Cient Barcelona PCB, Barcelona, Spain
Bonfill-Ortí M.:
Univ Barcelona UB, Hosp Bellvitge, Dept Dermatol, Lhospitalet De Llobregat, Spain
Guilabert A.:
Hosp Gen Granollers, Dept Dermatol, Granollers, Spain
Curto-Barredo L.:
Univ Autonoma Barcelona UAB, Hosp Mar, Inst Hosp Mar Invest Med IMIM, Dept Dermatol, Barcelona, Spain
Bertolín-Colilla M.:
Univ Autonoma Barcelona UAB, Hosp Mar, Inst Hosp Mar Invest Med IMIM, Dept Dermatol, Barcelona, Spain
Ferran M.:
Univ Autonoma Barcelona UAB, Hosp Mar, Inst Hosp Mar Invest Med IMIM, Dept Dermatol, Barcelona, Spain
Serra-Baldrich E.:
Univ Autonoma Barcelona UAB, Hosp Santa Creu & St Pau, Dept Dermatol, Barcelona, Spain
Pujol R.M.:
Univ Autonoma Barcelona UAB, Hosp Mar, Inst Hosp Mar Invest Med IMIM, Dept Dermatol, Barcelona, Spain
Santamaria-Babí L.F.:
Univ Barcelona UB, Dept Biol Cellular Fisiol & Immunol, Immunol Translac, Parc Cient Barcelona PCB, Barcelona, Spain
Green Published, gold, All Open Access, Gold, Green
|