The Effects of Interstitial Lung Diseases on Alveolar Extracellular Vesicles Profile: A Multicenter Study
Por:
d'Alessandro, M, Gangi, S, Soccio, P, Canto, E, Osuna-Gomez, R, Bergantini, L, Cameli, P, Fabbri, G, Croce, S, Scioscia, G, Montuori, G, Fanetti, M, Moriondo, G, Mezzasalma, F, Castillo, D, Lacedonia, D, Vidal, S, Bargagli, E
Publicada:
1 feb 2023
Ahead of Print:
17 feb 2023
Resumen:
Diagnosis of interstitial lung diseases (ILD) is difficult to perform. Extracellular vesicles (EVs) facilitate cell-to-cell communication, and they are released by a variety of cells. Our goal aimed to investigate EV markers in bronchoalveolar lavage (BAL) from idiopathic pulmonary fibrosis (IPF), sarcoidosis and hypersensitivity pneumonitis (HP) cohorts. ILD patients followed at Siena, Barcelona and Foggia University Hospitals were enrolled. BAL supernatants were used to isolate the EVs. They were characterized by flow cytometry assay through MACSPlex Exsome KIT. The majority of alveolar EV markers were related to the fibrotic damage. CD56, CD105, CD142, CD31 and CD49e were exclusively expressed by alveolar samples from IPF patients, while HP showed only CD86 and CD24. Some EV markers were common between HP and sarcoidosis (CD11c, CD1c, CD209, CD4, CD40, CD44, CD8). Principal component analysis distinguished the three groups based on EV markers with total variance of 60.08%. This study has demonstrated the validity of the flow cytometric method to phenotype and characterize EV surface markers in BAL samples. The two granulomatous diseases, sarcoidosis and HP, cohorts shared alveolar EV markers not revealed in IPF patients. Our findings demonstrated the viability of the alveolar compartment allowing identification of lung-specific markers for IPF and HP.
Filiaciones:
d'Alessandro, M:
Univ Siena, Dept Med & Surg Sci & Neurosci, Resp Dis & Lung Transplantat Unit, I-53100 Siena, Italy
Gangi, S:
Univ Siena, Dept Med & Surg Sci & Neurosci, Resp Dis & Lung Transplantat Unit, I-53100 Siena, Italy
Soccio, P:
Univ Foggia, Dept Med & Surg Sci, I-71122 Foggia, Italy
Policlin Riuniti Foggia, Inst Resp Dis, I-71122 Foggia, Italy
Canto, E:
Biomed Res Inst St Pau IIB St Pau, Inflammatory Dis, Barcelona 08041, Spain
Osuna-Gomez, R:
Biomed Res Inst St Pau IIB St Pau, Inflammatory Dis, Barcelona 08041, Spain
Bergantini, L:
Univ Siena, Dept Med & Surg Sci & Neurosci, Resp Dis & Lung Transplantat Unit, I-53100 Siena, Italy
Cameli, P:
Univ Siena, Dept Med & Surg Sci & Neurosci, Resp Dis & Lung Transplantat Unit, I-53100 Siena, Italy
Fabbri, G:
Univ Siena, Dept Med & Surg Sci & Neurosci, Resp Dis & Lung Transplantat Unit, I-53100 Siena, Italy
Croce, S:
Univ Siena, Dept Med & Surg Sci & Neurosci, Resp Dis & Lung Transplantat Unit, I-53100 Siena, Italy
Scioscia, G:
Univ Foggia, Dept Med & Surg Sci, I-71122 Foggia, Italy
Policlin Riuniti Foggia, Inst Resp Dis, I-71122 Foggia, Italy
Montuori, G:
Univ Siena, Dept Med & Surg Sci & Neurosci, Resp Dis & Lung Transplantat Unit, I-53100 Siena, Italy
Fanetti, M:
Univ Siena, Dept Med & Surg Sci & Neurosci, Resp Dis & Lung Transplantat Unit, I-53100 Siena, Italy
Moriondo, G:
Univ Foggia, Dept Med & Surg Sci, I-71122 Foggia, Italy
Policlin Riuniti Foggia, Inst Resp Dis, I-71122 Foggia, Italy
Mezzasalma, F:
Univ Hosp Siena Azienda Osped Univ Senese AOUS, Cardiothorac & Vasc Dept, Diagnost & Intervent Bronchoscopy Unit, I-53100 Siena, Italy
Castillo, D:
Hosp Santa Creu & Sant Pau, St Pau Biomed Res Inst IIB St Pau, Resp Dept, Barcelona 08041, Spain
Lacedonia, D:
Univ Foggia, Dept Med & Surg Sci, I-71122 Foggia, Italy
Policlin Riuniti Foggia, Inst Resp Dis, I-71122 Foggia, Italy
Vidal, S:
Biomed Res Inst St Pau IIB St Pau, Inflammatory Dis, Barcelona 08041, Spain
Bargagli, E:
Univ Siena, Dept Med & Surg Sci & Neurosci, Resp Dis & Lung Transplantat Unit, I-53100 Siena, Italy
Green Published, gold, All Open Access, Gold, Green
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