Usefulness of Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry in the Characterization of Leishmania Strains Causing Tegumentary Leishmaniasis in Bolivia versus hsp70 Gene Sequencing
Por:
Torrico M.C., Fernández-Arévalo A., Ballart C., Solano M., Rojas E., Abras A., Gonzales F., Arnau A., Tebar S., Llovet T., Lozano D., Ariza-Vioque E., Gascón J., Picado A., Torrico F., Munoz, C, Gállego M.
Publicada:
1 ene 2023
Ahead of Print:
1 ene 2023
Resumen:
The objective of the study was to evaluate the usefulness of MALDI-TOF MS for the characterization of Leishmania species circulating in Bolivia, in comparison with the sequencing of the hsp70 gene. In our study, all of the isolates could be identified, and no misidentifications were observed at the complex level.
Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) is a proteomic technique with proven efficiency in the identification of microorganisms, such as bacteria, fungi, and parasites. The present study aimed to evaluate the usefulness of MALDI-TOF MS for the characterization of Leishmania species circulating in Bolivia using hsp70 gene sequencing as a reference technique. 55 Leishmania strains that were isolated from patients with tegumentary leishmaniasis were analyzed. MALDI-TOF MS identified two species of the L. braziliensis complex (L. braziliensis, n = 26; L. braziliensis outlier, n = 18), one species of the L. guyanensis complex (L. guyanensis, n = 1), one species of the L. lainsoni complex (L. lainsoni, n = 2), and two species of the L. mexicana complex (L. amazonensis, n = 5; and L. garnhami, n = 3). All of the strains were correctly identified at the subgenus, genus, and complex level, but 10 of them (18%) were misidentified as other species within the same complex by the hsp70 gene sequencing, with 7 of these corresponding to possible hybrids. Thus, one L. braziliensis corresponded to L. peruviana, two L. braziliensis corresponded to L. braziliensis/L. peruviana possible hybrids, two L. amazonensis corresponded to L. mexicana, and three L. garnhami and two L. amazonensis corresponded to L. mexicana/L. amazonensis possible hybrids. Accordingly, MALDI-TOF MS could be used as an alternative to molecular techniques for the identification of Leishmania spp., as it is low cost, simple to apply, and able to quickly produce results. In Bolivia, its application would allow for the improvement of the management of patient follow-ups, the updating of the epidemiological data of the Leishmania species, and a contribution to the control of tegumentary leishmaniasis.IMPORTANCE The objective of the study was to evaluate the usefulness of MALDI-TOF MS for the characterization of Leishmania species circulating in Bolivia, in comparison with the sequencing of the hsp70 gene. In our study, all of the isolates could be identified, and no misidentifications were observed at the complex level. Although the equipment implies a high initial investment in our context, MALDI-TOF MS can be used in different areas of microbiology and significantly reduces the cost of testing. Once the parasite culture is obtained, the technique quickly yields information by accessing a free database that is available online. This would allow for the improvement of the management of patients and follow-ups, the updating of the epidemiological data of the species, and a contribution to the control of tegumentary leishmaniasis in Bolivia. Likewise, it can be used to determine a specific treatment to be given, according to the causal species of Leishmania, when there are protocols in this regard in the area.
Filiaciones:
Torrico M.C.:
Univ Mayor San Simon, Fac Med, Cochabamba, Bolivia
Fdn CEADES & Medio Ambiente, Cochabamba, Bolivia
Univ Barcelona, Dept Biol, Seccio Parasitol, Sanitat & Med Ambient,Fac Farm, Barcelona, Spain
Fernández-Arévalo A.:
Univ Barcelona, Dept Biol, Seccio Parasitol, Sanitat & Med Ambient,Fac Farm, Barcelona, Spain
Ballart C.:
Univ Barcelona, Dept Biol, Seccio Parasitol, Sanitat & Med Ambient,Fac Farm, Barcelona, Spain
Inst Salud Global Barcelona ISGlobal, Barcelona, Spain
Solano M.:
Univ Mayor San Simon, Fac Med, Cochabamba, Bolivia
Rojas E.:
Univ Mayor San Simon, Fac Med, Cochabamba, Bolivia
Abras A.:
Univ Girona, Dept Biol, Girona, Spain
Gonzales F.:
Univ Mayor San Simon, Fac Med, Cochabamba, Bolivia
Arnau A.:
Univ Barcelona, Dept Biol, Seccio Parasitol, Sanitat & Med Ambient,Fac Farm, Barcelona, Spain
Univ Girona, Dept Biol, Girona, Spain
Tebar S.:
Univ Barcelona, Dept Biol, Seccio Parasitol, Sanitat & Med Ambient,Fac Farm, Barcelona, Spain
Llovet T.:
Hosp Santa Creu & Sant Pau Barcelona, Serv Microbiol, Barcelona, Spain
Univ Autonoma Barcelona, Dept Genet & Microbiol, Bellaterra, Spain
Lozano D.:
Univ Mayor San Simon, Fac Med, Cochabamba, Bolivia
Fdn CEADES & Medio Ambiente, Cochabamba, Bolivia
Ariza-Vioque E.:
Univ Barcelona, Dept Biol, Seccio Parasitol, Sanitat & Med Ambient,Fac Farm, Barcelona, Spain
Inst Invest Biomed August Pi I Sunyer IDIBAPS, Barcelona, Spain
Gascón J.:
Inst Salud Global Barcelona ISGlobal, Barcelona, Spain
Inst Salud Carlos III, CIBERINFEC, ISCIII CIBER Enfermedades Infecciosas, Madrid, Spain
Picado A.:
Inst Salud Global Barcelona ISGlobal, Barcelona, Spain
Torrico F.:
Univ Mayor San Simon, Fac Med, Cochabamba, Bolivia
Fdn CEADES & Medio Ambiente, Cochabamba, Bolivia
Munoz, C:
Hosp Santa Creu & Sant Pau Barcelona, Serv Microbiol, Barcelona, Spain
Univ Autonoma Barcelona, Dept Genet & Microbiol, Bellaterra, Spain
Inst Recerca Biomed St Pau, Barcelona, Spain
Gállego M.:
Univ Barcelona, Dept Biol, Seccio Parasitol, Sanitat & Med Ambient,Fac Farm, Barcelona, Spain
Inst Salud Global Barcelona ISGlobal, Barcelona, Spain
Inst Salud Carlos III, CIBERINFEC, ISCIII CIBER Enfermedades Infecciosas, Madrid, Spain
gold, Green Accepted, All Open Access, Gold, Green
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