Cord Blood Platelet Rich Plasma Derivatives for Clinical Applications in Non-transfusion Medicine
Por:
Samarkanova, D, Cox, S, Hernandez, D, Rodriguez, L, Casaroli-Marano, RP, Madrigal, A, Querol, S
Publicada:
27 may 2020
Resumen:
Cord blood platelet rich plasma (CB-PRP) derivatives have been investigated as potential therapeutic agents for the treatment of diverse conditions including ocular surface disease and skin ulcers. We have developed processes for the formulation of several CB-PRP preparations, which have different composition and attributes. Here we describe the molecular characteristics of these preparations and we make recommendations as to their most appropriate clinical application based on functional and immunomodulatory profiles. We show that incubation of adult peripheral blood mononuclear cells (PBMCs) with all three preparations dramatically reduced the production of INF gamma and the expression of NKG2D and CD107a in NK, NKT, and T cells thus diminishing their activation, we propose that the likely mechanism is the high levels of soluble NKG2D ligands present in plasma. Of the three preparations we investigated, CB platelet lysate (PL) and platelet releaseate (PR) have higher concentrations of trophic and pro-angiogenic factors, CB platelet poor plasma (PPP) has the lowest concentration of all analytes measured. Based on these finding we propose that CB-PR is the most suitable raw material for skin wound patches, while CB-PL and PPP can be used to prepare eye drops for severe ocular surface pathologies and inflammatory conditions such as corneal ulcers or severe dry eye disease, respectively.
Filiaciones:
Samarkanova, D:
Banc Sang & Teixits, Barcelona, Spain
Vall dHebron Res Inst VHIR, Transfus Med Res Grp, Barcelona, Spain
Univ Autonoma Barcelona UAB, Dept Med, Barcelona, Spain
Cox, S:
Royal Free Hosp, Anthony Nolan Res Inst, London, England
Royal Free Hosp, UCL Canc Inst, London, England
Hernandez, D:
Royal Free Hosp, Anthony Nolan Res Inst, London, England
Royal Free Hosp, UCL Canc Inst, London, England
Rodriguez, L:
Banc Sang & Teixits, Barcelona, Spain
Casaroli-Marano, RP:
Banc Sang & Teixits, Barcelona, Spain
Univ Barcelona, Sch Med, Dept Surg, Barcelona, Spain
Univ Barcelona, Hosp Clin Barcelona, Barcelona, Spain
Madrigal, A:
Banc Sang & Teixits, Barcelona, Spain
Royal Free Hosp, Anthony Nolan Res Inst, London, England
Royal Free Hosp, UCL Canc Inst, London, England
Querol, S:
Banc Sang & Teixits, Barcelona, Spain
Vall dHebron Res Inst VHIR, Transfus Med Res Grp, Barcelona, Spain
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